##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1781211_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	243568
Sequences flagged as poor quality	0
Sequence length	76
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.19063259541483	34.0	34.0	34.0	32.0	34.0
2	33.303779642646	34.0	34.0	34.0	33.0	34.0
3	33.33142695263746	34.0	34.0	34.0	33.0	34.0
4	33.42327399329961	34.0	34.0	34.0	34.0	34.0
5	33.4352993825133	34.0	34.0	34.0	34.0	34.0
6	36.8617634500427	38.0	37.0	38.0	34.0	38.0
7	36.76462014714576	38.0	37.0	38.0	34.0	38.0
8	36.53145733429679	38.0	37.0	38.0	34.0	38.0
9	36.51259196610392	38.0	37.0	38.0	34.0	38.0
10-11	36.44219273467779	38.0	37.0	38.0	34.0	38.0
12-13	36.38216432372069	38.0	37.0	38.0	34.0	38.0
14-15	36.388727172699205	38.0	37.0	38.0	34.0	38.0
16-17	36.33050113315378	38.0	37.0	38.0	34.0	38.0
18-19	36.333687101753924	38.0	37.0	38.0	34.0	38.0
20-21	36.26827210470998	38.0	37.0	38.0	34.0	38.0
22-23	36.330813161006375	38.0	37.0	38.0	34.0	38.0
24-25	36.320830322538264	38.0	37.0	38.0	34.0	38.0
26-27	36.11523886553242	38.0	37.0	38.0	33.0	38.0
28-29	36.09770782697234	38.0	37.0	38.0	32.5	38.0
30-31	36.1176262891677	38.0	37.0	38.0	33.5	38.0
32-33	36.02258506864612	38.0	37.0	38.0	32.0	38.0
34-35	36.05569286605794	38.0	37.0	38.0	32.5	38.0
36-37	36.04801739144715	38.0	37.0	38.0	32.5	38.0
38-39	35.95063801484596	38.0	37.0	38.0	32.0	38.0
40-41	36.00284314852526	38.0	37.0	38.0	32.0	38.0
42-43	36.02293609998029	38.0	37.0	38.0	32.0	38.0
44-45	35.98419127307364	38.0	37.0	38.0	32.0	38.0
46-47	36.01257349077055	38.0	37.0	38.0	32.0	38.0
48-49	35.985275159298425	38.0	37.0	38.0	32.0	38.0
50-51	35.906077563555144	38.0	36.5	38.0	32.0	38.0
52-53	35.92267662418708	38.0	37.0	38.0	32.0	38.0
54-55	35.94180885830651	38.0	37.0	38.0	32.0	38.0
56-57	35.88116049727386	38.0	37.0	38.0	32.0	38.0
58-59	35.883367273205025	38.0	37.0	38.0	32.0	38.0
60-61	35.888170859883076	38.0	37.0	38.0	31.5	38.0
62-63	35.84790900282468	38.0	36.0	38.0	31.0	38.0
64-65	35.819892186165674	38.0	36.0	38.0	31.0	38.0
66-67	35.78747824016291	38.0	36.0	38.0	31.0	38.0
68-69	35.70737124745451	38.0	36.0	38.0	31.0	38.0
70-71	35.59482567496551	38.0	36.0	38.0	31.0	38.0
72-73	35.48028271365696	38.0	36.0	38.0	31.0	38.0
74-75	35.491928332129014	38.0	36.0	38.0	31.0	38.0
76	34.53826446823885	37.0	34.0	38.0	25.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	7.0
21	8.0
22	28.0
23	70.0
24	146.0
25	315.0
26	608.0
27	1122.0
28	1876.0
29	3044.0
30	4786.0
31	7081.0
32	10101.0
33	14304.0
34	19880.0
35	27888.0
36	42604.0
37	109699.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.84155554095776	18.228174472837154	10.348239506010643	27.58203048019444
2	23.45833607041976	20.36720751494449	31.28571897786244	24.888737436773305
3	20.43453984103002	21.979488274321753	28.695477238389277	28.89049464625895
4	14.700617486697759	17.43660907836826	34.912221638310456	32.95055179662353
5	14.271168626420547	33.580765946265515	35.63563358076595	16.512431846547987
6	31.011462917953097	36.9605202653879	17.510510411876766	14.517506404782237
7	29.37865401037903	28.561633712146094	21.04545753136701	21.014254746107863
8	26.214034684359195	35.06084543125534	18.72988241476713	19.995237469618342
9	24.687972147408527	16.63806411351245	20.38855678906917	38.28540695000985
10-11	25.710684490573477	24.890174407147082	26.55233856664258	22.846802535636865
12-13	25.45736714182487	22.398467779018592	26.457498521973328	25.68666655718321
14-15	23.340709781252052	23.230678906917166	22.9687397359259	30.459871575904877
16-17	24.5235416803521	27.115630953162977	23.936436641923407	24.42439072456152
18-19	24.259549694541153	26.086349602575048	25.32167608224397	24.332424620639824
20-21	23.171763121592328	26.100719306312815	25.08765519280037	25.639862379294488
22-23	23.69174111541746	26.440665440451948	25.387571437955724	24.480022006174867
24-25	24.241690205609935	26.344388425408923	25.380386586086846	24.033534782894307
26-27	23.65889607830257	26.821667870984694	25.733265453589965	23.786170597122773
28-29	23.254080995861525	25.60968600144518	27.02879688629048	24.107436116402813
30-31	23.228420810615518	26.54474315180976	25.709247520199696	24.517588517375025
32-33	22.857477172699205	26.81653583393549	25.546254023517044	24.779732969848254
34-35	23.394698811009658	25.35924259344413	26.667090915062737	24.57896768048348
36-37	23.506577218682256	26.120015765617815	25.48261676410694	24.890790251592986
38-39	22.914134861722392	25.837548446429743	26.784101359784536	24.464215332063326
40-41	23.06501675096893	25.00636372594101	27.24311075346515	24.68550876962491
42-43	23.832358930565594	24.93307823687841	26.996362412139526	24.238200420416476
44-45	22.40606319385141	25.75913092031794	27.480826709584182	24.35397917624647
46-47	23.25284930696972	24.485769887669974	27.210470997832225	25.050909807528082
48-49	22.707416409380542	24.52785259147343	27.518393220784336	25.24633777836169
50-51	21.717959666294423	24.59805885830651	28.42019477107009	25.26378670432898
52-53	21.24375944294817	24.577325428627734	29.558891151547	24.620023976877096
54-55	20.773254286277343	24.941084214675165	28.699993430992578	25.585668068054918
56-57	21.49235531761151	24.941700059121068	27.633350850686462	25.932593772580965
58-59	20.705716678709845	24.78897063653682	28.876330224003155	25.628982460750183
60-61	21.41311666557183	25.552823030940026	27.32091243513105	25.71314786835709
62-63	20.00262760296919	26.291220521579188	27.256659331275046	26.44949254417657
64-65	20.337646981541088	26.80483478946331	26.459140773829077	26.39837745516652
66-67	20.482165144846615	26.51600374433423	25.809835446364055	27.1919956644551
68-69	20.277088944360507	27.092228864218615	25.52675228273008	27.103929908690798
70-71	20.77571766406096	26.5991427445313	25.30381659331275	27.32132299809499
72-73	20.60307593772581	25.75009853511134	25.49370196413322	28.153123563029624
74-75	20.637357945214475	25.908781120672664	25.27671943769296	28.17714149641989
76	20.939942849635422	26.13890166195888	24.75366222163831	28.16749326676739
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.5
10	0.5
11	1.5
12	3.0
13	3.0
14	3.5
15	18.5
16	39.5
17	46.0
18	73.0
19	122.0
20	176.5
21	209.0
22	287.5
23	485.5
24	764.0
25	923.0
26	1023.0
27	1230.0
28	1657.5
29	1978.0
30	2138.0
31	2693.5
32	3628.0
33	4167.0
34	4591.0
35	5521.0
36	6691.0
37	7355.0
38	7893.5
39	9505.5
40	11477.0
41	13134.5
42	13894.0
43	14249.0
44	14772.0
45	14642.0
46	14344.0
47	14069.5
48	13155.0
49	11764.5
50	11014.0
51	10407.0
52	8836.0
53	7352.5
54	6833.0
55	6397.0
56	5592.0
57	4889.0
58	4555.0
59	4259.5
60	3825.5
61	3683.0
62	3679.0
63	3478.5
64	3240.0
65	3155.0
66	3079.0
67	3050.0
68	2911.0
69	2683.0
70	2466.5
71	2339.0
72	2162.0
73	1882.5
74	1662.5
75	1545.0
76	1393.5
77	1129.0
78	921.5
79	827.0
80	734.5
81	553.0
82	394.0
83	324.0
84	265.0
85	164.5
86	108.0
87	93.0
88	70.0
89	33.5
90	15.5
91	8.0
92	5.0
93	3.5
94	1.5
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	243568.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.81509240122001
#Duplication Level	Percentage of deduplicated	Percentage of total
1	59.35832966649879	26.00790699119723
2	22.19849256124947	19.45258005477881
3	7.8179876230648375	10.276375502885406
4	3.129617988516284	5.48498005389445
5	1.5751565962620353	3.4507815905806134
6	1.0740413290139117	2.823553204408421
7	0.7576538046225211	2.323767002037015
8	0.5335276171816783	1.8701249476334378
9	0.46212559054829344	1.8223267905757659
>10	3.0256326063400825	23.05586018691404
>50	0.05950113238422091	1.5677046315196408
>100	0.0069417987781591065	0.5214095226783727
>500	0.0	0.0
>1k	9.916855397370152E-4	1.3426295208968098
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCGT	3090	1.2686395585627013	No Hit
TCTTATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCG	326	0.13384352624318466	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	8.211259278722985E-4	0.0
2	0.0	0.0	0.0	0.001642251855744597	0.0
3	0.0	0.0	0.0	0.001642251855744597	0.0
4	0.0	0.0	0.0	0.0024633777836168954	0.0
5	0.0	0.0	0.0	0.0024633777836168954	0.0
6	0.0	0.0	0.0	0.002873940747553045	0.0
7	0.0	0.0	0.0	0.002873940747553045	0.0
8	0.0	0.0	0.0	0.002873940747553045	0.0
9	0.0	0.0	0.0	0.003284503711489194	0.0
10	0.0	0.0	0.0	0.0045161926032976415	0.0
11	0.0	0.0	0.0	0.004926755567233791	0.0
12	0.0	0.0	0.0	0.004926755567233791	0.0
13	0.0	0.0	0.0	0.004926755567233791	0.0
14	0.0	0.0	0.0	0.004926755567233791	0.0
15	0.0	0.0	0.0	0.004926755567233791	0.0
16	0.0	0.0	0.0	0.00574788149510609	0.0
17	0.0	0.0	0.0	0.00574788149510609	0.0
18	0.0	0.0	0.0	0.006158444459042239	0.0
19	0.0	0.0	0.0	0.006158444459042239	0.0
20	0.0	0.0	0.0	0.006158444459042239	0.0
21	0.0	0.0	0.0	0.006979570386914537	0.0
22	0.0	0.0	0.0	0.007390133350850686	0.0
23	0.0	0.0	0.0	0.008211259278722984	0.0
24	0.0	0.0	0.0	0.009442948170531432	0.0
25	0.0	0.0	0.0	0.010264074098403731	0.0
26	0.0	0.0	0.0	0.012727451882020627	0.0
27	0.0	0.0	0.0	0.027507718583722	0.0
28	0.0	0.0	0.0	0.041466859357551074	0.0
29	0.0	0.0	0.0	0.055836563095316295	0.0
30	0.0	0.0	0.0	0.08211259278722985	0.0
31	0.0	0.0	0.0	0.10715693358733495	0.0
32	0.0	0.0	0.0	0.1342540892071208	0.0
33	0.0	0.0	0.0	0.17531038560073572	0.0
34	0.0	0.0	0.0	0.24058989686658347	0.0
35	0.0	0.0	0.0	0.3378933193194508	0.0
36	0.0	0.0	0.0	0.500886816002102	0.0
37	0.0	0.0	0.0	0.7710372462720883	0.0
38	0.0	0.0	0.0	1.1064671878079222	0.0
39	0.0	0.0	0.0	1.4866484924127965	0.0
40	0.0	0.0	0.0	1.9103494711949025	0.0
41	0.0	0.0	0.0	2.4026144649543455	0.0
42	0.0	0.0	0.0	2.982329370032188	0.0
43	0.0	0.0	0.0	3.639230112330027	0.0
44	0.0	0.0	0.0	4.3692110622085005	0.0
45	0.0	0.0	0.0	5.2038855678906915	0.0
46	0.0	0.0	0.0	6.118209288576496	0.0
47	0.0	0.0	0.0	6.998045720291664	0.0
48	0.0	0.0	0.0	7.838057544505025	0.0
49	0.0	0.0	0.0	8.655898968665834	0.0
50	0.0	0.0	0.0	9.399017933390265	0.0
51	0.0	0.0	0.0	10.150348157393418	0.0
52	0.0	0.0	0.0	10.884024173947317	0.0
53	0.0	0.0	0.0	11.659577612822703	0.0
54	0.0	0.0	0.0	12.456069762858831	0.0
55	0.0	0.0	0.0	13.347401957564212	0.0
56	0.0	0.0	0.0	14.141020166852789	0.0
57	0.0	0.0	0.0	14.943260198384024	0.0
58	0.0	0.0	0.0	15.715939696511857	0.0
59	0.0	0.0	0.0	16.496830453918413	0.0
60	0.0	0.0	0.0	17.250213492741246	0.0
61	0.0	0.0	0.0	17.949812783288444	0.0
62	0.0	0.0	0.0	18.616977599684688	0.0
63	0.0	0.0	0.0	19.34080010510412	0.0
64	0.0	0.0	0.0	20.050663469749722	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTAGTCG	25	2.834271E-6	70.0	57
TCGTATG	545	0.0	55.22936	40
CGTATGC	560	0.0	54.375	41
CTCGTAT	545	0.0	53.944954	39
TATGCCG	545	0.0	53.944954	43
GGGTCTA	20	0.0069454936	52.5	1
GACGTAA	20	0.0069454936	52.5	53
TCTCGTA	480	0.0	52.5	38
AGTCGAC	20	0.0069454936	52.5	18
CCGTCTT	535	0.0	51.682243	47
GTATGCC	590	0.0	51.610172	42
GCCGTCT	555	0.0	50.45045	46
ATCTCGT	500	0.0	50.399998	37
CGTCTTC	535	0.0	50.373833	48
CTTGAAA	550	0.0	50.272724	57
GTCTTCT	540	0.0	49.25926	49
CTGCTTG	555	0.0	49.18919	54
TCTTCTG	555	0.0	49.18919	50
CATCTCG	520	0.0	48.46154	36
ATGCCGT	580	0.0	48.27586	44
>>END_MODULE