FastQCFastQC Report
Wed 25 May 2016
SRR1781210_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781210_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences820019
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTATCG400.002419030335.035
TTACGTC604.983062E-429.16666633
CGCGGTA500.00720918228.00000243
ATCGATA500.00720918228.00000223
AATCGAT755.586145E-528.022
TCGTTCG657.9573813E-426.92307724
CGAAACG657.9573813E-426.92307715
CGTGATA700.001226254725.00000224
CGATTGA800.002666501621.87554
GGTCCTA2001.0677468E-921.0000021
CGAAAGG850.003789852420.58823656
GCGTTGG850.003789852420.58823650
CGTCAGA1257.8228695E-519.62
TCCCGCG900.00527560219.4444432
TCGAGTG900.00527560219.44444340
GCTATAA2757.2759576E-1219.0909081
GGTATTA2951.8189894E-1218.9830511
GTATAAG4100.018.7804871
GCGGATG1501.4956189E-518.66666613
CGAATCT7300.018.21917769
Produced by FastQC (version 0.11.5)