##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1781215_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	270506
Sequences flagged as poor quality	0
Sequence length	76
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.34377795686602	34.0	32.0	34.0	23.0	34.0
2	31.5343541363223	34.0	33.0	34.0	23.0	34.0
3	31.47590441616822	34.0	33.0	34.0	23.0	34.0
4	31.640248275454148	34.0	34.0	34.0	23.0	34.0
5	31.680502465749374	34.0	34.0	34.0	23.0	34.0
6	34.52903447612992	38.0	35.0	38.0	26.0	38.0
7	34.517851729721336	38.0	34.0	38.0	26.0	38.0
8	34.29897303571825	37.0	34.0	38.0	24.0	38.0
9	34.49764885067244	38.0	34.0	38.0	26.0	38.0
10-11	34.406698927195706	38.0	34.0	38.0	25.0	38.0
12-13	34.38142407192447	38.0	34.0	38.0	25.0	38.0
14-15	34.25936762955351	37.5	34.0	38.0	24.0	38.0
16-17	34.22366971527434	37.5	34.0	38.0	24.0	38.0
18-19	34.2925498879877	37.0	34.0	38.0	24.5	38.0
20-21	34.21184742667445	37.0	34.0	38.0	24.0	38.0
22-23	34.280496550908296	37.5	34.0	38.0	24.5	38.0
24-25	34.27876645989368	37.0	34.0	38.0	24.5	38.0
26-27	34.203359629730954	37.0	34.0	38.0	24.0	38.0
28-29	34.28987896756449	37.0	34.0	38.0	24.0	38.0
30-31	34.3643024553984	38.0	34.0	38.0	25.0	38.0
32-33	34.30672887107865	37.5	34.0	38.0	24.5	38.0
34-35	34.33360443021596	37.5	34.0	38.0	24.5	38.0
36-37	34.19580896542036	37.0	34.0	38.0	24.0	38.0
38-39	34.20367015888742	37.0	34.0	38.0	24.0	38.0
40-41	34.20585125653405	37.0	34.0	38.0	24.0	38.0
42-43	34.27736168513822	37.0	34.0	38.0	24.0	38.0
44-45	34.299743813445914	37.0	34.0	38.0	24.5	38.0
46-47	34.29947394882184	37.0	34.0	38.0	24.0	38.0
48-49	34.27100507936977	37.0	34.0	38.0	24.0	38.0
50-51	34.14738305250161	37.0	34.0	38.0	24.0	38.0
52-53	34.22109491101861	37.0	34.0	38.0	24.0	38.0
54-55	34.191193540993545	37.0	34.0	38.0	24.0	38.0
56-57	34.145129867729366	37.0	34.0	38.0	24.0	38.0
58-59	34.14278980872883	37.0	34.0	38.0	24.0	38.0
60-61	34.13976030106541	37.0	34.0	38.0	24.0	38.0
62-63	34.101740811664065	37.0	34.0	38.0	24.0	38.0
64-65	34.118975179848135	37.0	34.0	38.0	24.0	38.0
66-67	34.01406438304511	37.0	34.0	38.0	24.0	38.0
68-69	33.81674713315047	37.0	34.0	38.0	23.5	38.0
70-71	33.96579558309243	37.0	34.0	38.0	23.5	38.0
72-73	33.95872180284356	37.0	34.0	38.0	24.0	38.0
74-75	33.96728723207618	37.0	34.0	38.0	24.0	38.0
76	32.697574175803865	37.0	31.0	38.0	21.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	6.0
13	36.0
14	103.0
15	304.0
16	645.0
17	1195.0
18	1667.0
19	1900.0
20	1893.0
21	2127.0
22	2240.0
23	2563.0
24	3086.0
25	3795.0
26	4351.0
27	5119.0
28	6077.0
29	7120.0
30	8697.0
31	10470.0
32	12648.0
33	15488.0
34	19478.0
35	26138.0
36	40691.0
37	92669.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	47.858827530627785	18.229170517474657	9.336576637856462	24.575425314041095
2	23.900394076286663	20.6065669523042	31.13165696879182	24.36138200261732
3	19.54337426896261	22.50227351703844	30.0880571965132	27.866295017485747
4	13.945716546028553	16.86284222900786	37.69676088515597	31.49468033980762
5	13.62964222605044	33.92013485837652	36.38070874583189	16.069514169741154
6	29.251846539448294	37.95442614951239	18.775553961834486	14.018173349204824
7	28.73910375370602	29.84111258160632	22.612807109638972	18.80697655504869
8	25.546198605576215	34.758193903277565	20.103805460877023	19.591802030269196
9	24.811279601931197	16.400005914841074	21.694158355082696	37.09455612814504
10-11	25.083547130193047	24.80018927491442	28.12174221643882	21.99452137845371
12-13	25.245281065854364	22.910952067606633	27.087014705773626	24.75675216076538
14-15	23.332569333027735	24.209444522487487	24.348812965331636	28.109173179153142
16-17	23.562323941058608	27.474806473793556	25.39333693152832	23.56953265361951
18-19	22.90817948585244	27.033226619742262	26.707540683016273	23.351053211389026
20-21	22.960119184047674	27.03877178325065	26.630832587816904	23.370276444884773
22-23	23.35438030949406	26.37575506643106	26.938589162532438	23.331275461542443
24-25	23.000414038875295	26.640813882132004	26.857814614093588	23.500957464899113
26-27	22.127790141438638	27.152447635172607	27.082948252534138	23.636813970854618
28-29	22.692842302943372	26.714010040442727	27.803449831057353	22.789697825556548
30-31	23.125365056597637	26.48296156092656	27.29717640274153	23.094496979734274
32-33	22.57990580615587	27.03821726689981	27.007718867603675	23.374158059340644
34-35	23.371015800019222	26.213281775635288	27.3339593206805	23.081743103664984
36-37	23.471383259521044	26.247476950603684	26.828610086282744	23.452529703592525
38-39	22.658462289191366	26.952636910087023	27.535248756034985	22.853652044686623
40-41	22.908549163419664	26.140085617324573	27.825075968740066	23.1262892505157
42-43	22.587114518716774	26.24618307911839	27.567965220734475	23.59873718143036
44-45	22.133889821297863	26.28222664192291	27.71676044154289	23.867123095236334
46-47	22.29987504898228	26.124559159501082	27.86647985626936	23.709085935247277
48-49	21.861807131819628	26.08833075791295	28.457039769912683	23.592822340354743
50-51	21.936482000399252	26.074837526709203	28.018232497615582	23.970447975275963
52-53	21.585657989101907	25.882235514184526	28.681618892002398	23.85048760471117
54-55	21.22041655268275	26.347105054971053	28.715259550619948	23.717218841726247
56-57	21.096652557254025	26.26217629988355	28.606495258867675	24.03467588399475
58-59	21.412912885159237	26.17770466349975	28.266390639729394	24.142991811611616
60-61	21.20201105340012	26.02391822701983	28.186170311084823	24.587900408495223
62-63	20.633629692611965	25.839818117964548	28.83772943198832	24.688822757435165
64-65	21.411803848357703	26.035932792369827	28.077854383467958	24.47440897580451
66-67	21.5397127594684	25.704330788710006	27.882109387996522	24.873847063825067
68-69	21.04785493798636	25.963660560802943	27.680449529583555	25.30803497162714
70-71	21.638047355871425	25.872165024676068	27.46289347701521	25.026894142437296
72-73	21.593316204876064	25.533354281806254	27.451433430065986	25.421896083251696
74-75	21.91142492745051	25.619304633925438	27.468808339956745	25.00046209866731
76	22.225385019186266	25.344354653870894	27.073336635786266	25.356923691156574
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	1.5
8	3.0
9	2.0
10	1.0
11	1.0
12	1.0
13	2.5
14	15.5
15	32.0
16	61.0
17	85.0
18	104.0
19	179.0
20	294.5
21	354.0
22	416.5
23	610.5
24	898.0
25	1054.0
26	1231.5
27	1687.5
28	2169.0
29	2372.0
30	2805.0
31	3559.5
32	4689.5
33	5498.0
34	5841.0
35	7004.5
36	8709.5
37	9594.0
38	10142.0
39	12050.0
40	14309.0
41	15667.5
42	16127.0
43	16343.0
44	16849.5
45	16938.0
46	16736.0
47	16313.0
48	14956.0
49	13381.5
50	12741.0
51	11943.0
52	10075.0
53	8352.0
54	7699.0
55	7182.0
56	6160.5
57	5113.5
58	4571.0
59	3998.5
60	3155.0
61	2788.0
62	2692.0
63	2532.5
64	2266.0
65	2074.0
66	1962.5
67	1936.0
68	1902.5
69	1817.0
70	1758.5
71	1752.0
72	1719.0
73	1567.5
74	1385.5
75	1322.0
76	1246.0
77	1083.0
78	897.0
79	798.0
80	697.0
81	536.0
82	402.0
83	328.0
84	257.5
85	153.5
86	99.0
87	78.0
88	57.0
89	25.5
90	13.5
91	7.5
92	3.0
93	1.5
94	0.5
95	1.5
96	2.0
97	1.5
98	0.5
99	4.0
100	8.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	3.696775672258656E-4
58-59	3.696775672258656E-4
60-61	3.696775672258656E-4
62-63	3.696775672258656E-4
64-65	3.696775672258656E-4
66-67	3.696775672258656E-4
68-69	3.696775672258656E-4
70-71	3.696775672258656E-4
72-73	3.696775672258656E-4
74-75	3.696775672258656E-4
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	270506.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.22468311053652
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.39239911378058	36.35885305101114
2	14.025736193755067	14.088763114466618
3	5.366927865523222	8.086567539690359
4	2.2639442199163216	4.548235241009121
5	1.184450913798889	2.97443359027673
6	0.7334307123714379	2.2101795067434313
7	0.5563621160915575	1.95601776827842
8	0.4433206049920739	1.7812509521678592
9	0.3754162780648576	1.6969647240310082
>10	2.5805729107973194	23.779545143678302
>50	0.07448901106456791	2.3510211620059938
>100	0.002950059844141304	0.16816820664104634
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	3.696775672258656E-4	0.0
2	0.0	0.0	0.0	3.696775672258656E-4	0.0
3	0.0	0.0	0.0	3.696775672258656E-4	0.0
4	0.0	0.0	0.0	0.0011090327016775967	0.0
5	0.0	0.0	0.0	0.0011090327016775967	0.0
6	0.0	0.0	0.0	0.0011090327016775967	0.0
7	0.0	0.0	0.0	0.0011090327016775967	0.0
8	0.0	0.0	0.0	0.0011090327016775967	0.0
9	0.0	0.0	0.0	0.0011090327016775967	0.0
10	0.0	0.0	0.0	0.0014787102689034625	0.0
11	0.0	0.0	0.0	0.0014787102689034625	0.0
12	0.0	0.0	0.0	0.001848387836129328	0.0
13	0.0	0.0	0.0	0.001848387836129328	0.0
14	0.0	0.0	0.0	0.0022180654033551934	0.0
15	0.0	0.0	0.0	0.0022180654033551934	0.0
16	0.0	0.0	0.0	0.0022180654033551934	0.0
17	0.0	0.0	0.0	0.0022180654033551934	0.0
18	0.0	0.0	0.0	0.0022180654033551934	0.0
19	0.0	0.0	0.0	0.0022180654033551934	0.0
20	0.0	0.0	0.0	0.002587742970581059	0.0
21	0.0	0.0	0.0	0.002587742970581059	0.0
22	0.0	0.0	0.0	0.002587742970581059	0.0
23	0.0	0.0	0.0	0.003696775672258656	0.0
24	0.0	0.0	0.0	0.004066453239484522	0.0
25	0.0	0.0	0.0	0.005175485941162118	0.0
26	0.0	0.0	0.0	0.005545163508387984	0.0
27	0.0	0.0	0.0	0.012199359718453566	0.0
28	0.0	0.0	0.0	0.016635490525163953	0.0
29	0.0	0.0	0.0	0.032161948348650306	0.0
30	0.0	0.0	0.0	0.04805808373936253	0.0
31	0.0	0.0	0.0	0.06321486399562301	0.0
32	0.0	0.0	0.0	0.08650455073085254	0.0
33	0.0	0.0	0.0	0.11570907854169593	0.0
34	0.0	0.0	0.0	0.16080974174325155	0.0
35	0.0	0.0	0.0	0.2236549281716487	0.0
36	0.0	0.0	0.0	0.34638788049063607	0.0
37	0.0	0.0	0.0	0.5145911735784049	0.0
38	0.0	0.0	0.0	0.7171744804181792	0.0
39	0.0	0.0	0.0	0.9877784596275129	0.0
40	0.0	0.0	0.0	1.2672547004502672	0.0
41	0.0	0.0	0.0	1.5873954736678668	0.0
42	0.0	0.0	0.0	1.9319349663223737	0.0
43	0.0	0.0	0.0	2.345604164048117	0.0
44	0.0	0.0	0.0	2.8036346698409647	0.0
45	0.0	0.0	0.0	3.35667231041086	0.0
46	0.0	0.0	0.0	3.9104493061152064	0.0
47	0.0	0.0	0.0	4.534834717159693	0.0
48	0.0	0.0	0.0	5.119294950943787	0.0
49	0.0	0.0	0.0	5.7056035725640095	0.0
50	0.0	0.0	0.0	6.229436685323061	0.0
51	0.0	0.0	0.0	6.754748508351017	0.0
52	0.0	0.0	0.0	7.250116448433676	0.0
53	0.0	0.0	0.0	7.754726327696983	0.0
54	0.0	0.0	0.0	8.302588482325715	0.0
55	0.0	0.0	0.0	8.913295823382846	0.0
56	0.0	0.0	0.0	9.505519286078682	0.0
57	0.0	0.0	0.0	10.101809202014003	0.0
58	0.0	0.0	0.0	10.660761683659512	0.0
59	0.0	0.0	0.0	11.205666417750438	0.0
60	0.0	0.0	0.0	11.74465631076575	0.0
61	0.0	0.0	0.0	12.29177911026003	0.0
62	0.0	0.0	0.0	12.818199965989663	0.0
63	0.0	0.0	0.0	13.325027910656326	0.0
64	0.0	0.0	0.0	13.881022971764027	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTGCCG	20	0.00694632	52.5	64
TACCACG	20	0.00694632	52.5	29
TACTGGC	20	0.00694632	52.5	7
TCGTTCC	40	4.5854482E-5	43.75	62
ACGTTAG	40	4.5854482E-5	43.75	70
GTAAGCG	45	9.18325E-5	38.88889	46
GGCCCGT	50	1.7070342E-4	35.000004	52
TATACCG	50	1.7070342E-4	35.000004	6
CCTGTGC	60	1.213117E-5	35.0	8
AAATTCG	40	0.002416175	35.0	30
TAAGCGC	65	2.0981144E-5	32.307693	47
CTCGTTC	55	2.9874506E-4	31.81818	61
ATTAGAG	55	2.9874506E-4	31.81818	3
CCACGTA	55	2.9874506E-4	31.81818	60
GCACCGA	45	0.0043026954	31.111113	10
TAACCGG	45	0.0043026954	31.111113	22
ATTATCG	45	0.0043026954	31.111113	48
ATTACAC	60	4.974343E-4	29.166666	3
ACTGTGC	60	4.974343E-4	29.166666	8
CACGTAA	60	4.974343E-4	29.166666	61
>>END_MODULE