FastQCFastQC Report
Wed 25 May 2016
SRR1781214_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781214_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences171844
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTACC200.006942028652.49999614
TCTACGC200.006942028652.4999963
AATTGCC503.4635686E-642.00000429
ATTGCCC501.704446E-435.00000430
GAGACAT400.002413730134.99999625
ACTGTAG400.002413730134.9999968
GTGTTTC450.00429836731.11110945
GGCCAAG450.00429836731.11110933
AGCTCGG450.00429836731.11110943
TAGTATC450.00429836731.11110941
GTGCTGA604.9668754E-429.1666681
CTAGGGA604.9668754E-429.1666684
TAGAGCA500.007193576528.0000024
CACGAAA500.007193576528.00000229
GTAATCA500.007193576528.00000256
ACAAGTT500.007193576528.00000237
GGGTTAT657.9316634E-426.9230771
GTAGCTC700.001222311825.09
GGAGTAA750.001826721423.33333453
GGTGCTC750.001826721423.3333348
Produced by FastQC (version 0.11.5)