##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1781209_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	31966
Sequences flagged as poor quality	0
Sequence length	76
%GC	60
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.89532628417694	34.0	23.0	34.0	12.0	34.0
2	28.351811299505727	34.0	23.0	34.0	12.0	34.0
3	28.136050803979227	34.0	23.0	34.0	11.0	34.0
4	28.377526121504097	34.0	23.0	34.0	11.0	34.0
5	28.289401238816243	34.0	23.0	34.0	11.0	34.0
6	30.155289995620347	36.0	24.0	38.0	11.0	38.0
7	30.29559532002753	36.0	24.0	38.0	12.0	38.0
8	29.89726584496027	35.0	23.0	38.0	11.0	38.0
9	30.410529938059188	36.0	25.0	38.0	11.0	38.0
10-11	30.063176500031283	35.5	24.0	38.0	11.0	38.0
12-13	30.033254082462616	35.5	23.5	38.0	11.0	38.0
14-15	29.881608584120627	35.0	23.5	38.0	11.0	38.0
16-17	29.77483889132203	35.0	23.0	38.0	11.0	38.0
18-19	29.771413376712758	35.0	23.0	38.0	11.0	38.0
20-21	29.73169930551211	34.5	23.0	38.0	11.0	38.0
22-23	29.688278170556217	34.5	23.0	38.0	11.0	38.0
24-25	29.664127510479886	34.5	23.0	38.0	11.0	38.0
26-27	29.567665644747546	34.0	22.5	38.0	11.0	38.0
28-29	29.650128261277608	34.5	23.0	38.0	11.0	38.0
30-31	29.651833197772632	34.5	23.0	38.0	11.0	38.0
32-33	29.689326159043986	34.5	23.0	38.0	11.0	38.0
34-35	29.690859037727584	34.5	23.0	38.0	11.0	38.0
36-37	29.504848901958333	34.0	22.5	38.0	11.0	38.0
38-39	29.510104486016395	34.0	23.0	38.0	11.0	38.0
40-41	29.51448413939811	34.0	22.5	38.0	11.0	38.0
42-43	29.52593380466746	34.0	23.0	38.0	11.0	38.0
44-45	29.63425201776888	34.5	23.0	38.0	11.0	38.0
46-47	29.582009009572673	34.5	23.0	38.0	11.0	38.0
48-49	29.483419883626354	34.0	22.5	38.0	11.0	38.0
50-51	29.38645748607896	34.0	22.0	38.0	11.0	38.0
52-53	29.406901082400047	34.0	22.0	38.0	11.0	38.0
54-55	29.387802665331918	34.0	22.0	38.0	11.0	38.0
56-57	29.30529312394419	34.0	22.0	38.0	11.0	38.0
58-59	29.208956391165614	34.0	22.0	38.0	11.0	38.0
60-61	29.252142901833196	34.0	22.0	38.0	11.0	38.0
62-63	29.16656760307827	34.0	22.0	38.0	11.0	38.0
64-65	29.13534693111431	34.0	22.0	38.0	11.0	38.0
66-67	29.009525746105236	34.0	22.0	38.0	11.0	38.0
68-69	28.701589188512795	34.0	21.0	38.0	11.0	38.0
70-71	28.78012575861853	34.0	21.5	38.0	11.0	38.0
72-73	28.8158668585372	34.0	21.0	38.0	11.0	38.0
74-75	28.706891697428517	34.0	21.0	38.0	11.0	38.0
76	27.166051429643996	31.0	19.0	37.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	8.0
13	27.0
14	122.0
15	347.0
16	817.0
17	1294.0
18	1668.0
19	1564.0
20	1273.0
21	1004.0
22	711.0
23	650.0
24	657.0
25	617.0
26	689.0
27	770.0
28	884.0
29	895.0
30	1074.0
31	1233.0
32	1362.0
33	1615.0
34	1882.0
35	2261.0
36	3158.0
37	5383.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.78814990927861	15.991991490959146	10.680097603703935	31.539760996058313
2	22.661577926546954	16.39241694300194	30.763936682725397	30.182068447725708
3	23.60633172746043	15.206782206093974	24.319589563911656	36.86729650253394
4	19.495714196333605	12.938747419132829	24.604267033723332	42.961271350810236
5	20.021272602139774	24.698116749045862	28.968278796220986	26.312331852593378
6	37.924669961834454	22.93061377713821	14.884564850153287	24.26015141087405
7	33.616968028530316	20.22774197584934	17.72821122442595	28.42707877119439
8	27.32590877807671	26.521929550147032	19.020208972032783	27.13195269974348
9	24.32897453544391	13.295376337358444	17.994118751173122	44.38153037602453
10-11	30.656009510104486	18.128636676468748	21.45873740849653	29.756616404930234
12-13	28.251892635925667	16.223487455421385	21.693361696802853	33.83125821185009
14-15	27.153850966652065	16.791278233122693	19.084339610836516	36.97053118938873
16-17	28.513107676906714	18.593192767315273	19.728774322717886	33.164925233060124
18-19	28.362948132390663	17.551460927235187	20.629731589814178	33.45585935055997
20-21	28.400488018519678	18.830945379465682	20.058812488268785	32.70975411374585
22-23	28.777451041731837	18.28818119251705	19.874241381467808	33.0601263842833
24-25	28.605393230307204	17.761058624788838	19.239191641118687	34.394356503785275
26-27	28.197146968654195	18.148970781455294	20.687605580929738	32.966276668960774
28-29	28.208096102108488	18.12394419070262	20.426390539948695	33.24156916724019
30-31	28.219045235562785	17.700056309829193	20.03222173559407	34.048676719013955
32-33	27.79515735468936	18.00663204654946	20.467058749921794	33.73115184883939
34-35	28.768066070199588	17.933116436213478	20.556215979478196	32.742601514108735
36-37	28.847838328223734	16.778764937746356	20.426390539948695	33.94700619408121
38-39	27.03653882249891	16.719326784708752	22.41756866670838	33.82656572608396
40-41	28.10016892948758	16.91953951073015	22.99474441594194	31.985547143840332
42-43	26.417130701370205	16.50816492523306	22.356566351748732	34.718138021647995
44-45	25.747669398736157	16.806919852343114	23.845648501532878	33.59976224738785
46-47	26.035475192391917	17.565538384533568	23.454608021022334	32.94437840205218
48-49	25.933804667459174	17.585872489520117	22.80078833760871	33.679534505412
50-51	25.52555840580617	18.386723393605706	22.76794093724582	33.3197772633423
52-53	25.28780579365576	18.536882938121753	23.11831320778327	33.05699806043922
54-55	25.19395607833323	17.599949946818494	22.88838140524307	34.317712569605206
56-57	25.240880935994493	18.657323406119	23.004129387474194	33.09766627041231
58-59	25.48801851967716	18.308515297503597	22.860226490646312	33.34323969217293
60-61	25.886879809797907	17.662516423700183	22.270537446036414	34.1800663204655
62-63	25.067258962647816	18.075455171119316	22.81486579490709	34.042420071325786
64-65	26.033911030469874	17.803290996683977	22.098479634611774	34.06431833823437
66-67	25.77269598948883	18.455546518175563	21.144340862166054	34.627416630169556
68-69	25.25339423137083	19.78195582806732	21.54789463805293	33.41675530250892
70-71	25.794594256397424	19.602077207032472	21.608896953012575	32.994431583557535
72-73	25.660076331101795	18.444597384721266	21.721516611399615	34.17380967277733
74-75	26.410874053682036	18.357004317086904	22.187636864168176	33.04448476506288
76	26.959894888318836	17.43102045923794	21.019207908402677	34.589876744040545
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.5
11	0.5
12	0.0
13	1.0
14	3.5
15	4.0
16	4.0
17	5.0
18	9.5
19	10.0
20	8.5
21	11.0
22	23.0
23	38.5
24	48.5
25	55.0
26	66.5
27	100.5
28	129.5
29	136.0
30	165.0
31	204.5
32	249.5
33	284.0
34	304.5
35	352.0
36	415.5
37	452.0
38	489.0
39	562.0
40	603.0
41	618.5
42	629.0
43	651.0
44	670.5
45	677.0
46	686.0
47	694.5
48	671.0
49	627.0
50	615.0
51	589.5
52	578.5
53	547.5
54	502.0
55	537.5
56	584.0
57	633.5
58	672.0
59	728.5
60	826.0
61	934.5
62	1002.0
63	1019.0
64	1076.5
65	1206.0
66	1349.5
67	1404.0
68	1397.0
69	1420.5
70	1440.0
71	1429.0
72	1419.0
73	1346.0
74	1261.0
75	1239.0
76	1152.5
77	987.0
78	806.5
79	705.0
80	602.0
81	423.0
82	286.5
83	226.0
84	173.0
85	108.0
86	70.5
87	45.0
88	32.5
89	16.0
90	6.5
91	1.0
92	1.0
93	1.0
94	0.5
95	0.0
96	0.0
97	0.5
98	0.5
99	1.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	31966.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.2562097228305
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.1316919238249	74.28517800162673
2	9.547572744285032	16.2797972846149
3	2.506146112354603	6.409935556528811
4	0.5834220085862106	1.98961396483764
5	0.17979671962719698	0.7664393418006632
6	0.04403184970461968	0.22523931677407247
7	0.007338641617436612	0.04379653381718076
8	0.0	0.0
9	0.0	0.0
>10	0.0	0.0
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0031283238440843394	0.0
2	0.0	0.0	0.0	0.0031283238440843394	0.0
3	0.0	0.0	0.0	0.0031283238440843394	0.0
4	0.0	0.0	0.0	0.0031283238440843394	0.0
5	0.0	0.0	0.0	0.0031283238440843394	0.0
6	0.0	0.0	0.0	0.0031283238440843394	0.0
7	0.0	0.0	0.0	0.0031283238440843394	0.0
8	0.0	0.0	0.0	0.0031283238440843394	0.0
9	0.0	0.0	0.0	0.009384971532253018	0.0
10	0.0	0.0	0.0	0.009384971532253018	0.0
11	0.0	0.0	0.0	0.009384971532253018	0.0
12	0.0	0.0	0.0	0.009384971532253018	0.0
13	0.0	0.0	0.0	0.012513295376337358	0.0
14	0.0	0.0	0.0	0.012513295376337358	0.0
15	0.0	0.0	0.0	0.012513295376337358	0.0
16	0.0	0.0	0.0	0.018769943064506037	0.0
17	0.0	0.0	0.0	0.021898266908590376	0.0
18	0.0	0.0	0.0	0.025026590752674716	0.0031283238440843394
19	0.0	0.0	0.0	0.031283238440843394	0.0031283238440843394
20	0.0	0.0	0.0	0.034411562284927734	0.0031283238440843394
21	0.0	0.0	0.0	0.034411562284927734	0.0031283238440843394
22	0.0	0.0	0.0	0.05630982919351811	0.0031283238440843394
23	0.0	0.0	0.0	0.05630982919351811	0.0031283238440843394
24	0.0	0.0	0.0	0.05943815303760245	0.0031283238440843394
25	0.0	0.0	0.0	0.05943815303760245	0.0031283238440843394
26	0.0	0.0	0.0	0.06256647688168679	0.0031283238440843394
27	0.0	0.0	0.0	0.07507977225802415	0.0031283238440843394
28	0.0	0.0	0.0	0.10323468685478321	0.0031283238440843394
29	0.0	0.0	0.0	0.14077457298379528	0.0031283238440843394
30	0.0	0.0	0.0	0.17831445911280736	0.0031283238440843394
31	0.0	0.0	0.0	0.21585434524181943	0.0031283238440843394
32	0.0	0.0	0.0	0.26277920290308454	0.0031283238440843394
33	0.0	0.0	0.0	0.3847838328223738	0.0031283238440843394
34	0.0	0.0	0.0	0.4942751673653257	0.0031283238440843394
35	0.0	0.0	0.0	0.6882312456985548	0.0031283238440843394
36	0.0	0.0	0.0	1.013576925483326	0.0031283238440843394
37	0.0	0.0	0.0	1.4796971782518926	0.0031283238440843394
38	0.0	0.0	0.0	1.942689107176375	0.0031283238440843394
39	0.0	0.0	0.0	2.41506600763311	0.0031283238440843394
40	0.0	0.0	0.0	2.8592879934930866	0.0031283238440843394
41	0.0	0.0	0.0	3.287868360132641	0.0031283238440843394
42	0.0	0.0	0.0	3.722705374460364	0.0031283238440843394
43	0.0	0.0	0.0	4.141900769567665	0.0031283238440843394
44	0.0	0.0	0.0	4.576737783895389	0.0031283238440843394
45	0.0	0.0	0.0	5.092911218169305	0.0031283238440843394
46	0.0	0.0	0.0	5.643496214728149	0.0031283238440843394
47	0.0	0.0	0.0	6.137771382093474	0.0031283238440843394
48	0.0	0.0	0.0	6.685228054808234	0.0031283238440843394
49	0.0	0.0	0.0	7.204529812926234	0.0031283238440843394
50	0.0	0.0	0.0	7.7488581617969094	0.0031283238440843394
51	0.0	0.0	0.0	8.224363386097728	0.0031283238440843394
52	0.0	0.0	0.0	8.64355878120503	0.0031283238440843394
53	0.0	0.0	0.0	9.090909090909092	0.0031283238440843394
54	0.0	0.0	0.0	9.569542639053996	0.0031283238440843394
55	0.0	0.0	0.0	10.063817806419321	0.0031283238440843394
56	0.0	0.0	0.0	10.504911468435212	0.0031283238440843394
57	0.0	0.0	0.0	10.814615528999562	0.0031283238440843394
58	0.0	0.0	0.0	11.283864105612214	0.0031283238440843394
59	0.0	0.0	0.0	11.74372771069261	0.0031283238440843394
60	0.0	0.0	0.0	12.090971657385973	0.0031283238440843394
61	0.0	0.0	0.0	12.50703872864919	0.0031283238440843394
62	0.0	0.0	0.0	12.829256084589877	0.0031283238440843394
63	0.0	0.0	0.0	13.173371707439154	0.0031283238440843394
64	0.0	0.0	0.0	13.561283864105611	0.0031283238440843394
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAATGTG	15	0.0022051055	70.00001	13
TACCGGA	15	0.0022051055	70.00001	2
TCATCTT	25	2.355906E-4	55.999996	5
AACCCCG	20	0.006890646	52.5	31
TCTTAAA	20	0.006890646	52.5	41
TCGCTCC	20	0.006890646	52.5	23
>>END_MODULE