##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1781216_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	172109
Sequences flagged as poor quality	0
Sequence length	76
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.27997373757328	34.0	34.0	34.0	32.0	34.0
2	33.307462131556164	34.0	34.0	34.0	33.0	34.0
3	33.34544968595483	34.0	34.0	34.0	33.0	34.0
4	33.394505807366265	34.0	34.0	34.0	33.0	34.0
5	33.40520251700957	34.0	34.0	34.0	34.0	34.0
6	36.830909481781894	38.0	37.0	38.0	34.0	38.0
7	36.72536590184127	38.0	37.0	38.0	34.0	38.0
8	36.49056702438571	38.0	37.0	38.0	34.0	38.0
9	36.470353090192845	38.0	37.0	38.0	34.0	38.0
10-11	36.37530576553231	38.0	37.0	38.0	34.0	38.0
12-13	36.31490799435242	38.0	37.0	38.0	34.0	38.0
14-15	36.33038365222039	38.0	37.0	38.0	34.0	38.0
16-17	36.25911195811956	38.0	37.0	38.0	34.0	38.0
18-19	36.25896670133462	38.0	37.0	38.0	34.0	38.0
20-21	36.182994497672986	38.0	37.0	38.0	33.5	38.0
22-23	36.26187183703351	38.0	37.0	38.0	34.0	38.0
24-25	36.24836876630508	38.0	37.0	38.0	34.0	38.0
26-27	36.01651279131248	38.0	36.5	38.0	32.0	38.0
28-29	36.01003724383966	38.0	37.0	38.0	32.0	38.0
30-31	36.0018447611688	38.0	37.0	38.0	32.0	38.0
32-33	35.95544393378614	38.0	37.0	38.0	32.0	38.0
34-35	35.97450162397085	38.0	37.0	38.0	32.0	38.0
36-37	35.922752441766555	38.0	36.0	38.0	32.0	38.0
38-39	35.72784688772813	38.0	36.0	38.0	31.0	38.0
40-41	35.857314260149096	38.0	36.0	38.0	31.5	38.0
42-43	35.90403174732292	38.0	36.0	38.0	32.0	38.0
44-45	35.87450685321511	38.0	36.0	38.0	31.0	38.0
46-47	35.901977816383805	38.0	36.5	38.0	31.5	38.0
48-49	35.8711427060758	38.0	36.0	38.0	31.5	38.0
50-51	35.765506161792814	38.0	36.0	38.0	31.0	38.0
52-53	35.773852616655724	38.0	36.0	38.0	31.0	38.0
54-55	35.82392844069747	38.0	36.0	38.0	31.0	38.0
56-57	35.76863789807622	38.0	36.0	38.0	31.0	38.0
58-59	35.73008674735197	38.0	36.0	38.0	31.0	38.0
60-61	35.72677779779094	38.0	36.0	38.0	31.0	38.0
62-63	35.68424370602351	38.0	36.0	38.0	31.0	38.0
64-65	35.64380131195928	38.0	36.0	38.0	31.0	38.0
66-67	35.59561092098612	38.0	36.0	38.0	31.0	38.0
68-69	35.44817818940322	38.0	36.0	38.0	31.0	38.0
70-71	35.24289839578407	38.0	36.0	38.0	28.5	38.0
72-73	35.059703443747864	38.0	36.0	38.0	27.0	38.0
74-75	35.07169293877717	38.0	36.0	38.0	27.0	38.0
76	34.082215340278545	37.0	34.0	38.0	24.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	0.0
19	0.0
20	2.0
21	10.0
22	32.0
23	55.0
24	149.0
25	280.0
26	546.0
27	957.0
28	1590.0
29	2417.0
30	3761.0
31	5536.0
32	7887.0
33	10920.0
34	14334.0
35	20253.0
36	28906.0
37	74473.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.900719892626185	18.282599980245077	9.955900040090873	28.860780087037863
2	22.349208931549192	19.079769216020082	32.95004909679331	25.620972755637418
3	20.010574693943955	21.116269340940917	29.49003247941711	29.383123485698015
4	14.561121149968914	18.058904531430663	34.431087276086664	32.94888704251376
5	14.407148957927825	33.219064662510384	35.985334874992006	16.38845150456978
6	31.570109639821275	37.88122643208665	16.167080164314477	14.381583763777606
7	29.694554032618864	27.527322801248044	20.447507103056783	22.330616063076306
8	26.10264425451313	35.45020887925675	17.770134042961146	20.677012823268974
9	23.89299804193854	15.242665984928156	19.614314184615562	41.25002178851774
10-11	25.74095486000151	24.94581921921573	26.45009848410019	22.863127436682568
12-13	25.311285290135903	21.62437757467651	26.236861523801775	26.827475611385807
14-15	23.314295010719952	22.691724430448147	21.932031445188805	32.061949113643095
16-17	25.607609131422528	26.83473845063303	23.134757624528643	24.4228947934158
18-19	25.192755753621256	24.9960780668065	24.135286359225837	25.67587982034641
20-21	23.176591578592635	25.01350888099983	24.336612263158813	27.47328727724872
22-23	23.82472735301466	26.03117791632047	24.446716906146687	25.69737782451818
24-25	25.238947411233582	26.189798325479785	24.151555119139616	24.41969914414702
26-27	24.709631686896095	26.626149707452836	24.373507486534695	24.290711119116374
28-29	25.120998901858705	24.70614552405743	25.056504889343383	25.116350684740485
30-31	23.942385348819645	26.115136338018345	24.555950008424894	25.386528304737116
32-33	22.972941566100552	25.060862592891713	27.69117245466536	24.275023386342376
34-35	25.530913548971874	25.82491328169939	25.26887030893213	23.37530286039661
36-37	24.24335740722449	25.51638787047743	26.04308897268591	24.197165749612164
38-39	23.08420826336798	25.098629356977263	26.644161548785945	25.17300083086881
40-41	23.249800998204627	23.763428989768112	27.253077991272974	25.73369202075429
42-43	24.750303586680534	23.736701741338337	26.838515127041585	24.674479544939544
44-45	22.21382960798099	25.572747503035863	27.55172594111871	24.661696947864435
46-47	24.3238296660837	23.33724558274117	26.237733064511442	26.101191686663682
48-49	23.33956969130028	23.448802793578487	26.684252421430603	26.527375093690626
50-51	21.667083069450175	23.67017413383379	28.169648304272293	26.493094492443742
52-53	21.21097676472468	23.565589248673806	30.22968002835412	24.99375395824739
54-55	20.616876514301982	23.89997036761587	28.700126082889334	26.783027035192813
56-57	21.901527520350474	24.020242985549856	27.094748095683553	26.98348139841612
58-59	20.672074092580864	23.82036964946633	29.397358650622568	26.11019760733024
60-61	22.035744789639125	25.174162885148366	26.48089292250841	26.3091994027041
62-63	19.79675670650576	26.96634109779268	26.235699469522224	27.00120272617934
64-65	20.24966736196248	27.35185260503518	25.212510676373697	27.185969356628647
66-67	20.40305852686379	27.269056237616855	24.805501164959416	27.522384070559937
68-69	20.19853697366204	27.70744121457913	24.427252496964133	27.666769314794692
70-71	20.886763620728725	26.950653365018677	24.12221324858084	28.040369765671752
72-73	21.120627044489247	25.394662684694	24.33051147819115	29.154198792625603
74-75	21.15781278143502	25.563451068799424	24.37263594582503	28.906100203940525
76	21.530541691602416	25.439692288026773	24.272408764213377	28.757357256157434
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.5
12	1.0
13	1.0
14	3.5
15	10.5
16	19.5
17	24.0
18	33.5
19	54.0
20	78.0
21	91.0
22	112.5
23	194.0
24	335.5
25	417.0
26	516.0
27	679.0
28	874.5
29	1006.0
30	1167.5
31	1488.0
32	2063.0
33	2479.0
34	2742.5
35	3398.0
36	4315.5
37	4841.0
38	5292.0
39	6690.0
40	8655.5
41	10125.0
42	10576.0
43	10850.5
44	10827.0
45	10278.5
46	10028.0
47	9885.0
48	9092.5
49	8098.0
50	7753.0
51	7206.5
52	6097.5
53	5068.5
54	4602.0
55	4258.5
56	3558.0
57	2997.0
58	2793.0
59	2583.0
60	2287.5
61	2150.0
62	2098.0
63	2081.0
64	2109.5
65	2215.0
66	2227.5
67	2180.0
68	2208.5
69	2165.5
70	2145.5
71	2197.0
72	2132.5
73	2023.0
74	1838.5
75	1699.0
76	1570.5
77	1352.5
78	1123.5
79	984.0
80	882.0
81	686.0
82	490.0
83	388.0
84	306.5
85	197.5
86	126.0
87	82.0
88	66.5
89	43.0
90	20.5
91	5.0
92	4.0
93	2.5
94	1.0
95	1.5
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	172109.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.72107792154972
#Duplication Level	Percentage of deduplicated	Percentage of total
1	63.5762449817459	28.43198205788192
2	18.918005950447583	16.92067236460615
3	6.930062752536735	9.29759629072274
4	2.8803804128935027	5.152548675548635
5	1.6318257999974015	3.64885043780395
6	1.0484740609855916	2.8133334108036188
7	0.8483935090750822	2.6558750559238624
8	0.6418168353492965	2.2962192564014665
9	0.5092959503176604	2.049863749135722
>10	2.9726253426704257	22.609509090169603
>50	0.03507905780249191	0.9662481334503135
>100	0.00649612181527628	0.6809638078194633
>500	0.0	0.0
>1k	0.0012992243630552559	2.476337669732553
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCGT	4262	2.476337669732553	No Hit
TCTTATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCG	416	0.2417072901475228	No Hit
CTTATACACATCTCCGAGCCCACGAGACGACTTAGTACCTCGTATGCCGT	265	0.15397219204109025	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCC	206	0.11969159079420601	No Hit
CTTATACACATCTCCGAGCCCACGAGACGACTTAGTAACTCGTATGCCGT	181	0.10516591229976352	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.001743081419333097	0.0
9	0.0	0.0	0.0	0.001743081419333097	0.0
10	0.0	0.0	0.0	0.001743081419333097	0.0
11	0.0	0.0	0.0	0.001743081419333097	0.0
12	0.0	0.0	0.0	0.001743081419333097	0.0
13	0.0	0.0	0.0	0.001743081419333097	0.0
14	0.0	0.0	0.0	0.002324108559110796	0.0
15	0.0	0.0	0.0	0.002324108559110796	0.0
16	0.0	0.0	0.0	0.002324108559110796	0.0
17	0.0	0.0	0.0	0.002324108559110796	0.0
18	0.0	0.0	0.0	0.002905135698888495	0.0
19	0.0	0.0	0.0	0.002905135698888495	0.0
20	0.0	0.0	0.0	0.002905135698888495	0.0
21	0.0	0.0	0.0	0.004067189978443893	0.0
22	0.0	0.0	0.0	0.004648217118221592	0.0
23	0.0	0.0	0.0	0.005229244257999291	0.0
24	0.0	0.0	0.0	0.005229244257999291	0.0
25	0.0	0.0	0.0	0.005229244257999291	0.0
26	0.0	0.0	0.0	0.008134379956887787	0.0
27	0.0	0.0	0.0	0.02324108559110796	0.0
28	0.0	0.0	0.0	0.04183395406399433	0.0
29	0.0	0.0	0.0	0.07611455531087857	0.0
30	0.0	0.0	0.0	0.10051769518154192	0.0
31	0.0	0.0	0.0	0.1225967264930945	0.0
32	0.0	0.0	0.0	0.16384965341731111	0.0
33	0.0	0.0	0.0	0.21904723169619253	0.0
34	0.0	0.0	0.0	0.3038771941037366	0.0
35	0.0	0.0	0.0	0.43925651767194046	0.0
36	0.0	0.0	0.0	0.6519124508305782	0.0
37	0.0	0.0	0.0	0.99994770755742	0.0
38	0.0	0.0	0.0	1.3944651354664777	0.0
39	0.0	0.0	0.0	1.8558006844499706	0.0
40	0.0	0.0	0.0	2.420559064313894	0.0
41	0.0	0.0	0.0	2.9917087427153723	0.0
42	0.0	0.0	0.0	3.608178538019511	0.0
43	0.0	0.0	0.0	4.405928800934292	0.0
44	0.0	0.0	0.0	5.248418153611955	0.0
45	0.0	0.0	0.0	6.154820491665165	0.0
46	0.0	0.0	0.0	7.151863063523697	0.0
47	0.0	0.0	0.0	8.167498503855114	0.0
48	0.0	0.0	0.0	9.08029214044588	0.0
49	0.0	0.0	0.0	9.980303179961536	0.0
50	0.0	0.0	0.0	10.855330052466751	0.0
51	0.0	0.0	0.0	11.671673183854418	0.0
52	0.0	0.0	0.0	12.481043989564753	0.0
53	0.0	0.0	0.0	13.303197392350196	0.0
54	0.0	0.0	0.0	14.163117559221192	0.0
55	0.0	0.0	0.0	15.086950711467733	0.0
56	0.0	0.0	0.0	15.979989425306057	0.0
57	0.0	0.0	0.0	16.834099320779274	0.0
58	0.0	0.0	0.0	17.698086677628712	0.0
59	0.0	0.0	0.0	18.52663137895171	0.0
60	0.0	0.0	0.0	19.32728677756538	0.0
61	0.0	0.0	0.0	20.116321633383496	0.0
62	0.0	0.0	0.0	20.870494860814947	0.0
63	0.0	0.0	0.0	21.60549419263374	0.0
64	0.0	0.0	0.0	22.383489532796077	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGCTCG	20	7.909507E-5	70.0	36
CGCTACA	25	2.3853096E-4	55.999996	63
CCGTCTT	615	0.0	55.203255	47
CGTCTTC	605	0.0	54.958683	48
TATGCCG	650	0.0	54.923077	43
CGTGCGA	45	2.685556E-8	54.444447	10
CTCGTAT	625	0.0	54.319996	39
CGTATGC	660	0.0	54.090908	41
TCGTATG	645	0.0	53.72093	40
TCTCGTA	580	0.0	53.706898	38
GTATCTC	575	0.0	53.565216	35
GCCGTCT	635	0.0	53.464565	46
ATGCCGT	660	0.0	53.030304	44
GTATGCC	675	0.0	52.88889	42
CTTGAAA	605	0.0	52.64463	57
CGGAATG	20	0.0069420477	52.5	4
GAATCCA	20	0.0069420477	52.5	34
CGAGAAT	40	7.446888E-7	52.5	14
ATCTCGT	600	0.0	51.916668	37
CTGCTTG	630	0.0	51.666668	54
>>END_MODULE