FastQCFastQC Report
Wed 25 May 2016
SRR1781212_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781212_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences223719
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCCGT29291.3092316700861348No Hit
TCTTATACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCCG3270.14616550225953093No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAA150.002222556270.030
TATGCCG4400.058.0681843
TCTCGTA4250.056.82353638
TCGTATG4500.056.77777540
CCGTCTT4200.056.66666447
CGTCTTC4150.056.5060248
CGTATGC4550.056.15384741
TACCTCG252.3868619E-456.00000436
ATCTCGT4350.055.51724237
CTCGTAT4550.055.38461739
GCCGTCT4500.053.66666446
GTATGCC4800.053.22916442
TCTTCTG4350.053.1034550
GTCTTCT4350.053.1034549
ATGCCGT4700.052.8723444
GGATGTT200.00694475752.515
CGTAAGA200.00694475752.532
TGCCGTC4700.052.1276645
CTTGAAA4700.051.3829857
CTGCTTG4750.048.63157754
Produced by FastQC (version 0.11.5)