##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1781211_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	261218
Sequences flagged as poor quality	0
Sequence length	76
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.535667526740117	34.0	33.0	34.0	23.0	34.0
2	31.583535590962338	34.0	33.0	34.0	24.0	34.0
3	31.52373113644542	34.0	33.0	34.0	23.0	34.0
4	31.66532168533562	34.0	34.0	34.0	23.0	34.0
5	31.66814691177484	34.0	34.0	34.0	23.0	34.0
6	34.48702616205621	37.0	34.0	38.0	26.0	38.0
7	34.45426425437757	37.0	34.0	38.0	25.0	38.0
8	34.26891332144033	37.0	34.0	38.0	24.0	38.0
9	34.50473550827278	37.0	34.0	38.0	26.0	38.0
10-11	34.36804699523004	37.0	34.0	38.0	25.0	38.0
12-13	34.330325628402335	37.0	34.0	38.0	25.0	38.0
14-15	34.189334195958935	37.0	34.0	38.0	24.0	38.0
16-17	34.14329793505807	37.0	34.0	38.0	24.0	38.0
18-19	34.19842047638371	37.0	34.0	38.0	24.0	38.0
20-21	34.15427535621588	37.0	34.0	38.0	24.0	38.0
22-23	34.188486245205155	37.0	34.0	38.0	24.5	38.0
24-25	34.15306563866196	37.0	34.0	38.0	24.0	38.0
26-27	34.085344424963054	37.0	34.0	38.0	24.0	38.0
28-29	34.15141567579569	37.0	34.0	38.0	24.0	38.0
30-31	34.21180584798904	37.0	34.0	38.0	24.0	38.0
32-33	34.171117610578136	37.0	34.0	38.0	24.0	38.0
34-35	34.189942882956	37.0	34.0	38.0	24.0	38.0
36-37	34.04617790504483	37.0	34.0	38.0	23.5	38.0
38-39	34.06502231852322	37.0	34.0	38.0	24.0	38.0
40-41	34.07601505256146	37.0	34.0	38.0	24.0	38.0
42-43	34.16603373427559	37.0	34.0	38.0	24.0	38.0
44-45	34.18028811184528	37.0	34.0	38.0	24.0	38.0
46-47	34.177991179780875	37.0	34.0	38.0	24.0	38.0
48-49	34.14187192306809	37.0	34.0	38.0	24.0	38.0
50-51	34.02214242510087	37.0	34.0	38.0	24.0	38.0
52-53	34.115606504911604	37.0	34.0	38.0	24.0	38.0
54-55	34.08144538278373	37.0	34.0	38.0	24.0	38.0
56-57	34.0617511044415	37.0	34.0	38.0	24.0	38.0
58-59	34.04641908291159	37.0	34.0	38.0	24.0	38.0
60-61	34.02713059590074	37.0	34.0	38.0	24.0	38.0
62-63	33.99253114257057	37.0	34.0	38.0	24.0	38.0
64-65	33.99354753500907	37.0	34.0	38.0	24.0	38.0
66-67	33.88332924989855	37.0	34.0	38.0	23.0	38.0
68-69	33.63893567824576	37.0	34.0	38.0	22.5	38.0
70-71	33.80165034568827	37.0	34.0	38.0	23.0	38.0
72-73	33.77529687846932	37.0	34.0	38.0	23.0	38.0
74-75	33.7682452970316	37.0	34.0	38.0	23.0	38.0
76	32.36688513042746	37.0	31.0	38.0	21.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	5.0
13	31.0
14	122.0
15	292.0
16	689.0
17	1129.0
18	1451.0
19	1657.0
20	1748.0
21	1817.0
22	1948.0
23	2463.0
24	2919.0
25	3505.0
26	4485.0
27	5182.0
28	6098.0
29	7608.0
30	8964.0
31	10806.0
32	13230.0
33	16406.0
34	20631.0
35	26851.0
36	40529.0
37	80651.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.928619007878474	17.9003743999265	9.7386091310706	25.432397461124424
2	24.331784180263227	20.003598526900905	30.37960630584416	25.285010986991708
3	20.204197260525692	21.194940624306135	28.855591881110797	29.745270234057376
4	14.707638830402193	16.157002963042363	35.45352923611696	33.68182897043848
5	14.594706337235566	32.87560581583199	35.12545077291764	17.40423707401481
6	30.862344861380148	35.515163579845186	18.376222159269272	15.246269399505394
7	30.340941282759992	28.15923864358505	21.550582272278326	19.94923780137663
8	26.947224157600164	33.588420399819306	19.315284551600577	20.14907089097995
9	25.060294466690657	16.73353291120826	21.241644909615722	36.964527712485356
10-11	26.25412490716566	23.907234570358856	26.554448774586742	23.284191747888734
12-13	26.01065010833863	22.271053296480332	26.32379851311931	25.394498082061727
14-15	23.901109418187108	23.425835891860437	23.47847391833641	29.194580771616046
16-17	24.17597562189436	26.486306456676033	24.64895221615662	24.688765705272992
18-19	23.608250579975344	25.97275072927593	26.066733532911208	24.352265157837515
20-21	23.806935203546466	26.01868937056405	26.027685687816305	24.146689738073178
22-23	24.026100804691865	25.651754473275197	26.293746985276666	24.028397736756272
24-25	23.665291059574763	25.73922930272799	26.228093010435728	24.36738662726152
26-27	23.07459669701169	26.036682005068563	26.410699109556003	24.47802218836374
28-29	23.5135021323186	25.744397399872902	26.90645361345696	23.835646854351538
30-31	23.67754136391826	25.95590656080362	26.20722921085071	24.159322864427413
32-33	23.355779463896056	26.083003468367416	26.266949444525263	24.29426762321126
34-35	23.966763393028046	25.454983959757747	26.5249714797602	24.053281167454006
36-37	24.089075025457664	25.448093163564533	26.045104089304722	24.417727721673085
38-39	23.305246958479124	25.88451025580167	26.854198408991724	23.956044376727483
40-41	23.60518800388947	25.217060080086362	27.07393824315323	24.103813672870935
42-43	23.197099740446678	25.213997504000492	26.97421310935694	24.6146896461959
44-45	22.66918818764404	25.253045349095395	27.28334188302491	24.794424580235667
46-47	22.851411464753575	24.723602508249815	27.66290990666799	24.762076120328615
48-49	22.291534274054623	24.682066320085138	28.344524496780465	24.681874909079774
50-51	22.115818971127563	24.926306762933642	27.941030097466484	25.016844168472314
52-53	21.765536831305653	24.910036827477434	28.396014057224235	24.928412283992678
54-55	21.195746839319725	25.38784729200762	28.402384985692	25.01402088298066
56-57	20.995107534702814	25.481015856487684	28.24441654097344	25.279460067836062
58-59	21.36204243199167	25.262615899363748	27.936819055348405	25.438522613296172
60-61	21.093684202466907	25.07618158013613	28.074634979212764	25.7554992381842
62-63	20.867627805128283	24.955784057760187	28.28805825019715	25.88852988691438
64-65	21.541203133015337	24.843808619620393	27.604146728020275	26.010841519343998
66-67	21.591927049437633	24.83404665834667	27.116048664334002	26.45797762788169
68-69	21.49373320368428	24.681683498074406	27.071258489078087	26.753324809163225
70-71	21.926130664808703	24.75403685810319	26.913344409650175	26.406488067437927
72-73	22.048250886232953	24.34460871762283	26.62469661355649	26.982443782587723
74-75	22.217649625982897	24.64340129700097	26.479032838472083	26.659916238544053
76	22.67301640775138	24.4117939805067	26.006247655215187	26.908941956526732
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.5
6	0.5
7	0.5
8	1.0
9	0.5
10	0.5
11	0.5
12	0.0
13	2.5
14	8.0
15	23.5
16	47.5
17	59.0
18	77.0
19	119.0
20	206.5
21	270.0
22	329.5
23	486.5
24	707.0
25	830.0
26	994.0
27	1252.0
28	1735.5
29	2125.0
30	2398.5
31	2901.0
32	3745.5
33	4361.0
34	4762.0
35	5841.0
36	7325.5
37	8132.0
38	8804.5
39	10470.5
40	12376.0
41	13811.0
42	14334.0
43	14586.5
44	14933.0
45	15180.5
46	15334.0
47	15147.0
48	14083.5
49	12710.0
50	12213.0
51	11407.0
52	9749.0
53	8309.0
54	7721.0
55	7228.0
56	6281.5
57	5441.0
58	5054.0
59	4722.5
60	4257.5
61	4037.0
62	3950.0
63	3889.0
64	3606.0
65	3340.5
66	3232.5
67	3168.0
68	3057.0
69	2791.0
70	2518.0
71	2400.0
72	2270.0
73	2034.0
74	1781.5
75	1635.0
76	1505.5
77	1264.5
78	1025.0
79	897.0
80	793.5
81	579.5
82	381.5
83	294.0
84	244.5
85	160.0
86	101.0
87	77.0
88	64.0
89	33.5
90	15.0
91	10.5
92	7.0
93	7.0
94	5.0
95	2.5
96	2.0
97	1.0
98	0.0
99	2.5
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	1.9141100536716459E-4
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	261218.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.235527748578896
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.78566106087973	40.7558992897546
2	14.208232726515776	15.69598466047458
3	4.954710392648912	8.210281301379938
4	2.0154480811202022	4.452973536421402
5	1.0675437346157817	2.948317078809578
6	0.7075852366103147	2.345030638276428
7	0.5008592801245833	1.9365658665818273
8	0.3944249771132377	1.742901741445667
9	0.3506530259093353	1.743165444846442
>10	1.9837025088381743	19.052520445484163
>50	0.029793243373979608	1.034460428730947
>100	0.00138573224995254	0.08189956779445898
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	7.656440214686583E-4	0.0
2	0.0	0.0	0.0	0.0015312880429373167	0.0
3	0.0	0.0	0.0	0.0015312880429373167	0.0
4	0.0	0.0	0.0	0.002296932064405975	0.0
5	0.0	0.0	0.0	0.002296932064405975	0.0
6	0.0	0.0	0.0	0.0026797540751403045	0.0
7	0.0	0.0	0.0	0.0026797540751403045	0.0
8	0.0	0.0	0.0	0.0026797540751403045	0.0
9	0.0	0.0	0.0	0.0034453980966089627	0.0
10	0.0	0.0	0.0	0.00459386412881195	3.8282201073432917E-4
11	0.0	0.0	0.0	0.004976686139546279	3.8282201073432917E-4
12	0.0	0.0	0.0	0.004976686139546279	3.8282201073432917E-4
13	0.0	0.0	0.0	0.004976686139546279	3.8282201073432917E-4
14	0.0	0.0	0.0	0.004976686139546279	3.8282201073432917E-4
15	0.0	0.0	0.0	0.005359508150280609	3.8282201073432917E-4
16	0.0	0.0	0.0	0.005742330161014938	3.8282201073432917E-4
17	0.0	0.0	0.0	0.005742330161014938	3.8282201073432917E-4
18	0.0	0.0	0.0	0.006125152171749267	3.8282201073432917E-4
19	0.0	0.0	0.0	0.006125152171749267	3.8282201073432917E-4
20	0.0	0.0	0.0	0.006125152171749267	3.8282201073432917E-4
21	0.0	0.0	0.0	0.007273618203952254	3.8282201073432917E-4
22	0.0	0.0	0.0	0.0091877282576239	3.8282201073432917E-4
23	0.0	0.0	0.0	0.009953372279092558	3.8282201073432917E-4
24	0.0	0.0	0.0	0.011484660322029876	3.8282201073432917E-4
25	0.0	0.0	0.0	0.012633126354232863	3.8282201073432917E-4
26	0.0	0.0	0.0	0.014930058418638838	3.8282201073432917E-4
27	0.0	0.0	0.0	0.029094472815809017	3.8282201073432917E-4
28	0.0	0.0	0.0	0.04364170922371353	3.8282201073432917E-4
29	0.0	0.0	0.0	0.05895458965308669	3.8282201073432917E-4
30	0.0	0.0	0.0	0.08460366437228675	3.8282201073432917E-4
31	0.0	0.0	0.0	0.10642451898414351	3.8282201073432917E-4
32	0.0	0.0	0.0	0.13283923772481224	3.8282201073432917E-4
33	0.0	0.0	0.0	0.1730355488519168	3.8282201073432917E-4
34	0.0	0.0	0.0	0.23505271459087812	3.8282201073432917E-4
35	0.0	0.0	0.0	0.32616435314564846	3.8282201073432917E-4
36	0.0	0.0	0.0	0.48426984357892644	3.8282201073432917E-4
37	0.0	0.0	0.0	0.7403777687601927	3.8282201073432917E-4
38	0.0	0.0	0.0	1.0546746395730768	3.8282201073432917E-4
39	0.0	0.0	0.0	1.4183555497706897	3.8282201073432917E-4
40	0.0	0.0	0.0	1.829506389299359	3.8282201073432917E-4
41	0.0	0.0	0.0	2.3019087505455214	3.8282201073432917E-4
42	0.0	0.0	0.0	2.8543209120351585	3.8282201073432917E-4
43	0.0	0.0	0.0	3.4921023819185506	3.8282201073432917E-4
44	0.0	0.0	0.0	4.1999402797663254	3.8282201073432917E-4
45	0.0	0.0	0.0	5.006163434372823	3.8282201073432917E-4
46	0.0	0.0	0.0	5.882060194932968	3.8282201073432917E-4
47	0.0	0.0	0.0	6.736901744902725	3.8282201073432917E-4
48	0.0	0.0	0.0	7.557672135917127	3.8282201073432917E-4
49	0.0	0.0	0.0	8.350496520147923	3.8282201073432917E-4
50	0.0	0.0	0.0	9.07364729842507	3.8282201073432917E-4
51	0.0	0.0	0.0	9.808282737024248	3.8282201073432917E-4
52	0.0	0.0	0.0	10.52377707508671	3.8282201073432917E-4
53	0.0	0.0	0.0	11.261092267761027	3.8282201073432917E-4
54	0.0	0.0	0.0	12.044346101723464	3.8282201073432917E-4
55	0.0	0.0	0.0	12.919094396251406	3.8282201073432917E-4
56	0.0	0.0	0.0	13.690863569891814	3.8282201073432917E-4
57	0.0	0.0	0.0	14.43238980468421	3.8282201073432917E-4
58	0.0	0.0	0.0	15.189994563927447	3.8282201073432917E-4
59	0.0	0.0	0.0	15.919653316387079	3.8282201073432917E-4
60	0.0	0.0	0.0	16.649694890857443	3.8282201073432917E-4
61	0.0	0.0	0.0	17.34681377240466	3.8282201073432917E-4
62	0.0	0.0	0.0	17.99952530070669	3.8282201073432917E-4
63	0.0	0.0	0.0	18.706597554532994	3.8282201073432917E-4
64	0.0	0.0	0.0	19.3880207336401	3.8282201073432917E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTCCAA	20	0.0069460543	52.5	40
GGTGACA	65	9.973519E-9	43.076923	19
GATCCTA	60	2.5895315E-7	40.833332	1
CGAAGCA	35	0.001253988	40.000004	48
GTGCGTC	45	9.1823815E-5	38.88889	54
CACTAAC	50	1.706874E-4	35.0	27
GTACAAG	45	0.0043024267	31.111113	1
CCAGAGT	45	0.0043024267	31.111113	3
GCGTCAT	45	0.0043024267	31.111113	56
CGTCATA	45	0.0043024267	31.111113	57
CTTACCG	45	0.0043024267	31.111113	9
GGTCGAT	80	2.458979E-6	30.625	10
GTTCTAG	60	4.9738795E-4	29.166666	1
AGTGCGT	60	4.9738795E-4	29.166666	53
GCTTAAC	60	4.9738795E-4	29.166666	13
CGTATTG	75	5.5717923E-5	28.0	57
TGGTTCG	50	0.0072003324	27.999998	35
GTATACC	50	0.0072003324	27.999998	1
CCGAAAT	50	0.0072003324	27.999998	47
TATAAGA	105	6.74303E-7	26.666666	2
>>END_MODULE