FastQCFastQC Report
Wed 25 May 2016
SRR1781215_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781215_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences270506
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGCCG200.0069463252.564
TACCACG200.0069463252.529
TACTGGC200.0069463252.57
TCGTTCC404.5854482E-543.7562
ACGTTAG404.5854482E-543.7570
GTAAGCG459.18325E-538.8888946
GGCCCGT501.7070342E-435.00000452
TATACCG501.7070342E-435.0000046
CCTGTGC601.213117E-535.08
AAATTCG400.00241617535.030
TAAGCGC652.0981144E-532.30769347
CTCGTTC552.9874506E-431.8181861
ATTAGAG552.9874506E-431.818183
CCACGTA552.9874506E-431.8181860
GCACCGA450.004302695431.11111310
TAACCGG450.004302695431.11111322
ATTATCG450.004302695431.11111348
ATTACAC604.974343E-429.1666663
ACTGTGC604.974343E-429.1666668
CACGTAA604.974343E-429.16666661