FastQCFastQC Report
Wed 25 May 2016
SRR1781214_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781214_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences155721
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCGT38232.4550317555114596No Hit
TCTTATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCG4280.2748505339678014No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCC2750.17659788981576025No Hit
CTTATACACATCTCCGAGCCCACGAGACTGTGCCTTACCTCGTATGCCGT2490.15990136205136107No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCTAT252.3846001E-456.00000419
TCTCGTA4900.055.038
CTCGTAT5500.054.09090839
CGTATGC5700.054.03508441
CCGTCTT5450.053.30275347
TATGCCG5650.053.27433443
ATCTCGT5150.053.0097137
CGTCTTC5350.052.99065448
TCGTATG5850.052.6495840
GTTCGCA200.006940810552.535
GCTACGG200.006940810552.535
TAAGCCG200.006940810552.545
AATCGGA200.006940810552.517
ATGCCGT5700.052.1929844
GCCGTCT5650.051.41592446
TGCCGTC5800.051.293145
GTCTTCT5550.051.08107849
GCCTTAT5300.050.84905232
GTATGCC6050.049.75206842
CTTATCT5250.049.33333234
Produced by FastQC (version 0.11.5)