FastQCFastQC Report
Wed 25 May 2016
SRR1781215_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781215_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences243398
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCGT29611.216526019112729No Hit
TCTTATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCG3940.1618747894395188No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCC2540.10435582872496897No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT4800.054.687539
TATGCCG5000.054.643
TCGTATG5050.054.0594140
TCTCGTA4500.053.66666838
CGTATGC5100.053.5294141
CCGTCTT4950.053.030347
CGTCTTC5000.052.548
ATCTCGT4650.051.9354937
AATCTCG4650.051.9354936
CTGCTTG4950.051.61615854
GCCGTCT5100.051.4705946
GTATGCC5500.049.63636442
CTTGAAA5350.047.75700857
TGCCGTC5650.047.69911645
GTCTTCT5500.047.0909149
CTTCTGC5600.046.87551
ATGCCGT5750.046.86956444
TTCTGCT5850.046.06837552
TGCTTGA5550.046.03603755
TCTTCTG5700.045.438650
Produced by FastQC (version 0.11.5)