FastQCFastQC Report
Wed 25 May 2016
SRR1781209_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781209_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29531
Sequences flagged as poor quality0
Sequence length76
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCGT510617.290305103111983No Hit
TCTTATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCG4881.652500761911212No Hit
CTTATACACATCTCCGAGCCCACGAGACAACGCCATACCTCGTATGCCGT2870.9718600792387659No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCC2640.8939758220175408No Hit
CTTATACACATCTCCGAGCCCACGAGACAACGCCATAACTCGTATGCCGT1830.6196877857167045No Hit
ATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCGTCTT1140.38603501405302904Illumina PCR Primer Index 6 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACAACGCCATAGCTCGTATGCCGT980.33185466120348106No Hit
TCTTTATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCC630.21333513934509496No Hit
CTTATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCGC450.15238224238935358No Hit
TCTTATACACATCTCCGAGCCCACGAGACAACGCCATACCTCGTATGCCG400.13545088212386983No Hit
CTATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCGTC360.12190579391148285No Hit
CTTATACACATCTCCGAGCCCACGAGACAACGCCATATTTCGTATGCCGT300.10158816159290236No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAACTCC150.002203419370.068
CCGCTTG207.801469E-570.054
TAGCTCG150.002203419370.036
GCTCGTA150.002203419370.038
AAAACTC309.900941E-870.067
AAAAAGC150.002203419370.066
CATATCT7450.059.66443334
TATCTCG7400.059.59459336
ATATCTC7400.059.59459335
CCATATC7600.059.4078933
CTCGTAT8050.059.13043639
TTCTGCT7650.059.01960852
TCTCGTA7450.058.7248438
CTGCTTG7750.058.70967554
TGCTTGA7650.058.5620955
GCTTGAA7900.058.48101456
ATCTCGT7550.058.41059537
CTTGAAA7850.058.40764657
ATACCTC308.180314E-658.33333235
TACCTCG308.180314E-658.33333236