FastQCFastQC Report
Wed 25 May 2016
SRR1781211_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781211_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences261218
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCCAA200.006946054352.540
GGTGACA659.973519E-943.07692319
GATCCTA602.5895315E-740.8333321
CGAAGCA350.00125398840.00000448
GTGCGTC459.1823815E-538.8888954
CACTAAC501.706874E-435.027
GTACAAG450.004302426731.1111131
CCAGAGT450.004302426731.1111133
GCGTCAT450.004302426731.11111356
CGTCATA450.004302426731.11111357
CTTACCG450.004302426731.1111139
GGTCGAT802.458979E-630.62510
GTTCTAG604.9738795E-429.1666661
AGTGCGT604.9738795E-429.16666653
GCTTAAC604.9738795E-429.16666613
CGTATTG755.5717923E-528.057
TGGTTCG500.007200332427.99999835
GTATACC500.007200332427.9999981
CCGAAAT500.007200332427.99999847
TATAAGA1056.74303E-726.6666662