FastQCFastQC Report
Wed 25 May 2016
SRR1781210_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781210_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences732849
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCCGT25230.3442728311016321No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTCT4750.053.05263546
CGTATGC4800.052.541
TATGCCG4800.052.543
TCGTATG4900.051.42857440
CTCGTAT4700.049.8936239
CCGTCTT5250.048.047
CGTCTTC5200.047.7884648
TCTCGTA4450.046.40449538
ATGCCGT5450.046.23853344
TGCCGTC5400.046.0185245
ATCTCGT4450.045.61797737
CGCAGAT4800.044.47916432
GTATGCC5800.043.44827742
TCGCAGA5950.042.35294331
GATCTCG5350.037.94392436
CCTCGCA7150.035.73426429
CTCGCAG7350.035.23809430
AGATCTC6200.032.74193635
GCAGATC6250.031.91999833
CCCTCGC8450.031.06508628
Produced by FastQC (version 0.11.5)