FastQCFastQC Report
Wed 25 May 2016
SRR1781213_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781213_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences167530
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTTAGC150.002221578770.01
GTCTATG200.006941725552.52
GATCTAG200.006941725552.522
CGGTAAG200.006941725552.528
TCGAAAT305.8649515E-446.66666470
CGTTCGG350.001252700340.024
TTGCGTT350.001252700340.033
TACGTAC350.001252700340.041
AGCGGTC459.168274E-538.8888912
TACTGGC459.168274E-538.888897
CGGTCAA501.7042641E-435.00000414
TGATACT400.002413557635.04
CACCTTG400.002413557635.012
ATACTGG601.2103641E-535.06
GCATTAG400.002413557635.034
GAGCGGT552.9826272E-431.8181811
CGTTCAG450.00429806231.11111336
CTAGGGA450.00429806231.1111134
CGCCGAA604.966351E-429.16666666
TAGCGGC500.007193070428.0000027
Produced by FastQC (version 0.11.5)