##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1781210_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	820019
Sequences flagged as poor quality	0
Sequence length	76
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.081049341539646	34.0	32.0	34.0	21.0	34.0
2	31.264093880751542	34.0	32.0	34.0	23.0	34.0
3	31.339122630085402	34.0	33.0	34.0	23.0	34.0
4	31.430107107274342	34.0	33.0	34.0	23.0	34.0
5	31.520107460924685	34.0	33.0	34.0	23.0	34.0
6	34.38675201428259	37.0	34.0	38.0	26.0	38.0
7	34.313253717291914	37.0	34.0	38.0	24.0	38.0
8	34.10080376186406	37.0	34.0	38.0	24.0	38.0
9	34.38828368610971	37.0	34.0	38.0	26.0	38.0
10-11	34.25844706037299	37.0	34.0	38.0	24.0	38.0
12-13	34.220023560429695	37.0	34.0	38.0	24.0	38.0
14-15	34.102065927740696	37.0	34.0	38.0	24.0	38.0
16-17	34.06136443179975	37.0	34.0	38.0	23.5	38.0
18-19	34.1255836755002	37.0	34.0	38.0	24.0	38.0
20-21	34.051754288620145	37.0	34.0	38.0	23.5	38.0
22-23	34.12761716496813	37.0	34.0	38.0	24.0	38.0
24-25	34.12764521309872	37.0	34.0	38.0	24.0	38.0
26-27	34.05402191900431	37.0	34.0	38.0	24.0	38.0
28-29	34.13930957697322	37.0	34.0	38.0	24.0	38.0
30-31	34.239255431886335	37.0	34.0	38.0	24.0	38.0
32-33	34.19210835358693	37.0	34.0	38.0	24.0	38.0
34-35	34.20775250329565	37.0	34.0	38.0	24.0	38.0
36-37	34.06283756839781	37.0	34.0	38.0	24.0	38.0
38-39	34.07383182584794	37.0	34.0	38.0	24.0	38.0
40-41	34.05879924733451	37.0	34.0	38.0	24.0	38.0
42-43	34.15823657744515	37.0	34.0	38.0	24.0	38.0
44-45	34.16382669182056	37.0	34.0	38.0	24.0	38.0
46-47	34.14971421393894	37.0	34.0	38.0	24.0	38.0
48-49	34.11877163821814	37.0	34.0	38.0	24.0	38.0
50-51	33.99669580826786	37.0	34.0	38.0	24.0	38.0
52-53	34.07945425654771	37.0	34.0	38.0	24.0	38.0
54-55	34.04487578946342	37.0	34.0	38.0	24.0	38.0
56-57	34.01491855676515	37.0	34.0	38.0	24.0	38.0
58-59	34.02280556913925	37.0	34.0	38.0	24.0	38.0
60-61	34.009910136228555	37.0	34.0	38.0	24.0	38.0
62-63	33.97569629484195	37.0	34.0	38.0	24.0	38.0
64-65	33.99224469189129	37.0	34.0	38.0	24.0	38.0
66-67	33.885714233450685	37.0	34.0	38.0	23.0	38.0
68-69	33.70293676122139	37.0	34.0	38.0	22.5	38.0
70-71	33.84768035862584	37.0	34.0	38.0	23.0	38.0
72-73	33.83054112160816	37.0	34.0	38.0	23.0	38.0
74-75	33.84415483055881	37.0	34.0	38.0	23.5	38.0
76	32.647917914097114	37.0	31.0	38.0	21.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	3.0
12	4.0
13	41.0
14	128.0
15	375.0
16	919.0
17	1918.0
18	3218.0
19	4265.0
20	5591.0
21	6598.0
22	8037.0
23	9487.0
24	11355.0
25	13530.0
26	15702.0
27	18557.0
28	21241.0
29	24770.0
30	28928.0
31	33917.0
32	40711.0
33	49160.0
34	60917.0
35	79163.0
36	117383.0
37	264101.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	48.69594485005835	17.88373196230819	9.006498629909796	24.41382455772366
2	23.158731687924305	21.852786337877536	31.53768388293442	23.45079809126374
3	19.354063747303414	22.770569950208472	31.425247463778277	26.450118838709834
4	13.601758008046156	17.467156248818625	37.626201344115195	31.304884399020022
5	13.150548950695045	33.45617601543378	36.96365572017234	16.42961931369883
6	28.334221524135415	38.15594516712418	19.919660398112725	13.590172910627683
7	27.27253880702764	31.519635520640378	22.537770466294074	18.670055206037908
8	24.741134046894036	36.1459917392158	21.002318238967636	18.110555974922534
9	24.44175073992188	17.876658955463228	22.189363904982688	35.4922263996322
10-11	24.087978449279834	25.5380668008912	28.782016026457924	21.591938723371044
12-13	24.6890011085109	23.930908917964096	28.17440815395741	23.205681819567594
14-15	22.521246458923514	25.01777397840782	25.42392310422076	27.037056458447918
16-17	22.41070023987249	28.652019038583255	26.03781131900602	22.899469402538234
18-19	21.635108454804097	28.22300458891806	27.91660924929788	22.22527770697996
20-21	21.905711940820883	28.29318588959524	27.77100286700674	22.030099302577135
22-23	21.873334642246096	27.90849968110495	28.02855787487851	22.189607801770446
24-25	21.95144258852539	27.815209159787763	27.745942472064673	22.48740577962218
26-27	21.610413905043664	27.82453821191948	27.873195621077073	22.691852261959784
28-29	21.554988360025803	27.888622092902725	28.343672524661013	22.212717022410455
30-31	21.787361024561626	28.14946970740922	28.06373998651251	21.999429281516647
32-33	21.395784731817187	28.25641844884082	28.26391827506436	22.08387854427763
34-35	21.850774189378537	27.580702398359065	28.461291750557	22.1072316617054
36-37	22.018270308370905	27.84106221929004	27.766429802236292	22.374237670102765
38-39	21.613035795512054	27.864171439930054	28.325563188170026	22.197229576387866
40-41	21.543098391622635	27.68234638465694	28.69677409913673	22.077781124583698
42-43	21.407430803432604	27.568507558971195	28.576898827953983	22.447162809642215
44-45	21.305664868740845	27.343695694855853	28.680920807932498	22.669718628470804
46-47	21.399199286845793	27.327598506863865	28.67744527870696	22.595756927583384
48-49	20.983721108901136	27.337659249358857	29.07475314596369	22.603866495776316
50-51	21.145180782396505	27.277721613767486	28.96304841717082	22.614049186665184
52-53	20.68982547965352	27.188211492660535	29.375721782056267	22.746241245629676
54-55	20.563060124216634	27.34192744314461	29.32810093424665	22.766911498392112
56-57	20.610548317900726	27.13547402066055	29.284941653648644	22.969036007790084
58-59	20.43095526852846	27.05808609715304	29.302311968493278	23.20864666582522
60-61	20.413577548229274	27.285337899100686	28.970427998007352	23.33065655466269
62-63	20.311359588496085	27.230854501098456	29.0912438957021	23.366542014703363
64-65	20.474966342120155	26.925145363017304	29.101200464381183	23.49868783048136
66-67	20.487831944742542	26.852829700883884	28.800657548145402	23.858680806228172
68-69	20.697863580876984	26.772249843600804	28.547944737731047	23.98194183779117
70-71	21.13796351907124	26.84753679037338	28.187447219114226	23.827052471441156
72-73	20.875019511814404	26.67758921777136	28.352495561062224	24.094895709352013
74-75	21.017734998176866	26.6562156638216	28.14880667108121	24.17724266692032
76	21.083317683270366	26.65539049142824	27.757073625213106	24.50421820008829
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1.0
1	1.0
2	0.5
3	0.0
4	0.0
5	0.0
6	0.5
7	1.0
8	1.0
9	1.5
10	4.0
11	12.5
12	19.0
13	20.5
14	52.0
15	128.5
16	270.5
17	366.0
18	531.0
19	918.0
20	1506.0
21	1872.0
22	2397.5
23	3641.5
24	5295.5
25	6231.0
26	7110.0
27	9152.5
28	11695.0
29	13074.0
30	14648.5
31	17776.0
32	21104.5
33	22880.0
34	24997.0
35	28649.5
36	32283.0
37	34381.0
38	36160.0
39	39508.0
40	42764.0
41	44555.5
42	44660.0
43	44563.5
44	43914.5
45	42947.5
46	42533.0
47	41205.0
48	38227.5
49	34920.0
50	33262.0
51	31460.5
52	27844.5
53	24573.5
54	23117.0
55	21970.5
56	19590.0
57	17014.5
58	15673.0
59	14230.5
60	11722.5
61	9720.0
62	8783.0
63	7975.5
64	6516.5
65	5464.5
66	4701.5
67	4339.0
68	4120.0
69	3607.0
70	3098.5
71	2884.0
72	2718.5
73	2380.5
74	2112.5
75	2017.0
76	1845.0
77	1552.5
78	1251.5
79	1071.0
80	988.5
81	812.5
82	627.5
83	536.0
84	429.0
85	276.0
86	182.0
87	134.0
88	103.5
89	56.5
90	36.0
91	22.0
92	12.0
93	14.5
94	12.5
95	7.0
96	6.0
97	4.5
98	2.5
99	5.5
100	9.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	2.4389678775735686E-4
58-59	4.268193785753745E-4
60-61	4.268193785753745E-4
62-63	3.0487098469669604E-4
64-65	3.6584518163603526E-4
66-67	3.6584518163603526E-4
68-69	2.4389678775735686E-4
70-71	1.8292259081801763E-4
72-73	3.6584518163603526E-4
74-75	2.4389678775735686E-4
76	1.2194839387867843E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	820019.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	64.59693330368023
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.03445612032976	47.17801890875503
2	17.177187220828703	22.191872344974
3	5.796631660654661	11.233338863079307
4	1.931405934019898	4.990516012088622
5	0.6268769077343667	2.024716289926709
6	0.2859228075487175	1.1081841917535302
7	0.18055720529053637	0.8164409223356175
8	0.13773968397302075	0.7118048943100171
9	0.11579950995233021	0.6732263898890576
>10	0.6922480224258306	8.171211737602778
>50	0.020418810038557476	0.8419359155245063
>100	7.561172036081307E-4	0.05873352976085167
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	7.316903632720705E-4	0.0
2	0.0	0.0	0.0	7.316903632720705E-4	0.0
3	0.0	0.0	0.0	8.53638757150749E-4	0.0
4	0.0	0.0	0.0	0.001463380726544141	0.0
5	0.0	0.0	0.0	0.001463380726544141	0.0
6	0.0	0.0	0.0	0.0015853291204228194	0.0
7	0.0	0.0	0.0	0.0015853291204228194	0.0
8	0.0	0.0	0.0	0.001707277514301498	0.0
9	0.0	0.0	0.0	0.002073122695937533	0.0
10	0.0	0.0	0.0	0.00231701948369489	0.0
11	0.0	0.0	0.0	0.00231701948369489	0.0
12	0.0	0.0	0.0	0.00231701948369489	0.0
13	0.0	0.0	0.0	0.00231701948369489	0.0
14	0.0	0.0	0.0	0.0024389678775735683	0.0
15	0.0	0.0	0.0	0.002560916271452247	0.0
16	0.0	0.0	0.0	0.0026828646653309254	0.0
17	0.0	0.0	0.0	0.0026828646653309254	0.0
18	0.0	0.0	0.0	0.0028048130592096036	0.0
19	0.0	0.0	0.0	0.002926761453088282	0.0
20	0.0	0.0	0.0	0.0036584518163603526	0.0
21	0.0	0.0	0.0	0.0039023486041177093	0.0
22	0.0	0.0	0.0	0.00463403896738978	0.0
23	0.0	0.0	0.0	0.004877935755147137	0.0
24	0.0	0.0	0.0	0.005853522906176564	0.0
25	0.0	0.0	0.0	0.006463264875569957	0.0
26	0.0	0.0	0.0	0.007073006844963348	0.0
27	0.0	0.0	0.0	0.010975355449081058	0.0
28	0.0	0.0	0.0	0.01829225908180176	0.0
29	0.0	0.0	0.0	0.030487098469669606	0.0
30	0.0	0.0	0.0	0.03829179567790503	0.0
31	0.0	0.0	0.0	0.05048663506577287	0.0
32	0.0	0.0	0.0	0.06475459714957824	0.0
33	0.0	0.0	0.0	0.08219321747422925	0.0
34	0.0	0.0	0.0	0.11121693521735472	0.0
35	0.0	0.0	0.0	0.15219159556059067	0.0
36	0.0	0.0	0.0	0.2158486571652608	0.0
37	0.0	0.0	0.0	0.3214559662641963	0.0
38	0.0	0.0	0.0	0.4881594206963497	0.0
39	0.0	0.0	0.0	0.7013252131962796	0.0
40	0.0	0.0	0.0	0.9618069825211367	0.0
41	0.0	0.0	0.0	1.2238740809664166	0.0
42	0.0	0.0	0.0	1.4928922378627811	0.0
43	0.0	0.0	0.0	1.805080126192198	0.0
44	0.0	0.0	0.0	2.1381211898748687	0.0
45	0.0	0.0	0.0	2.5143319849905916	0.0
46	0.0	0.0	0.0	2.9432244862619035	0.0
47	0.0	0.0	0.0	3.426993764778621	0.0
48	0.0	0.0	0.0	3.936981947979254	0.0
49	0.0	0.0	0.0	4.479042558769979	0.0
50	0.0	0.0	0.0	5.027688382830154	0.0
51	0.0	0.0	0.0	5.51901846176735	0.0
52	0.0	0.0	0.0	6.007787624433092	0.0
53	0.0	0.0	0.0	6.468752553294497	0.0
54	0.0	0.0	0.0	6.892401273629026	0.0
55	0.0	0.0	0.0	7.340561621133169	0.0
56	0.0	0.0	0.0	7.802136291963967	0.0
57	0.0	0.0	0.0	8.2566379559498	0.0
58	0.0	0.0	0.0	8.745041273433909	0.0
59	0.0	0.0	0.0	9.225030151740386	0.0
60	0.0	0.0	0.0	9.766237123773962	0.0
61	0.0	0.0	0.0	10.261225654527516	0.0
62	0.0	0.0	0.0	10.708898208456146	0.0
63	0.0	0.0	0.0	11.160229214201134	0.0
64	0.0	0.0	0.0	11.580707276294818	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTATCG	40	0.0024190303	35.0	35
TTACGTC	60	4.983062E-4	29.166666	33
CGCGGTA	50	0.007209182	28.000002	43
ATCGATA	50	0.007209182	28.000002	23
AATCGAT	75	5.586145E-5	28.0	22
TCGTTCG	65	7.9573813E-4	26.923077	24
CGAAACG	65	7.9573813E-4	26.923077	15
CGTGATA	70	0.0012262547	25.000002	24
CGATTGA	80	0.0026665016	21.875	54
GGTCCTA	200	1.0677468E-9	21.000002	1
CGAAAGG	85	0.0037898524	20.588236	56
GCGTTGG	85	0.0037898524	20.588236	50
CGTCAGA	125	7.8228695E-5	19.6	2
TCCCGCG	90	0.005275602	19.444443	2
TCGAGTG	90	0.005275602	19.444443	40
GCTATAA	275	7.2759576E-12	19.090908	1
GGTATTA	295	1.8189894E-12	18.983051	1
GTATAAG	410	0.0	18.780487	1
GCGGATG	150	1.4956189E-5	18.666666	13
CGAATCT	730	0.0	18.219177	69
>>END_MODULE