FastQCFastQC Report
Wed 25 May 2016
SRR1781212_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781212_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences245933
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTCTA200.006945572352.51
CACTTCG200.006945572352.567
GTTTGCG352.0826466E-550.038
TACTCGC305.870341E-446.66666820
GCGGTTT404.5842247E-543.7542
CGCGGTA350.001253845240.043
TAACGGC350.001253845240.036
TATCCAC459.180815E-538.88888565
ACCACGT556.6777593E-638.1818236
AACTCTC556.6777593E-638.1818219
GTAACCA556.6777593E-638.1818233
ACGTTCT601.2126682E-535.00000439
AGTAACC601.2126682E-535.00000432
GTGCTAG708.6629916E-735.027
ACTCGCA400.002415749235.021
TTTGCGG501.7065837E-435.039
GGTCTTT501.7065837E-435.058
TTAACGG552.9866648E-431.81818435
TAGATGG552.9866648E-431.81818432
ATTAGCG450.004301942431.1111098