##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1781212_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	245933
Sequences flagged as poor quality	0
Sequence length	76
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.647310446341077	34.0	33.0	34.0	24.0	34.0
2	31.712572123301875	34.0	34.0	34.0	24.0	34.0
3	31.615505035924418	34.0	34.0	34.0	23.0	34.0
4	31.80165329581634	34.0	34.0	34.0	23.0	34.0
5	31.848466858859933	34.0	34.0	34.0	27.0	34.0
6	34.80914720675956	38.0	35.0	38.0	27.0	38.0
7	34.63772246912777	38.0	35.0	38.0	26.0	38.0
8	34.417821113880606	37.0	34.0	38.0	25.0	38.0
9	34.75002134727751	38.0	35.0	38.0	27.0	38.0
10-11	34.61132706875449	38.0	35.0	38.0	26.0	38.0
12-13	34.56772169655963	38.0	35.0	38.0	26.0	38.0
14-15	34.457506719309734	38.0	34.0	38.0	25.5	38.0
16-17	34.44759141717459	37.5	34.0	38.0	25.5	38.0
18-19	34.501837898939954	38.0	34.0	38.0	26.0	38.0
20-21	34.428317875193656	37.5	34.0	38.0	25.5	38.0
22-23	34.512041897589995	38.0	34.5	38.0	25.5	38.0
24-25	34.478760475414035	37.5	34.0	38.0	25.5	38.0
26-27	34.42734809887246	38.0	34.0	38.0	25.5	38.0
28-29	34.519692355234966	38.0	34.0	38.0	26.0	38.0
30-31	34.5889571549975	38.0	35.0	38.0	26.0	38.0
32-33	34.54204600439957	38.0	34.5	38.0	26.0	38.0
34-35	34.561368340157685	38.0	34.5	38.0	26.0	38.0
36-37	34.4137732634498	37.5	34.0	38.0	24.5	38.0
38-39	34.428834276002	37.0	34.0	38.0	25.0	38.0
40-41	34.421814071312106	37.0	34.0	38.0	25.0	38.0
42-43	34.48967198383299	37.5	34.0	38.0	25.5	38.0
44-45	34.515609942545325	37.5	34.5	38.0	25.5	38.0
46-47	34.51252170306547	37.5	34.5	38.0	25.5	38.0
48-49	34.46518157384328	37.0	34.0	38.0	25.0	38.0
50-51	34.34320729629614	37.0	34.0	38.0	24.5	38.0
52-53	34.415501376391134	37.0	34.0	38.0	25.0	38.0
54-55	34.37144059560937	37.0	34.0	38.0	25.0	38.0
56-57	34.34608816222304	37.0	34.0	38.0	24.5	38.0
58-59	34.35660932042467	37.0	34.0	38.0	25.0	38.0
60-61	34.33869590498225	37.0	34.0	38.0	24.5	38.0
62-63	34.31204433727885	37.0	34.0	38.0	24.5	38.0
64-65	34.33081977611789	37.0	34.0	38.0	24.0	38.0
66-67	34.23065225081628	37.0	34.0	38.0	24.0	38.0
68-69	33.99596231493943	37.0	34.0	38.0	23.5	38.0
70-71	34.16157449386621	37.0	34.0	38.0	24.0	38.0
72-73	34.1237999780428	37.0	34.0	38.0	24.0	38.0
74-75	34.13740530957619	37.0	34.0	38.0	24.0	38.0
76	32.72945070405354	37.0	31.0	38.0	21.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	5.0
13	23.0
14	90.0
15	265.0
16	576.0
17	999.0
18	1361.0
19	1490.0
20	1448.0
21	1439.0
22	1550.0
23	2009.0
24	2364.0
25	2842.0
26	3514.0
27	4259.0
28	5197.0
29	6311.0
30	7808.0
31	9482.0
32	11719.0
33	14517.0
34	18708.0
35	25432.0
36	38601.0
37	83923.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	47.257179801002714	18.703874632521867	9.493235962640231	24.545709603835192
2	24.111444987049317	20.707265800034968	31.10115356621519	24.080135646700523
3	19.42480269016358	22.96438460881622	29.7442799461642	27.866532754856
4	13.862718707940779	17.09693290449025	38.373052823329935	30.66729556423904
5	13.310942411144499	34.35325881439254	36.776683080351155	15.55911569411181
6	28.741567825383335	38.46372792589852	18.96248165150671	13.832222597211436
7	28.569163146060106	29.91383832181936	22.635026612939296	18.88197191918124
8	25.555334176381372	34.144258802194095	19.9407968837041	20.359610137720434
9	24.766094830705924	16.373565157990182	21.767310608987	37.09302940231689
10-11	25.19608999198969	24.577018944183983	28.124326544221397	22.102564519604933
12-13	25.33596548653495	23.24088267942895	26.62920388886404	24.79394794517206
14-15	23.664575311161983	24.5235490967052	24.182805886155986	27.629069705976832
16-17	23.972992644338092	26.710526850808957	25.62486530884428	23.69161519600867
18-19	23.081489674016908	26.754237942854353	26.931725307299143	23.232547075829597
20-21	23.024766908060325	27.012235039624617	26.642825484989814	23.320172567325248
22-23	23.445409928720423	26.33278169257481	27.0376484652324	23.184159913472367
24-25	23.068071385295998	26.641199025750918	27.00898212114682	23.281747467806273
26-27	22.353039242395287	26.91017472238374	27.23587318497314	23.500912850247833
28-29	22.99345756771153	26.455172750301912	28.01067770490337	22.54069197708319
30-31	23.34009669300175	26.37283325133268	27.307030776674946	22.98003927899062
32-33	22.62384470567187	27.018740876580203	26.98519515477793	23.372219262969995
34-35	23.710319477256	26.169932461280105	27.14519808240456	22.974549979059336
36-37	23.732073369576266	25.89526415731114	26.735127046797295	23.6375354263153
38-39	23.09226496647461	26.63286342215156	27.39099673488308	22.88387487649075
40-41	23.124590843847713	25.658817645456285	27.93484404288973	23.281747467806273
42-43	22.8739128136525	25.925963575445344	27.556285655036127	23.64383795586603
44-45	22.25463845844193	25.968861437871286	27.649603753867925	24.126896349818853
46-47	22.67426494207772	25.28981470563121	28.181252617582835	23.854667734708237
48-49	21.955776573294354	25.53459682108542	28.627105756445857	23.88252084917437
50-51	21.927516844018495	25.443921718516833	28.314418967767647	24.314142469697032
52-53	21.680295039705936	25.26338474299911	28.766981250991126	24.28933896630383
54-55	20.943915619294685	25.884488864853434	28.914785734326014	24.256809781525863
56-57	20.74911255596082	25.94955495647153	28.727366619092347	24.5739658684753
58-59	21.151664491259744	25.666142129296425	28.277240364167184	24.904953015276643
60-61	20.805996791789582	25.467864019045955	28.57929952039491	25.146839668769555
62-63	20.256046907370546	25.108617643530824	29.21748535669485	25.417850092403775
64-65	21.15792405608879	25.28244653490911	28.380463665695526	25.17916574330657
66-67	21.16040678076371	25.044423029223644	27.844598688249956	25.95057150176269
68-69	21.01056800484689	25.093623821315735	27.517881031671486	26.37792714216589
70-71	21.44239644453119	24.99684870959741	27.637223448853543	25.92353139701786
72-73	21.499119671777855	24.372486591767608	27.35645363943545	26.77194009701908
74-75	21.69023018651573	24.836437862652534	27.284888850937865	26.18844309989387
76	22.16782619656573	24.635164861974605	26.99962998052315	26.19737896093651
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	1.5
11	1.5
12	0.0
13	3.5
14	7.5
15	13.5
16	38.5
17	58.0
18	54.0
19	85.0
20	131.5
21	143.0
22	177.0
23	310.5
24	519.0
25	628.0
26	770.0
27	1029.5
28	1344.5
29	1542.0
30	1866.5
31	2444.5
32	3352.5
33	4007.0
34	4361.0
35	5400.0
36	7244.5
37	8404.0
38	9170.0
39	11139.0
40	13295.5
41	14918.5
42	15588.0
43	15922.5
44	16424.5
45	16588.5
46	16585.0
47	16322.5
48	15191.5
49	13544.5
50	12766.0
51	11753.0
52	9579.5
53	7791.0
54	7163.0
55	6589.0
56	5471.5
57	4333.0
58	3738.0
59	3295.5
60	2638.0
61	2281.5
62	2140.0
63	2019.5
64	1813.0
65	1700.0
66	1647.0
67	1621.0
68	1636.0
69	1619.0
70	1494.5
71	1402.0
72	1391.0
73	1283.5
74	1144.0
75	1101.0
76	1053.0
77	951.0
78	800.5
79	704.0
80	621.0
81	453.0
82	324.0
83	280.0
84	232.0
85	146.5
86	86.0
87	63.0
88	47.0
89	26.0
90	14.5
91	8.0
92	8.0
93	5.5
94	2.5
95	2.0
96	2.0
97	1.5
98	1.5
99	3.0
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	8.132296194491996E-4
58-59	8.132296194491996E-4
60-61	6.099222145868996E-4
62-63	6.099222145868996E-4
64-65	6.099222145868996E-4
66-67	8.132296194491996E-4
68-69	8.132296194491996E-4
70-71	8.132296194491996E-4
72-73	8.132296194491996E-4
74-75	8.132296194491996E-4
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	245933.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.37678268735605
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.26461316887168	29.35211460181055
2	14.309086740368052	12.127461185022097
3	5.453758839087874	6.933382594598221
4	2.535787561542644	4.298340737471724
5	1.4746698856640734	3.124588264018731
6	1.0146832929546548	2.579940804121814
7	0.797177445498207	2.364727075979739
8	0.6674737287819735	2.2628311323290333
9	0.5357748514943966	2.043397300601565
>10	3.7793577735175727	30.202790238063056
>50	0.16262811959330603	4.47746270880218
>100	0.004988592625561535	0.2329633571813032
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.002033074048622999	0.0
2	0.0	0.0	0.0	0.002033074048622999	0.0
3	0.0	0.0	0.0	0.002033074048622999	0.0
4	0.0	0.0	0.0	0.004066148097245998	0.0
5	0.0	0.0	0.0	0.004879377716695198	0.0
6	0.0	0.0	0.0	0.004879377716695198	0.0
7	0.0	0.0	0.0	0.004879377716695198	0.0
8	0.0	0.0	0.0	0.004879377716695198	0.0
9	0.0	0.0	0.0	0.005285992526419797	0.0
10	0.0	0.0	0.0	0.006099222145868997	0.0
11	0.0	0.0	0.0	0.006099222145868997	0.0
12	0.0	0.0	0.0	0.0065058369555935965	0.0
13	0.0	0.0	0.0	0.006912451765318197	0.0
14	0.0	0.0	0.0	0.007319066575042796	0.0
15	0.0	0.0	0.0	0.007725681384767396	0.0
16	0.0	0.0	0.0	0.007725681384767396	0.0
17	0.0	0.0	0.0	0.008945525813941195	0.0
18	0.0	0.0	0.0	0.008945525813941195	0.0
19	0.0	0.0	0.0	0.009758755433390396	0.0
20	0.0	0.0	0.0	0.010571985052839595	0.0
21	0.0	0.0	0.0	0.011385214672288793	0.0
22	0.0	0.0	0.0	0.012198444291737994	0.0
23	0.0	0.0	0.0	0.012198444291737994	0.0
24	0.0	0.0	0.0	0.012198444291737994	0.0
25	0.0	0.0	0.0	0.012605059101462594	0.0
26	0.0	0.0	0.0	0.014638133150085593	0.0
27	0.0	0.0	0.0	0.022363814534852988	0.0
28	0.0	0.0	0.0	0.032529184777967984	0.0
29	0.0	0.0	0.0	0.04228794021135838	0.0
30	0.0	0.0	0.0	0.05773930298089317	0.0
31	0.0	0.0	0.0	0.06831128803373276	0.0
32	0.0	0.0	0.0	0.09148833218803495	0.0
33	0.0	0.0	0.0	0.12930350949242272	0.0
34	0.0	0.0	0.0	0.1841965088052437	0.0
35	0.0	0.0	0.0	0.26836577441823584	0.0
36	0.0	0.0	0.0	0.403361891246803	0.0
37	0.0	0.0	0.0	0.6184611255911163	0.0
38	0.0	0.0	0.0	0.9022782627788869	0.0
39	0.0	0.0	0.0	1.2145584366473796	0.0
40	0.0	0.0	0.0	1.5638405582008108	0.0
41	0.0	0.0	0.0	1.9419923312446885	0.0
42	0.0	0.0	0.0	2.3758503332208365	0.0
43	0.0	0.0	0.0	2.915834800535105	0.0
44	0.0	0.0	0.0	3.557066355470799	0.0
45	0.0	0.0	0.0	4.344272627097624	0.0
46	0.0	0.0	0.0	5.254276571261278	0.0
47	0.0	0.0	0.0	6.186237715150061	0.0
48	0.0	0.0	0.0	7.04094204519117	0.0
49	0.0	0.0	0.0	7.837093842631936	0.0
50	0.0	0.0	0.0	8.58973785543217	0.0
51	0.0	0.0	0.0	9.280576417154265	0.0
52	0.0	0.0	0.0	9.97670097140278	0.0
53	0.0	0.0	0.0	10.697222414234771	0.0
54	0.0	0.0	0.0	11.478329463715728	0.0
55	0.0	0.0	0.0	12.302131068217767	0.0
56	0.0	0.0	0.0	13.165780924072816	0.0
57	0.0	0.0	0.0	14.008286809822188	0.0
58	0.0	0.0	0.0	14.910565072601074	0.0
59	0.0	0.0	0.0	15.74819158063375	0.0
60	0.0	0.0	0.0	16.55084921503011	0.0
61	0.0	0.0	0.0	17.339681945895833	0.0
62	0.0	0.0	0.0	18.03418004090545	0.0
63	0.0	0.0	0.0	18.788450512944582	0.0
64	0.0	0.0	0.0	19.497586741104286	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGGTCTA	20	0.0069455723	52.5	1
CACTTCG	20	0.0069455723	52.5	67
GTTTGCG	35	2.0826466E-5	50.0	38
TACTCGC	30	5.870341E-4	46.666668	20
GCGGTTT	40	4.5842247E-5	43.75	42
CGCGGTA	35	0.0012538452	40.0	43
TAACGGC	35	0.0012538452	40.0	36
TATCCAC	45	9.180815E-5	38.888885	65
ACCACGT	55	6.6777593E-6	38.18182	36
AACTCTC	55	6.6777593E-6	38.18182	19
GTAACCA	55	6.6777593E-6	38.18182	33
ACGTTCT	60	1.2126682E-5	35.000004	39
AGTAACC	60	1.2126682E-5	35.000004	32
GTGCTAG	70	8.6629916E-7	35.0	27
ACTCGCA	40	0.0024157492	35.0	21
TTTGCGG	50	1.7065837E-4	35.0	39
GGTCTTT	50	1.7065837E-4	35.0	58
TTAACGG	55	2.9866648E-4	31.818184	35
TAGATGG	55	2.9866648E-4	31.818184	32
ATTAGCG	45	0.0043019424	31.111109	8
>>END_MODULE