FastQCFastQC Report
Wed 25 May 2016
SRR1781211_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781211_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences243568
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCGT30901.2686395585627013No Hit
TCTTATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCG3260.13384352624318466No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGTCG252.834271E-670.057
TCGTATG5450.055.2293640
CGTATGC5600.054.37541
CTCGTAT5450.053.94495439
TATGCCG5450.053.94495443
GGGTCTA200.006945493652.51
GACGTAA200.006945493652.553
TCTCGTA4800.052.538
AGTCGAC200.006945493652.518
CCGTCTT5350.051.68224347
GTATGCC5900.051.61017242
GCCGTCT5550.050.4504546
ATCTCGT5000.050.39999837
CGTCTTC5350.050.37383348
CTTGAAA5500.050.27272457
GTCTTCT5400.049.2592649
CTGCTTG5550.049.1891954
TCTTCTG5550.049.1891950
CATCTCG5200.048.4615436
ATGCCGT5800.048.2758644
Produced by FastQC (version 0.11.5)