##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1781214_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	171844
Sequences flagged as poor quality	0
Sequence length	76
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.000785596238448	34.0	32.0	34.0	21.0	34.0
2	31.118462093526688	34.0	32.0	34.0	23.0	34.0
3	31.098251902888666	34.0	32.0	34.0	23.0	34.0
4	31.25423640045623	34.0	33.0	34.0	23.0	34.0
5	31.221031866111122	34.0	33.0	34.0	23.0	34.0
6	33.99477433020647	37.0	34.0	38.0	23.0	38.0
7	33.824317404157256	37.0	34.0	38.0	23.0	38.0
8	33.699890598449755	37.0	34.0	38.0	22.0	38.0
9	34.030341472498314	37.0	34.0	38.0	24.0	38.0
10-11	33.86223842554875	37.0	34.0	38.0	23.0	38.0
12-13	33.833401224366284	37.0	34.0	38.0	23.0	38.0
14-15	33.717432671492745	37.0	34.0	38.0	22.0	38.0
16-17	33.69348071506715	37.0	34.0	38.0	22.0	38.0
18-19	33.72556504736855	37.0	34.0	38.0	22.0	38.0
20-21	33.664774446591096	37.0	34.0	38.0	22.0	38.0
22-23	33.74222550685505	37.0	34.0	38.0	22.5	38.0
24-25	33.72905658620609	37.0	34.0	38.0	22.5	38.0
26-27	33.65916761714113	37.0	34.0	38.0	22.0	38.0
28-29	33.75240043295082	37.0	34.0	38.0	22.5	38.0
30-31	33.81706082260655	37.0	34.0	38.0	23.0	38.0
32-33	33.75375049463467	37.0	34.0	38.0	22.5	38.0
34-35	33.795087404855565	37.0	34.0	38.0	23.0	38.0
36-37	33.668664602779266	37.0	34.0	38.0	22.0	38.0
38-39	33.67960766741929	37.0	34.0	38.0	22.0	38.0
40-41	33.65626963990596	37.0	34.0	38.0	22.0	38.0
42-43	33.740988920183426	37.0	34.0	38.0	22.5	38.0
44-45	33.77895940504178	37.0	34.0	38.0	23.0	38.0
46-47	33.75183887712111	37.0	34.0	38.0	22.5	38.0
48-49	33.72640592630525	37.0	34.0	38.0	22.5	38.0
50-51	33.605589953678916	37.0	34.0	38.0	22.0	38.0
52-53	33.67127743767603	37.0	34.0	38.0	22.0	38.0
54-55	33.62408638067084	37.0	34.0	38.0	22.0	38.0
56-57	33.59801040478574	37.0	34.0	38.0	22.0	38.0
58-59	33.57965945857871	37.0	34.0	38.0	22.0	38.0
60-61	33.56697062451991	37.0	34.0	38.0	22.0	38.0
62-63	33.544770256744485	37.0	34.0	38.0	21.5	38.0
64-65	33.58175438188124	37.0	34.0	38.0	22.0	38.0
66-67	33.45875910709714	37.0	34.0	38.0	21.5	38.0
68-69	33.24762575359047	37.0	33.5	38.0	21.0	38.0
70-71	33.381305137217474	37.0	34.0	38.0	21.0	38.0
72-73	33.37665557133214	37.0	33.5	38.0	21.0	38.0
74-75	33.405454947510535	37.0	33.0	38.0	21.5	38.0
76	32.143112357719794	37.0	31.0	38.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	2.0
13	34.0
14	147.0
15	382.0
16	775.0
17	1414.0
18	1650.0
19	1795.0
20	1710.0
21	1772.0
22	1823.0
23	2005.0
24	2314.0
25	2646.0
26	2998.0
27	3609.0
28	4078.0
29	4773.0
30	5569.0
31	6651.0
32	8010.0
33	9902.0
34	12507.0
35	16335.0
36	24969.0
37	53972.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.25932822792766	20.06121831428505	9.625590651986686	26.05386280580061
2	21.11275342752729	19.695770582621446	34.46439794231978	24.72707804753148
3	19.58927864807616	22.65601359372454	29.250366611577945	28.504341146621353
4	13.682176858080586	15.945857871092386	37.315821326319224	33.05614394450781
5	13.543679150857754	34.970089150624986	36.007076185377436	15.479155513139824
6	30.996717953492702	37.53637019622448	16.969460673634227	14.497451176648587
7	28.61432462000419	28.688810781871933	22.300458555434	20.396406042689883
8	24.963338842205722	35.638718838015876	19.281441307232143	20.116501012546262
9	24.460557249598473	14.184376527548242	21.68245618118759	39.6726100416657
10-11	25.32122157305463	24.64357207700007	27.79526780102884	22.23993854891646
12-13	24.68605246619027	22.76221456670003	27.650077977700704	24.901654989409
14-15	22.900421312353064	24.3319522357487	24.53737110402458	28.23025534787365
16-17	23.39563790414562	27.295686785689345	25.328495612299527	23.980179697865506
18-19	22.826808035194713	26.79785153976863	26.64567863876539	23.72966178627127
20-21	22.90129419706245	26.89532366565024	26.665173063941715	23.53820907334559
22-23	23.06394171457834	26.287795907916482	26.918309629664115	23.729952747841065
24-25	22.987709783291823	26.717837108074765	26.356462838388307	23.937990270245106
26-27	22.35283163799725	26.743732687786597	27.078047531482042	23.82538814273411
28-29	22.794220339377574	26.52929401084705	27.584611624496635	23.09187402527874
30-31	23.08925537115058	26.654116524289474	26.72394730104048	23.532680803519472
32-33	22.54573915877191	26.770501152207814	27.156025232187332	23.527734456832942
34-35	23.39447405786644	26.04106049672959	27.22381927794977	23.340646167454203
36-37	23.21844230813994	26.206908591513233	26.7646819208119	23.809967179534926
38-39	22.34032029049603	26.596506133469894	27.78799376178394	23.275179814250134
40-41	22.90769535159796	26.0334954959149	27.805451455971696	23.253357696515444
42-43	22.65601359372454	26.014582993878165	27.548532389841952	23.780871022555342
44-45	22.086892763203835	26.00090780009776	27.8613160773725	24.0508833593259
46-47	22.100276995414443	25.967738181141037	27.935511277670443	23.996473545774073
48-49	21.802914271083075	26.04600684341612	28.452840948767488	23.698237936733317
50-51	21.73570214846023	26.15569935522916	28.037056865529202	24.071541630781407
52-53	21.6746002188031	26.00410837736552	28.49328460696911	23.82800679686227
54-55	21.292567677661133	26.352098414841368	28.112415912106332	24.24291799539117
56-57	21.149414585321573	26.358790530946674	28.235201694560182	24.256593189171575
58-59	21.373454994064385	26.023311840972045	28.212506692116108	24.390726472847465
60-61	21.466271734829263	25.79636181653127	28.215416307814063	24.5219501408254
62-63	20.971346104606504	25.971229719978584	28.42956402318382	24.627860152231094
64-65	21.560252321873328	26.04106049672959	27.8543329996974	24.54435418169968
66-67	21.429028653895394	26.06666511487163	27.516235655594606	24.988070575638368
68-69	21.397022881217847	26.00905472405205	27.45920718791462	25.134715206815482
70-71	21.793603500849606	25.936605293172875	27.66666278717907	24.603128418798445
72-73	21.446777309652944	25.69452526710272	27.522054886990528	25.336642536253812
74-75	21.980691790228345	25.84698912967575	27.438548916459116	24.733770163636787
76	22.150904308558925	25.63778776099253	27.122855613230605	25.08845231721794
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	5.0
14	17.5
15	33.5
16	72.5
17	103.0
18	130.5
19	200.5
20	271.0
21	299.0
22	389.0
23	575.5
24	775.5
25	879.0
26	1073.0
27	1342.0
28	1669.0
29	1921.0
30	2120.5
31	2609.0
32	3346.0
33	3794.0
34	4094.0
35	4791.0
36	5808.0
37	6428.0
38	6668.0
39	7496.5
40	8612.5
41	9369.0
42	9598.0
43	9726.5
44	9737.5
45	9601.0
46	9582.0
47	9403.5
48	8676.0
49	7758.0
50	7389.0
51	6923.5
52	5830.5
53	5071.5
54	4940.0
55	4566.5
56	3912.0
57	3352.0
58	3073.0
59	2774.5
60	2296.5
61	1974.0
62	1831.0
63	1735.0
64	1675.0
65	1612.5
66	1488.5
67	1463.0
68	1500.0
69	1448.5
70	1337.0
71	1314.0
72	1258.0
73	1211.5
74	1157.0
75	1093.0
76	1050.5
77	904.0
78	669.0
79	538.0
80	491.0
81	401.5
82	302.0
83	245.0
84	205.0
85	135.5
86	78.5
87	51.0
88	42.5
89	28.5
90	18.5
91	10.0
92	6.0
93	3.5
94	1.5
95	2.0
96	2.0
97	1.0
98	0.5
99	2.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	171844.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.31880798862282
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.01266880629971	43.90343289646428
2	14.402935549391795	17.087299366537636
3	5.3434158929410565	9.508951840701782
4	2.1009981186793403	4.985148159455903
5	0.9899957240257021	2.936268313151913
6	0.614997252156767	2.1888542348530753
7	0.442998017791948	1.8394680049718755
8	0.3419984696212576	1.6229553241492989
9	0.2989986620336416	1.5962619799825841
>10	1.4479935204831906	14.218654648889398
>50	0.0029999865755867103	0.1127052308422603
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	5.819231395917228E-4	0.0
6	0.0	0.0	0.0	5.819231395917228E-4	0.0
7	0.0	0.0	0.0	5.819231395917228E-4	0.0
8	0.0	0.0	0.0	0.001745769418775168	0.0
9	0.0	0.0	0.0	0.002327692558366891	0.0
10	0.0	0.0	0.0	0.003491538837550336	0.0
11	0.0	0.0	0.0	0.003491538837550336	0.0
12	0.0	0.0	0.0	0.003491538837550336	0.0
13	0.0	0.0	0.0	0.004073461977142059	0.0
14	0.0	0.0	0.0	0.004655385116733782	0.0
15	0.0	0.0	0.0	0.004655385116733782	5.819231395917228E-4
16	0.0	0.0	0.0	0.004655385116733782	5.819231395917228E-4
17	0.0	0.0	0.0	0.005819231395917227	5.819231395917228E-4
18	0.0	0.0	0.0	0.008146923954284117	5.819231395917228E-4
19	0.0	0.0	0.0	0.00872884709387584	5.819231395917228E-4
20	0.0	0.0	0.0	0.009310770233467564	5.819231395917228E-4
21	0.0	0.0	0.0	0.009892693373059286	5.819231395917228E-4
22	0.0	0.0	0.0	0.012220385931426178	5.819231395917228E-4
23	0.0	0.0	0.0	0.0128023090710179	5.819231395917228E-4
24	0.0	0.0	0.0	0.01745769418775168	5.819231395917228E-4
25	0.0	0.0	0.0	0.01745769418775168	5.819231395917228E-4
26	0.0	0.0	0.0	0.01745769418775168	5.819231395917228E-4
27	0.0	0.0	0.0	0.022113079304485462	5.819231395917228E-4
28	0.0	0.0	0.0	0.03200577267754475	5.819231395917228E-4
29	0.0	0.0	0.0	0.044226158608970924	5.819231395917228E-4
30	0.0	0.0	0.0	0.06284769907590605	5.819231395917228E-4
31	0.0	0.0	0.0	0.08903424035753357	5.819231395917228E-4
32	0.0	0.0	0.0	0.11056539652242732	5.819231395917228E-4
33	0.0	0.0	0.0	0.14431693861874723	5.819231395917228E-4
34	0.0	0.0	0.0	0.1995996368799609	5.819231395917228E-4
35	0.0	0.0	0.0	0.2787411838644352	5.819231395917228E-4
36	0.0	0.0	0.0	0.39570773492237143	5.819231395917228E-4
37	0.0	0.0	0.0	0.5830869858709061	5.819231395917228E-4
38	0.0	0.0	0.0	0.7972347012406601	5.819231395917228E-4
39	0.0	0.0	0.0	1.0503712669630596	5.819231395917228E-4
40	0.0	0.0	0.0	1.3139824491981098	5.819231395917228E-4
41	0.0	0.0	0.0	1.5793394008519355	5.819231395917228E-4
42	0.0	0.0	0.0	1.8883405879751403	5.819231395917228E-4
43	0.0	0.0	0.0	2.269500244407719	5.819231395917228E-4
44	0.0	0.0	0.0	2.694886059449268	5.819231395917228E-4
45	0.0	0.0	0.0	3.154605339726729	5.819231395917228E-4
46	0.0	0.0	0.0	3.6672796257070366	5.819231395917228E-4
47	0.0	0.0	0.0	4.149693908428575	5.819231395917228E-4
48	0.0	0.0	0.0	4.642582807662764	5.819231395917228E-4
49	0.0	0.0	0.0	5.1069574730569585	5.819231395917228E-4
50	0.0	0.0	0.0	5.513721747631573	5.819231395917228E-4
51	0.0	0.0	0.0	5.924559484183329	5.819231395917228E-4
52	0.0	0.0	0.0	6.353436838062429	5.819231395917228E-4
53	0.0	0.0	0.0	6.793952654733363	5.819231395917228E-4
54	0.0	0.0	0.0	7.233304625125114	5.819231395917228E-4
55	0.0	0.0	0.0	7.761108912734806	0.0011638462791834455
56	0.0	0.0	0.0	8.258071273946138	0.0011638462791834455
57	0.0	0.0	0.0	8.710807476548498	0.0011638462791834455
58	0.0	0.0	0.0	9.168780987407184	0.0011638462791834455
59	0.0	0.0	0.0	9.611624496636484	0.0011638462791834455
60	0.0	0.0	0.0	10.061451083540886	0.0011638462791834455
61	0.0	0.0	0.0	10.492656129978352	0.0011638462791834455
62	0.0	0.0	0.0	10.945392332580713	0.0011638462791834455
63	0.0	0.0	0.0	11.37310584018063	0.0011638462791834455
64	0.0	0.0	0.0	11.774632806498918	0.0011638462791834455
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTACC	20	0.0069420286	52.499996	14
TCTACGC	20	0.0069420286	52.499996	3
AATTGCC	50	3.4635686E-6	42.000004	29
ATTGCCC	50	1.704446E-4	35.000004	30
GAGACAT	40	0.0024137301	34.999996	25
ACTGTAG	40	0.0024137301	34.999996	8
GTGTTTC	45	0.004298367	31.111109	45
GGCCAAG	45	0.004298367	31.111109	33
AGCTCGG	45	0.004298367	31.111109	43
TAGTATC	45	0.004298367	31.111109	41
GTGCTGA	60	4.9668754E-4	29.166668	1
CTAGGGA	60	4.9668754E-4	29.166668	4
TAGAGCA	50	0.0071935765	28.000002	4
CACGAAA	50	0.0071935765	28.000002	29
GTAATCA	50	0.0071935765	28.000002	56
ACAAGTT	50	0.0071935765	28.000002	37
GGGTTAT	65	7.9316634E-4	26.923077	1
GTAGCTC	70	0.0012223118	25.0	9
GGAGTAA	75	0.0018267214	23.333334	53
GGTGCTC	75	0.0018267214	23.333334	8
>>END_MODULE