##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1781212_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	223719
Sequences flagged as poor quality	0
Sequence length	76
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.2427330713976	34.0	34.0	34.0	32.0	34.0
2	33.273052355857125	34.0	34.0	34.0	32.0	34.0
3	33.317424984020136	34.0	34.0	34.0	33.0	34.0
4	33.358060781605495	34.0	34.0	34.0	33.0	34.0
5	33.384383981691315	34.0	34.0	34.0	33.0	34.0
6	36.810784957915956	38.0	37.0	38.0	34.0	38.0
7	36.70875964938159	38.0	37.0	38.0	34.0	38.0
8	36.49315435881619	38.0	37.0	38.0	34.0	38.0
9	36.432404042571264	38.0	37.0	38.0	34.0	38.0
10-11	36.34450136108243	38.0	37.0	38.0	34.0	38.0
12-13	36.284318721253	38.0	37.0	38.0	34.0	38.0
14-15	36.30418292590258	38.0	37.0	38.0	34.0	38.0
16-17	36.251849418243424	38.0	37.0	38.0	34.0	38.0
18-19	36.24984914111005	38.0	37.0	38.0	34.0	38.0
20-21	36.18519213835213	38.0	37.0	38.0	33.5	38.0
22-23	36.232993174473336	38.0	37.0	38.0	34.0	38.0
24-25	36.23376646596847	38.0	37.0	38.0	34.0	38.0
26-27	36.001150997456634	38.0	36.5	38.0	32.0	38.0
28-29	35.98782177642489	38.0	37.0	38.0	32.0	38.0
30-31	36.01959824601397	38.0	37.0	38.0	32.0	38.0
32-33	35.973681269807216	38.0	37.0	38.0	32.0	38.0
34-35	35.97311582833822	38.0	37.0	38.0	32.0	38.0
36-37	35.94133488885611	38.0	37.0	38.0	32.0	38.0
38-39	35.84872764494746	38.0	36.0	38.0	31.0	38.0
40-41	35.92155114228117	38.0	37.0	38.0	32.0	38.0
42-43	35.93653869362906	38.0	36.5	38.0	32.0	38.0
44-45	35.90796266745337	38.0	36.5	38.0	32.0	38.0
46-47	35.92877001953343	38.0	37.0	38.0	32.0	38.0
48-49	35.91307399013942	38.0	37.0	38.0	32.0	38.0
50-51	35.819682280003036	38.0	36.0	38.0	31.0	38.0
52-53	35.8479007147359	38.0	36.0	38.0	31.0	38.0
54-55	35.853134959480414	38.0	36.0	38.0	32.0	38.0
56-57	35.81262208395353	38.0	36.0	38.0	31.0	38.0
58-59	35.796584107742305	38.0	36.0	38.0	31.0	38.0
60-61	35.797109320173966	38.0	36.0	38.0	31.0	38.0
62-63	35.7670492895105	38.0	36.0	38.0	31.0	38.0
64-65	35.733542971316695	38.0	36.0	38.0	31.0	38.0
66-67	35.688544558128726	38.0	36.0	38.0	31.0	38.0
68-69	35.60672987095418	38.0	36.0	38.0	31.0	38.0
70-71	35.48678029134763	38.0	36.0	38.0	31.0	38.0
72-73	35.39690638703016	38.0	36.0	38.0	31.0	38.0
74-75	35.39358078661178	38.0	36.0	38.0	31.0	38.0
76	34.45779303501267	37.0	34.0	38.0	25.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	2.0
21	4.0
22	34.0
23	68.0
24	178.0
25	343.0
26	727.0
27	1107.0
28	1894.0
29	3012.0
30	4640.0
31	6801.0
32	9872.0
33	13770.0
34	18793.0
35	25945.0
36	38782.0
37	97747.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.89739360537102	18.48211372301861	9.993786848680712	26.626705822929658
2	23.49062886925116	20.784108636280337	31.298191034288546	24.42707146017996
3	19.46012631917718	22.57608875419611	30.295147037131404	27.668637889495308
4	13.519638475051293	18.394056830220055	37.34685028987256	30.739454404856094
5	12.951962059547913	34.74671351114568	37.1264845632244	15.174839866082005
6	28.95373213718996	39.52234723023078	18.027525601312362	13.496395031266903
7	27.718700691492455	30.113222390588195	21.98561588421189	20.18246103370746
8	24.710909667931645	36.04655840585735	19.017606908666675	20.22492501754433
9	24.063222167093542	16.131843964973918	20.50116440713574	39.30376946079681
10-11	24.18122734322968	25.627014245549102	28.11026332139872	22.0814950898225
12-13	24.343931449720408	23.48303005109088	26.78158761660833	25.39145088258038
14-15	22.681354735181188	24.238888963387105	23.67501195696387	29.404744344467836
16-17	23.850008269302116	27.539904970074065	24.927922974803213	23.682163785820606
18-19	23.491746342510023	26.760802613993445	25.75418270240793	23.993268341088598
20-21	22.420312981910342	26.875678865004758	25.676853552894478	25.027154600190414
22-23	22.67085048654786	27.332054943925193	26.144404364403563	23.85269020512339
24-25	23.337535032786665	27.35574537701313	26.047407685534086	23.25931190466612
26-27	22.80070087922796	27.63734863824709	26.26308002449501	23.29887045802994
28-29	23.23673894483705	26.411927462575818	27.094927118394057	23.256406474193074
30-31	23.792123154492913	26.027740156178066	27.30121268198052	22.878924007348502
32-33	22.080824605867182	27.755130319731446	26.231120289291475	23.932924785109893
34-35	23.06889446135554	26.115797048976617	28.001868415288822	22.81344007437902
36-37	23.143988664351262	26.514064518436076	26.927753118867866	23.4141936983448
38-39	22.051993795788466	26.660900504650925	27.497440986237198	23.78966471332341
40-41	22.334491035629515	25.201256933921574	28.35387249183127	24.11037953861764
42-43	23.143541675047715	25.685793338965397	27.570523737366965	23.60014124861992
44-45	21.735748863529697	26.708951854782114	27.827095597602348	23.728203684085837
46-47	22.758460390042867	25.09934337271309	27.34233569790675	24.799860539337292
48-49	22.22766059208203	24.996088843593974	28.075621650373904	24.70062891395009
50-51	21.410787639851776	25.002123199191846	28.47590057169932	25.11118858925706
52-53	20.998440007330625	24.912948833134426	29.796753963677645	24.291857195857304
54-55	20.10647285210465	25.57717493820373	29.240475775414694	25.07587643427693
56-57	20.931838601102275	25.374018299742083	28.218211238205072	25.475931860950567
58-59	20.195870712813843	25.384969537678963	29.199352759488466	25.21980699001873
60-61	20.823220200340607	25.95108149061993	27.853691461163333	25.372006847876133
62-63	19.114156598232604	26.75342729048494	28.066458369651215	26.06595774163124
64-65	19.59623456210693	27.221201596645795	27.199299120772043	25.98326472047524
66-67	19.84185518440544	26.846177570970724	26.615531090341012	26.696436154282825
68-69	19.817270772710412	27.473750553149262	26.052548062524867	26.656430611615463
70-71	20.336001859475502	26.619777488724694	25.998685851447572	27.04553480035223
72-73	20.31566384616416	25.583656283105142	25.861460135258962	28.239219735471732
74-75	20.237664212695393	25.792623782512887	26.063946289765287	27.905765715026437
76	20.830595523849112	25.641541397914345	26.034891985034797	27.49297109320174
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.5
10	0.5
11	0.0
12	0.0
13	2.0
14	7.0
15	16.0
16	35.0
17	48.0
18	51.5
19	73.5
20	124.5
21	157.0
22	179.5
23	272.0
24	460.5
25	579.0
26	732.0
27	954.0
28	1247.0
29	1471.0
30	1690.5
31	2173.0
32	3048.5
33	3661.0
34	3959.5
35	4919.0
36	6280.0
37	6980.0
38	7795.0
39	9845.5
40	12378.5
41	14206.0
42	14736.0
43	15240.5
44	15277.0
45	14911.5
46	15014.0
47	14712.5
48	13605.0
49	12142.0
50	11485.0
51	10538.0
52	8594.0
53	7155.0
54	6713.0
55	6210.0
56	4930.5
57	3798.5
58	3443.0
59	2958.0
60	2248.5
61	1938.5
62	1853.0
63	1762.5
64	1645.0
65	1573.5
66	1498.5
67	1468.0
68	1487.0
69	1491.5
70	1429.5
71	1382.0
72	1355.5
73	1267.5
74	1172.5
75	1139.0
76	1023.0
77	860.0
78	715.0
79	617.0
80	552.0
81	409.0
82	283.0
83	235.0
84	197.0
85	119.0
86	75.5
87	72.0
88	50.0
89	21.5
90	12.5
91	6.0
92	2.0
93	1.0
94	0.5
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	223719.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.11620380924284
#Duplication Level	Percentage of deduplicated	Percentage of total
1	61.9285611060263	22.98553095624422
2	17.96208873259791	13.333690924776171
3	6.905438604942435	7.68910999959771
4	2.9758177176164557	4.41804227624833
5	1.849800086709379	3.4328778512330205
6	1.2729418565441495	2.8348061630885173
7	0.9345344188063008	2.428045896861688
8	0.7936316778264849	2.3565276082943334
9	0.5961269810684522	1.991337347297279
>10	4.578736933378294	31.989236497570612
>50	0.19027891516932416	4.593709072541894
>100	0.010838672383062768	0.6378537361600937
>500	0.0	0.0
>1k	0.0012042969314514187	1.3092316700861348
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCCGT	2929	1.3092316700861348	No Hit
TCTTATACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCCG	327	0.14616550225953093	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0013409679106378984	0.0
2	0.0	0.0	0.0	0.0013409679106378984	0.0
3	0.0	0.0	0.0	0.0013409679106378984	0.0
4	0.0	0.0	0.0	0.0035759144283677292	0.0
5	0.0	0.0	0.0	0.004469893035459661	0.0
6	0.0	0.0	0.0	0.004469893035459661	0.0
7	0.0	0.0	0.0	0.004469893035459661	0.0
8	0.0	0.0	0.0	0.004469893035459661	0.0
9	0.0	0.0	0.0	0.004916882339005627	0.0
10	0.0	0.0	0.0	0.00581086094609756	0.0
11	0.0	0.0	0.0	0.00581086094609756	0.0
12	0.0	0.0	0.0	0.006257850249643526	0.0
13	0.0	0.0	0.0	0.006704839553189492	0.0
14	0.0	0.0	0.0	0.006704839553189492	0.0
15	0.0	0.0	0.0	0.0071518288567354584	0.0
16	0.0	0.0	0.0	0.0071518288567354584	0.0
17	0.0	0.0	0.0	0.008492796767373356	0.0
18	0.0	0.0	0.0	0.008492796767373356	0.0
19	0.0	0.0	0.0	0.008939786070919322	0.0
20	0.0	0.0	0.0	0.009833764678011255	0.0
21	0.0	0.0	0.0	0.010280753981557221	0.0
22	0.0	0.0	0.0	0.010727743285103187	0.0
23	0.0	0.0	0.0	0.011174732588649153	0.0
24	0.0	0.0	0.0	0.011174732588649153	0.0
25	0.0	0.0	0.0	0.012068711195741086	0.0
26	0.0	0.0	0.0	0.014303657713470917	0.0
27	0.0	0.0	0.0	0.021902475873752342	0.0
28	0.0	0.0	0.0	0.03218322985530956	0.0
29	0.0	0.0	0.0	0.04201699453332082	0.0
30	0.0	0.0	0.0	0.061684523889343326	0.0
31	0.0	0.0	0.0	0.07330624578153845	0.0
32	0.0	0.0	0.0	0.09789065747656658	0.0
33	0.0	0.0	0.0	0.13677872688506565	0.0
34	0.0	0.0	0.0	0.19622830425667914	0.0
35	0.0	0.0	0.0	0.2851791756623264	0.0
36	0.0	0.0	0.0	0.4317916672254033	0.0
37	0.0	0.0	0.0	0.6588622334267541	0.0
38	0.0	0.0	0.0	0.9574510881954594	0.0
39	0.0	0.0	0.0	1.2913520979442963	0.0
40	0.0	0.0	0.0	1.6654821450122699	0.0
41	0.0	0.0	0.0	2.0700074647213693	0.0
42	0.0	0.0	0.0	2.5304064473737142	0.0
43	0.0	0.0	0.0	3.0913780233239017	0.0
44	0.0	0.0	0.0	3.7658848823747646	0.0
45	0.0	0.0	0.0	4.590580147417072	0.0
46	0.0	0.0	0.0	5.553842096558629	0.0
47	0.0	0.0	0.0	6.518445013610824	0.0
48	0.0	0.0	0.0	7.4186814709524	0.0
49	0.0	0.0	0.0	8.256786415101086	0.0
50	0.0	0.0	0.0	9.038123717699435	0.0
51	0.0	0.0	0.0	9.759564453622625	0.0
52	0.0	0.0	0.0	10.476535296510354	0.0
53	0.0	0.0	0.0	11.225689369253393	0.0
54	0.0	0.0	0.0	12.040997858921235	0.0
55	0.0	0.0	0.0	12.8925124821763	0.0
56	0.0	0.0	0.0	13.790513993000147	0.0
57	0.0	0.0	0.0	14.705054108055194	0.0
58	0.0	0.0	0.0	15.642837666894632	0.0
59	0.0	0.0	0.0	16.543968102843298	0.0
60	0.0	0.0	0.0	17.394588747491273	0.0
61	0.0	0.0	0.0	18.210791215766207	0.0
62	0.0	0.0	0.0	18.93089098377876	0.0
63	0.0	0.0	0.0	19.714463232894836	0.0
64	0.0	0.0	0.0	20.450207626531498	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTCGTAA	15	0.0022225562	70.0	30
TATGCCG	440	0.0	58.06818	43
TCTCGTA	425	0.0	56.823536	38
TCGTATG	450	0.0	56.777775	40
CCGTCTT	420	0.0	56.666664	47
CGTCTTC	415	0.0	56.50602	48
CGTATGC	455	0.0	56.153847	41
TACCTCG	25	2.3868619E-4	56.000004	36
ATCTCGT	435	0.0	55.517242	37
CTCGTAT	455	0.0	55.384617	39
GCCGTCT	450	0.0	53.666664	46
GTATGCC	480	0.0	53.229164	42
TCTTCTG	435	0.0	53.10345	50
GTCTTCT	435	0.0	53.10345	49
ATGCCGT	470	0.0	52.87234	44
GGATGTT	20	0.006944757	52.5	15
CGTAAGA	20	0.006944757	52.5	32
TGCCGTC	470	0.0	52.12766	45
CTTGAAA	470	0.0	51.38298	57
CTGCTTG	475	0.0	48.631577	54
>>END_MODULE