##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1781210_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	732849
Sequences flagged as poor quality	0
Sequence length	76
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.31217617817586	34.0	34.0	34.0	33.0	34.0
2	33.36330403671152	34.0	34.0	34.0	33.0	34.0
3	33.40605090543891	34.0	34.0	34.0	34.0	34.0
4	33.45514833205749	34.0	34.0	34.0	34.0	34.0
5	33.472937808470775	34.0	34.0	34.0	34.0	34.0
6	36.95029944777164	38.0	37.0	38.0	35.0	38.0
7	36.868867938688595	38.0	37.0	38.0	34.0	38.0
8	36.64318297493754	38.0	37.0	38.0	34.0	38.0
9	36.60897674691512	38.0	37.0	38.0	34.0	38.0
10-11	36.53927412058964	38.0	37.0	38.0	34.0	38.0
12-13	36.489400954357585	38.0	37.0	38.0	34.0	38.0
14-15	36.50453981652428	38.0	37.0	38.0	34.0	38.0
16-17	36.46013503463878	38.0	37.0	38.0	34.0	38.0
18-19	36.46545127304533	38.0	37.0	38.0	34.0	38.0
20-21	36.39990502818452	38.0	37.0	38.0	34.0	38.0
22-23	36.45435962933701	38.0	37.0	38.0	34.0	38.0
24-25	36.455660715918285	38.0	37.0	38.0	34.0	38.0
26-27	36.272165889562515	38.0	37.0	38.0	34.0	38.0
28-29	36.25248789314033	38.0	37.0	38.0	34.0	38.0
30-31	36.27800474586169	38.0	37.0	38.0	34.0	38.0
32-33	36.23956572227021	38.0	37.0	38.0	34.0	38.0
34-35	36.247383840327274	38.0	37.0	38.0	34.0	38.0
36-37	36.216020626350044	38.0	37.0	38.0	34.0	38.0
38-39	36.21838264089874	38.0	37.0	38.0	34.0	38.0
40-41	36.20825094937702	38.0	37.0	38.0	34.0	38.0
42-43	36.20363130740439	38.0	37.0	38.0	34.0	38.0
44-45	36.17099566213504	38.0	37.0	38.0	34.0	38.0
46-47	36.189191770746774	38.0	37.0	38.0	34.0	38.0
48-49	36.177140857120634	38.0	37.0	38.0	34.0	38.0
50-51	36.10474668042121	38.0	37.0	38.0	33.5	38.0
52-53	36.13365713810075	38.0	37.0	38.0	34.0	38.0
54-55	36.14203676337144	38.0	37.0	38.0	34.0	38.0
56-57	36.087196680352974	38.0	37.0	38.0	33.0	38.0
58-59	36.10709504959412	38.0	37.0	38.0	34.0	38.0
60-61	36.10621082924313	38.0	37.0	38.0	33.5	38.0
62-63	36.081106749139316	38.0	37.0	38.0	33.5	38.0
64-65	36.034850972028345	38.0	37.0	38.0	32.0	38.0
66-67	36.03582593412831	38.0	37.0	38.0	32.0	38.0
68-69	36.0027079248249	38.0	37.0	38.0	32.0	38.0
70-71	35.96548402194722	38.0	37.0	38.0	32.0	38.0
72-73	35.93821169163088	38.0	37.0	38.0	32.0	38.0
74-75	35.94361253136731	38.0	37.0	38.0	32.0	38.0
76	35.18657731674602	38.0	36.0	38.0	27.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	2.0
19	2.0
20	7.0
21	29.0
22	66.0
23	153.0
24	349.0
25	765.0
26	1458.0
27	2628.0
28	4615.0
29	7630.0
30	12047.0
31	18556.0
32	27137.0
33	38927.0
34	54251.0
35	77688.0
36	123651.0
37	362887.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	47.724428906909885	18.310729768342455	9.383924928600571	24.580916396147092
2	23.36211143086775	22.46724768676767	30.792837269341977	23.377803613022603
3	19.932755588122518	23.34014237585096	30.84455324357405	25.88254879245247
4	13.74307667746016	17.979829405511914	37.48725863035905	30.789835286668875
5	13.068585752317327	34.16147118983584	36.78056461835931	15.989378439487535
6	28.783828592247517	38.64479585835554	19.3334506835651	13.237924865831843
7	27.128098694273987	31.8646815373972	22.219174754963163	18.788045013365647
8	24.5245609941475	37.04228292595064	20.398608717484777	18.034547362417086
9	24.297501941054705	17.87530582698482	21.83492097280613	35.99227125915434
10-11	23.700584977259982	26.148838300932386	28.576487107166688	21.574089614640943
12-13	24.484580043092098	24.34676174764515	28.082865638078243	23.085792571184513
14-15	22.29947779146864	25.036194359274553	25.31776668863572	27.346561160621086
16-17	22.52899301220306	29.007066940119998	25.872724121885955	22.59121592579099
18-19	21.80619745677486	28.384564896724974	27.61892286132614	22.190314785174024
20-21	21.80155802900734	28.426456200390533	27.562840366842284	22.209145403759848
22-23	21.66831093444898	28.349086919679223	27.887054495537278	22.095547650334517
24-25	22.262430596207405	28.078157983431783	27.497342563065512	22.162068857295296
26-27	21.748955105349125	27.975544757514847	27.80170266999068	22.473797467145346
28-29	21.4280840937219	27.859968424600428	28.276357066735443	22.435590414942233
30-31	21.672541000942896	28.045067947148734	28.127144882506492	22.15524616940188
32-33	21.59653625781027	28.345402668216778	28.00228969405703	22.055771379915917
34-35	21.81820538746727	27.889305982542105	28.1449520979083	22.147536532082327
36-37	22.11376422700993	28.221639109830264	27.659449627413014	22.00514703574679
38-39	21.474546598276042	28.1219596397075	28.337215442744686	22.066278319271774
40-41	21.463016255736175	27.617763004384262	28.738253037119517	22.18096770276005
42-43	21.61993807728468	27.62751944807184	28.641916684064523	22.110625790578958
44-45	21.285694597386364	27.683874850071433	28.753262950485027	22.277167602057176
46-47	21.52469335429263	27.427614692794833	28.548786994319432	22.49890495859311
48-49	21.12249590297592	27.266189897236675	28.91113994833861	22.7001742514488
50-51	20.93760106106442	27.226686534333812	29.182682926496454	22.653029478105314
52-53	20.63494662611261	27.132124080131103	29.710349608173036	22.522579685583253
54-55	20.410343740661446	27.343627404826915	29.477286589734035	22.7687422647776
56-57	20.554302455212465	27.166373973356038	29.034289464814716	23.245034106616778
58-59	20.161724993825466	26.970767511451882	29.642463863633573	23.22504363108908
60-61	20.407000623593675	27.51808353426149	28.791742910203876	23.28317293194096
62-63	19.96298009549034	27.71484985310753	28.800134816312774	23.522035235089355
64-65	19.864460482309454	27.614419887316487	28.56980087303114	23.951318757342918
66-67	19.828027328958626	27.526953028522925	28.27171763896792	24.373302003550528
68-69	20.06859530408038	27.61796768502106	28.02657846295758	24.28685854794098
70-71	20.411640051361193	27.519925659992712	27.544487336409002	24.52394695223709
72-73	20.216852312004246	27.147475127891283	27.558678527227297	25.07699403287717
74-75	20.231521090975086	26.982570761507485	27.311970133001477	25.47393801451595
76	20.232817401674833	27.08798128946072	27.11418041097143	25.565020897893014
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.5
10	2.0
11	8.0
12	13.0
13	14.0
14	35.5
15	98.0
16	236.5
17	333.0
18	482.5
19	845.5
20	1502.5
21	1946.0
22	2317.5
23	3323.0
24	4779.0
25	5601.0
26	6682.0
27	8742.5
28	10837.5
29	11953.0
30	13348.5
31	16229.0
32	19462.5
33	21211.0
34	22792.5
35	25707.5
36	28829.5
37	30618.0
38	32049.5
39	35041.0
40	37734.0
41	38976.5
42	39086.0
43	39308.0
44	39141.0
45	38389.0
46	38026.0
47	37100.0
48	34384.0
49	30988.0
50	29382.0
51	27643.0
52	24312.5
53	21845.0
54	20969.0
55	19764.0
56	17527.0
57	15212.0
58	13929.0
59	12782.0
60	10484.5
61	8545.5
62	7757.0
63	6894.5
64	5682.0
65	4959.5
66	4237.5
67	3888.0
68	3589.0
69	3161.5
70	2813.5
71	2594.0
72	2412.5
73	2183.5
74	1968.5
75	1801.0
76	1675.0
77	1459.5
78	1220.5
79	1071.0
80	919.5
81	688.5
82	509.0
83	409.0
84	365.0
85	248.0
86	132.5
87	90.0
88	94.5
89	68.5
90	33.0
91	18.5
92	9.0
93	8.0
94	3.5
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	732849.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.252311574820595
#Duplication Level	Percentage of deduplicated	Percentage of total
1	64.86562790810608	37.78572767399373
2	22.30224127401243	25.98314215021192
3	7.734866388135784	13.517215404938787
4	2.442337650736074	5.690872552063725
5	0.8586084017332388	2.500796206926168
6	0.3612419204168485	1.2625906141205283
7	0.22466870834556432	0.9161230119760815
8	0.17277892841745282	0.8051817577389668
9	0.12673477157175939	0.6644334060865658
>10	0.8807226153474115	9.327563456338329
>50	0.028300409421131812	1.0321865593174888
>100	0.0016371457870937023	0.17043539362608978
>500	0.0	0.0
>1k	2.3387796914357633E-4	0.3437318126616259
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCCGT	2523	0.3442728311016321	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	6.822687893413241E-4	0.0
2	0.0	0.0	0.0	6.822687893413241E-4	0.0
3	0.0	0.0	0.0	8.187225472095889E-4	0.0
4	0.0	0.0	0.0	0.001500991336550913	0.0
5	0.0	0.0	0.0	0.001500991336550913	0.0
6	0.0	0.0	0.0	0.0016374450944191777	0.0
7	0.0	0.0	0.0	0.0016374450944191777	0.0
8	0.0	0.0	0.0	0.0017738988522874425	0.0
9	0.0	0.0	0.0	0.002319713883760502	0.0
10	0.0	0.0	0.0	0.0024561676416287666	0.0
11	0.0	0.0	0.0	0.0024561676416287666	0.0
12	0.0	0.0	0.0	0.0024561676416287666	0.0
13	0.0	0.0	0.0	0.0024561676416287666	0.0
14	0.0	0.0	0.0	0.0025926213994970316	1.364537578682648E-4
15	0.0	0.0	0.0	0.0027290751573652962	1.364537578682648E-4
16	0.0	0.0	0.0	0.0027290751573652962	1.364537578682648E-4
17	1.364537578682648E-4	0.0	0.0	0.0027290751573652962	1.364537578682648E-4
18	1.364537578682648E-4	0.0	0.0	0.0027290751573652962	1.364537578682648E-4
19	1.364537578682648E-4	0.0	0.0	0.0028655289152335612	1.364537578682648E-4
20	1.364537578682648E-4	0.0	0.0	0.003547797704574885	1.364537578682648E-4
21	1.364537578682648E-4	0.0	0.0	0.00368425146244315	1.364537578682648E-4
22	1.364537578682648E-4	0.0	0.0	0.0038207052203114147	1.364537578682648E-4
23	1.364537578682648E-4	0.0	0.0	0.0045029740096527385	1.364537578682648E-4
24	1.364537578682648E-4	0.0	0.0	0.004912335283257533	1.364537578682648E-4
25	1.364537578682648E-4	0.0	0.0	0.005594604072598857	1.364537578682648E-4
26	1.364537578682648E-4	0.0	0.0	0.006276872861940182	1.364537578682648E-4
27	1.364537578682648E-4	0.0	0.0	0.010506939355856391	1.364537578682648E-4
28	1.364537578682648E-4	0.0	0.0	0.018557711070084016	1.364537578682648E-4
29	1.364537578682648E-4	0.0	0.0	0.03220308685691049	1.364537578682648E-4
30	1.364537578682648E-4	0.0	0.0	0.041345488634084236	1.364537578682648E-4
31	1.364537578682648E-4	0.0	0.0	0.05526377193664725	1.364537578682648E-4
32	1.364537578682648E-4	0.0	0.0	0.0709559540914977	1.364537578682648E-4
33	1.364537578682648E-4	0.0	0.0	0.0903323877087913	1.364537578682648E-4
34	1.364537578682648E-4	0.0	0.0	0.12171675201849222	1.364537578682648E-4
35	1.364537578682648E-4	0.0	0.0	0.16565486205207347	1.364537578682648E-4
36	1.364537578682648E-4	0.0	0.0	0.23661081614357118	1.364537578682648E-4
37	1.364537578682648E-4	0.0	0.0	0.3575088456148538	1.364537578682648E-4
38	1.364537578682648E-4	0.0	0.0	0.5432224100735622	1.364537578682648E-4
39	1.364537578682648E-4	0.0	0.0	0.7772406048176363	1.364537578682648E-4
40	1.364537578682648E-4	0.0	0.0	1.060927967425759	1.364537578682648E-4
41	1.364537578682648E-4	0.0	0.0	1.3504828416222168	1.364537578682648E-4
42	1.364537578682648E-4	0.0	0.0	1.6444042360704592	1.364537578682648E-4
43	1.364537578682648E-4	0.0	0.0	1.9839011856467021	1.364537578682648E-4
44	1.364537578682648E-4	0.0	0.0	2.343866198903185	1.364537578682648E-4
45	1.364537578682648E-4	0.0	0.0	2.75472846384453	1.364537578682648E-4
46	1.364537578682648E-4	0.0	0.0	3.2162150729550016	1.364537578682648E-4
47	1.364537578682648E-4	0.0	0.0	3.728462479992468	1.364537578682648E-4
48	4.0936127360479443E-4	0.0	0.0	4.272776520128976	1.364537578682648E-4
49	4.0936127360479443E-4	0.0	0.0	4.849157193364527	1.364537578682648E-4
50	4.0936127360479443E-4	0.0	0.0	5.434407360861514	1.364537578682648E-4
51	4.0936127360479443E-4	0.0	0.0	5.950202565603555	1.364537578682648E-4
52	4.0936127360479443E-4	0.0	0.0	6.471728828176063	1.364537578682648E-4
53	4.0936127360479443E-4	0.0	0.0	6.957367752429218	1.364537578682648E-4
54	4.0936127360479443E-4	0.0	0.0	7.412850396193486	1.364537578682648E-4
55	4.0936127360479443E-4	0.0	0.0	7.888801103637993	1.364537578682648E-4
56	4.0936127360479443E-4	0.0	0.0	8.380443993237352	1.364537578682648E-4
57	4.0936127360479443E-4	0.0	0.0	8.879318932003727	1.364537578682648E-4
58	4.0936127360479443E-4	0.0	0.0	9.400435833302632	1.364537578682648E-4
59	4.0936127360479443E-4	0.0	0.0	9.92742024618987	1.364537578682648E-4
60	4.0936127360479443E-4	0.0	0.0	10.49670532401627	1.364537578682648E-4
61	4.0936127360479443E-4	0.0	0.0	11.018777401620252	1.364537578682648E-4
62	4.0936127360479443E-4	0.0	0.0	11.490634496328711	1.364537578682648E-4
63	4.0936127360479443E-4	0.0	0.0	11.970133001477794	1.364537578682648E-4
64	4.0936127360479443E-4	0.0	0.0	12.416609697222757	1.364537578682648E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCCGTCT	475	0.0	53.052635	46
CGTATGC	480	0.0	52.5	41
TATGCCG	480	0.0	52.5	43
TCGTATG	490	0.0	51.428574	40
CTCGTAT	470	0.0	49.89362	39
CCGTCTT	525	0.0	48.0	47
CGTCTTC	520	0.0	47.78846	48
TCTCGTA	445	0.0	46.404495	38
ATGCCGT	545	0.0	46.238533	44
TGCCGTC	540	0.0	46.01852	45
ATCTCGT	445	0.0	45.617977	37
CGCAGAT	480	0.0	44.479164	32
GTATGCC	580	0.0	43.448277	42
TCGCAGA	595	0.0	42.352943	31
GATCTCG	535	0.0	37.943924	36
CCTCGCA	715	0.0	35.734264	29
CTCGCAG	735	0.0	35.238094	30
AGATCTC	620	0.0	32.741936	35
GCAGATC	625	0.0	31.919998	33
CCCTCGC	845	0.0	31.065086	28
>>END_MODULE