FastQCFastQC Report
Wed 25 May 2016
SRR1781213_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781213_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences151354
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCGT44622.948055551884985No Hit
TCTTATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCG4560.3012804418779814No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCC2910.19226449251423813No Hit
CTTATACACATCTCCGAGCCCACGAGACCAATCTTAACCTCGTATGCCGT2600.17178270808832274No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTAAGC207.906429E-570.033
CAATACG150.002221163470.069
CTCGTAA308.345132E-658.33333230
CGTAAGA308.345132E-658.33333232
TCTTAAG252.3843863E-456.00000432
GGTTATC252.3843863E-456.0000047
TCTCGTA5700.055.2631638
CTCGTAT6800.055.07352439
GTATGCC7100.054.7183142
ATCTCGT5700.054.64912437
CGTATGC7050.054.6099341
TCGTATG7050.054.6099340
TATGCCG7200.054.44444743
CCGTCTT6950.054.3884947
AATCTCG5750.054.17391236
GTCTTCT7050.054.11347249
CGTCTTC7000.054.00000448
TCTTCTG7100.053.73239550
GCCGTCT7250.053.1034546
ATGCCGT7350.052.8571444