##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1781213_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	167530
Sequences flagged as poor quality	0
Sequence length	76
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.16394675580493	34.0	32.0	34.0	21.0	34.0
2	31.314063152868144	34.0	33.0	34.0	23.0	34.0
3	31.23790365904614	34.0	33.0	34.0	23.0	34.0
4	31.410105652718915	34.0	33.0	34.0	23.0	34.0
5	31.433020951471377	34.0	33.0	34.0	23.0	34.0
6	34.21419447263177	37.0	34.0	38.0	24.0	38.0
7	34.17906643586223	37.0	34.0	38.0	24.0	38.0
8	34.04294156270519	37.0	34.0	38.0	24.0	38.0
9	34.26687757416582	37.0	34.0	38.0	24.0	38.0
10-11	34.13233749179251	37.0	34.0	38.0	24.0	38.0
12-13	34.07192144690503	37.0	34.0	38.0	23.0	38.0
14-15	33.93544141347819	37.0	34.0	38.0	23.0	38.0
16-17	33.894383095564976	37.0	34.0	38.0	23.0	38.0
18-19	33.96414970453053	37.0	34.0	38.0	23.0	38.0
20-21	33.892747567599834	37.0	34.0	38.0	23.0	38.0
22-23	33.94869874052408	37.0	34.0	38.0	23.0	38.0
24-25	33.940243538470725	37.0	34.0	38.0	23.0	38.0
26-27	33.84517101414672	37.0	34.0	38.0	23.0	38.0
28-29	33.95063272249746	37.0	34.0	38.0	23.0	38.0
30-31	34.002163791559724	37.0	34.0	38.0	23.0	38.0
32-33	33.95009550528263	37.0	34.0	38.0	23.0	38.0
34-35	33.97430310989077	37.0	34.0	38.0	23.0	38.0
36-37	33.82217811735212	37.0	34.0	38.0	22.5	38.0
38-39	33.84490837461947	37.0	34.0	38.0	23.0	38.0
40-41	33.842258103026325	37.0	34.0	38.0	23.0	38.0
42-43	33.92237808153763	37.0	34.0	38.0	23.0	38.0
44-45	33.95173998686802	37.0	34.0	38.0	23.0	38.0
46-47	33.922491494060765	37.0	34.0	38.0	23.0	38.0
48-49	33.884202829344	37.0	34.0	38.0	23.0	38.0
50-51	33.77048289858533	37.0	34.0	38.0	22.5	38.0
52-53	33.88105115501701	37.0	34.0	38.0	23.0	38.0
54-55	33.82459857935892	37.0	34.0	38.0	23.0	38.0
56-57	33.80439921208142	37.0	34.0	38.0	23.0	38.0
58-59	33.79841819375635	37.0	34.0	38.0	23.0	38.0
60-61	33.776732525517815	37.0	34.0	38.0	23.0	38.0
62-63	33.73442666985018	37.0	34.0	38.0	22.5	38.0
64-65	33.74359517698323	37.0	34.0	38.0	23.0	38.0
66-67	33.65728526234108	37.0	34.0	38.0	22.0	38.0
68-69	33.42405240852385	37.0	34.0	38.0	21.5	38.0
70-71	33.56499134483376	37.0	34.0	38.0	22.0	38.0
72-73	33.50916253805289	37.0	34.0	38.0	22.0	38.0
74-75	33.51339760042977	37.0	34.0	38.0	22.0	38.0
76	32.132728466543306	36.0	31.0	38.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	6.0
13	31.0
14	110.0
15	330.0
16	824.0
17	1331.0
18	1724.0
19	1790.0
20	1620.0
21	1452.0
22	1476.0
23	1613.0
24	1818.0
25	2230.0
26	2559.0
27	3083.0
28	3750.0
29	4389.0
30	5364.0
31	6469.0
32	7898.0
33	9831.0
34	12248.0
35	16694.0
36	24935.0
37	53954.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.05163254342506	18.883782009192384	10.26502715931475	25.799558288067807
2	22.706380946696115	20.010147436280068	32.00382021130544	25.27965140571838
3	19.662746970691817	22.110069838237926	29.19715871784158	29.03002447322868
4	14.07747866053841	16.413776637020234	37.208858114964485	32.29988658747687
5	13.625619292067093	34.50426789231779	35.988778129290274	15.881334686324838
6	30.19459201337074	36.93845878350146	18.048110786127857	14.818838416999942
7	29.12194830776577	28.72201993672775	22.484928072584015	19.67110368292246
8	25.90640482301677	33.454306691338864	19.856145168029606	20.783143317614755
9	24.564555601981734	15.357249447860086	21.839670506774905	38.23852444338328
10-11	25.37664895839551	24.281621202172747	27.907240494239836	22.434489345191906
12-13	25.256371993075867	22.829343998089893	26.374679161941145	25.539604846893095
14-15	23.40565868799618	24.004357428520265	23.966155315465887	28.623828568017668
16-17	23.762609681848026	26.462126186354684	25.447084104339524	24.32818002745777
18-19	23.16779084343103	26.103086014445175	26.58986450188026	24.13925864024354
20-21	23.241807437473884	26.431683877514473	26.39527248850952	23.93123619650212
22-23	23.407449412045604	25.746433474601567	26.826836984420698	24.019280128932134
24-25	23.172864561571064	26.07144988957202	26.460932370321732	24.29475317853519
26-27	22.62639527248851	26.22097534769892	26.652241389601862	24.500387990210708
28-29	23.115262937981257	26.08816331403331	27.443442965439026	23.353130782546412
30-31	23.443263893034082	25.87566406016833	26.728048707694146	23.953023339103442
32-33	22.8397898883782	26.54927475675998	26.518533993911536	24.092401360950277
34-35	23.842296902047394	25.474541873097355	26.912194830776574	23.77096639407867
36-37	23.604429057482243	25.35545872381066	26.638512505222945	24.401599713484153
38-39	23.00095505282636	25.989076583298516	27.20945502298096	23.80051334089417
40-41	23.310153405360236	24.80779561869516	27.91141885035516	23.970632125589447
42-43	22.54909568435504	25.308601444517397	27.6407210648839	24.501581806243657
44-45	22.17095445591834	25.087148570405297	27.74816450784934	24.993732465827016
46-47	22.61863546827434	24.72154241031457	27.95767922163195	24.702142899779144
48-49	21.870411269623354	24.60066853697845	28.95869396525995	24.570226228138246
50-51	21.72655643765296	24.62693248970334	28.454306691338864	25.19220438130484
52-53	21.33707395690324	24.589327284665433	29.05121470781353	25.022384050617802
54-55	20.665850892377485	25.237867844565155	28.916015042082016	25.18026622097535
56-57	20.47675327853685	25.21563430808994	28.807251281867618	25.50036113150559
58-59	20.8050546472551	25.005819887900003	28.427913973103163	25.761211491741726
60-61	20.505703490142004	24.54769025064317	28.75561843024193	26.190987828972894
62-63	20.187549618275042	24.45636277898155	29.21732953697569	26.13875806576772
64-65	21.07217257907586	24.599920013848347	28.36881972673388	25.959087680341913
66-67	21.09276602856819	24.45785505793027	27.610742020784464	26.838636892717084
68-69	20.78028281670636	24.84256457091011	27.19379928251228	27.183353329871245
70-71	21.460165105742888	24.699007336043312	27.120976069814777	26.719851488399023
72-73	21.46822341206597	24.002709978570874	27.08008762661987	27.448978982743288
74-75	21.63051880415091	24.363768769082462	27.037626209115412	26.968086217651223
76	22.065301737002326	24.09777353309855	26.489583955112515	27.347340774786606
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	1.0
12	2.0
13	3.5
14	10.0
15	20.0
16	29.0
17	33.0
18	38.0
19	58.0
20	90.5
21	108.0
22	139.0
23	254.0
24	379.5
25	421.0
26	560.0
27	735.5
28	929.5
29	1087.0
30	1315.0
31	1714.5
32	2326.5
33	2767.0
34	3068.5
35	3719.0
36	4811.0
37	5554.0
38	6047.5
39	7216.0
40	8523.5
41	9623.5
42	10091.0
43	10192.0
44	10408.0
45	10652.5
46	10782.0
47	10500.5
48	9645.5
49	8703.5
50	8335.0
51	7743.5
52	6462.0
53	5284.0
54	4796.0
55	4510.0
56	3779.0
57	2975.5
58	2617.0
59	2422.5
60	2070.5
61	1882.0
62	1851.0
63	1698.0
64	1560.5
65	1606.0
66	1618.5
67	1601.0
68	1525.0
69	1430.5
70	1362.5
71	1313.0
72	1271.0
73	1150.0
74	1083.0
75	1095.0
76	1015.5
77	869.0
78	715.0
79	628.0
80	548.5
81	403.0
82	301.5
83	266.0
84	223.5
85	133.0
86	68.0
87	51.0
88	42.0
89	25.0
90	15.5
91	10.0
92	6.0
93	4.0
94	1.0
95	0.5
96	1.0
97	0.5
98	0.5
99	3.0
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	5.969080164746613E-4
58-59	5.969080164746613E-4
60-61	5.969080164746613E-4
62-63	5.969080164746613E-4
64-65	5.969080164746613E-4
66-67	5.969080164746613E-4
68-69	5.969080164746613E-4
70-71	5.969080164746613E-4
72-73	5.969080164746613E-4
74-75	2.9845400823733066E-4
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	167530.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.04440995642572
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.31104484459226	37.633856622694445
2	13.499254501663035	14.051214707813525
3	5.422640211033376	8.466543305676595
4	2.439499942653974	5.0784934041664185
5	1.4439729326757655	3.757535963707993
6	0.8854226402110335	2.764877932310631
7	0.6663608211950912	2.4276249030024473
8	0.509232710173185	2.120217274517997
9	0.40830370455327447	1.9124932847848146
>10	2.390182360362427	21.041604488748284
>50	0.02408533088656956	0.745538112576852
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0011938160329493224	0.0
2	0.0	0.0	0.0	0.0017907240494239838	0.0
3	0.0	0.0	0.0	0.0017907240494239838	0.0
4	0.0	0.0	0.0	0.0035814480988479677	0.0
5	0.0	0.0	0.0	0.0035814480988479677	0.0
6	0.0	0.0	0.0	0.0035814480988479677	0.0
7	0.0	0.0	0.0	0.0035814480988479677	0.0
8	0.0	0.0	0.0	0.0035814480988479677	0.0
9	0.0	0.0	0.0	0.005372172148271952	0.0
10	0.0	0.0	0.0	0.005969080164746613	0.0
11	0.0	0.0	0.0	0.005969080164746613	0.0
12	0.0	0.0	0.0	0.005969080164746613	0.0
13	0.0	0.0	0.0	0.006565988181221274	0.0
14	0.0	0.0	0.0	0.007162896197695935	0.0
15	0.0	0.0	0.0	0.0077598042141705965	0.0
16	0.0	0.0	0.0	0.0077598042141705965	0.0
17	0.0	0.0	0.0	0.008356712230645257	0.0
18	0.0	0.0	0.0	0.008953620247119919	0.0
19	0.0	0.0	0.0	0.010147436280069241	0.0
20	0.0	0.0	0.0	0.010744344296543903	0.0
21	0.0	0.0	0.0	0.013131976362442548	0.0
22	0.0	0.0	0.0	0.016116516444815855	0.0
23	0.0	0.0	0.0	0.017310332477765176	0.0
24	0.0	0.0	0.0	0.01969796454366382	0.0
25	0.0	0.0	0.0	0.01969796454366382	0.0
26	0.0	0.0	0.0	0.01969796454366382	0.0
27	0.0	0.0	0.0	0.027457768757834417	0.0
28	0.0	0.0	0.0	0.04476810123559959	0.0
29	0.0	0.0	0.0	0.06207843371336477	0.0
30	0.0	0.0	0.0	0.08117949024055393	0.0
31	0.0	0.0	0.0	0.0984898227183191	0.0
32	0.0	0.0	0.0	0.1265444994926282	0.0
33	0.0	0.0	0.0	0.1725064167611771	0.0
34	0.0	0.0	0.0	0.2530889989852564	0.0
35	0.0	0.0	0.0	0.3497880976541515	0.0
36	0.0	0.0	0.0	0.5109532621023101	0.0
37	0.0	0.0	0.0	0.7915000298454008	0.0
38	0.0	0.0	0.0	1.1532262878290456	0.0
39	0.0	0.0	0.0	1.5352474183728289	0.0
40	0.0	0.0	0.0	1.9680057303169582	0.0
41	0.0	0.0	0.0	2.428221811018922	0.0
42	0.0	0.0	0.0	2.966035933862592	0.0
43	0.0	0.0	0.0	3.611890407688175	0.0
44	0.0	0.0	0.0	4.337730555721364	0.0
45	0.0	0.0	0.0	5.248015280845221	0.0
46	0.0	0.0	0.0	6.215006267534173	0.0
47	0.0	0.0	0.0	7.2303468035575715	0.0
48	0.0	0.0	0.0	8.174655285620485	0.0
49	0.0	0.0	0.0	9.07538948248075	0.0
50	0.0	0.0	0.0	9.897331821166357	0.0
51	0.0	0.0	0.0	10.6619709902704	0.0
52	0.0	0.0	0.0	11.427207067390915	0.0
53	0.0	0.0	0.0	12.269444278636662	0.0
54	0.0	0.0	0.0	13.078254640959829	0.0
55	0.0	0.0	0.0	14.044648719632304	0.0
56	0.0	0.0	0.0	15.011639706321256	0.0
57	0.0	0.0	0.0	15.97624306094431	0.0
58	0.0	0.0	0.0	16.858473109293858	0.0
59	0.0	0.0	0.0	17.72578045723154	0.0
60	0.0	0.0	0.0	18.583537276905627	0.0
61	0.0	0.0	0.0	19.38697546708052	0.0
62	0.0	0.0	0.0	20.079985674207606	0.0
63	0.0	0.0	0.0	20.855966095624666	0.0
64	0.0	0.0	0.0	21.66955172207963	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTTTAGC	15	0.0022215787	70.0	1
GTCTATG	20	0.0069417255	52.5	2
GATCTAG	20	0.0069417255	52.5	22
CGGTAAG	20	0.0069417255	52.5	28
TCGAAAT	30	5.8649515E-4	46.666664	70
CGTTCGG	35	0.0012527003	40.0	24
TTGCGTT	35	0.0012527003	40.0	33
TACGTAC	35	0.0012527003	40.0	41
AGCGGTC	45	9.168274E-5	38.88889	12
TACTGGC	45	9.168274E-5	38.88889	7
CGGTCAA	50	1.7042641E-4	35.000004	14
TGATACT	40	0.0024135576	35.0	4
CACCTTG	40	0.0024135576	35.0	12
ATACTGG	60	1.2103641E-5	35.0	6
GCATTAG	40	0.0024135576	35.0	34
GAGCGGT	55	2.9826272E-4	31.81818	11
CGTTCAG	45	0.004298062	31.111113	36
CTAGGGA	45	0.004298062	31.111113	4
CGCCGAA	60	4.966351E-4	29.166666	66
TAGCGGC	50	0.0071930704	28.000002	7
>>END_MODULE