##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1781209_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	29531
Sequences flagged as poor quality	0
Sequence length	76
%GC	60
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.220717212420844	34.0	34.0	34.0	32.0	34.0
2	33.24743489891978	34.0	34.0	34.0	32.0	34.0
3	33.25153228810402	34.0	34.0	34.0	33.0	34.0
4	33.3721174359148	34.0	34.0	34.0	33.0	34.0
5	33.35884324946666	34.0	34.0	34.0	33.0	34.0
6	36.75314076732925	38.0	37.0	38.0	34.0	38.0
7	36.56648945176256	38.0	37.0	38.0	34.0	38.0
8	36.369137516508076	38.0	37.0	38.0	34.0	38.0
9	36.35257864616843	38.0	37.0	38.0	34.0	38.0
10-11	36.20216044156987	38.0	37.0	38.0	33.5	38.0
12-13	36.08270969489689	38.0	36.0	38.0	32.0	38.0
14-15	36.105245335410245	38.0	36.0	38.0	32.5	38.0
16-17	35.93349361687718	38.0	36.0	38.0	31.0	38.0
18-19	35.845670651180114	38.0	36.0	38.0	31.0	38.0
20-21	35.82445565676746	38.0	36.0	38.0	31.0	38.0
22-23	35.98306863973452	38.0	36.0	38.0	31.5	38.0
24-25	35.88686465070604	38.0	36.0	38.0	31.0	38.0
26-27	35.543953811249196	38.0	36.0	38.0	31.0	38.0
28-29	35.55311367715282	38.0	36.0	38.0	31.0	38.0
30-31	35.71118485659137	38.0	36.0	38.0	31.0	38.0
32-33	35.37902543090311	38.0	35.5	38.0	31.0	38.0
34-35	35.30642375808472	38.0	35.5	38.0	31.0	38.0
36-37	35.1241576648268	38.0	35.0	38.0	29.0	38.0
38-39	33.8173275540957	37.0	34.0	38.0	23.5	38.0
40-41	34.8415563306356	37.0	34.5	38.0	27.5	38.0
42-43	35.3602485523687	38.0	35.5	38.0	31.0	38.0
44-45	35.3325827096949	38.0	35.5	38.0	31.0	38.0
46-47	35.25735667603536	38.0	35.5	38.0	29.0	38.0
48-49	35.12828891673157	38.0	35.5	38.0	28.0	38.0
50-51	34.846364836951	37.0	34.0	38.0	27.0	38.0
52-53	34.82083234567065	38.0	35.0	38.0	27.0	38.0
54-55	34.80334225051641	37.5	35.0	38.0	27.0	38.0
56-57	34.76638109105686	37.0	34.5	38.0	27.0	38.0
58-59	34.48003792624699	37.0	34.0	38.0	25.0	38.0
60-61	34.42518031898683	37.0	34.0	38.0	25.5	38.0
62-63	34.544631065659814	37.0	34.0	38.0	26.0	38.0
64-65	34.491297280823545	37.0	34.0	38.0	25.0	38.0
66-67	34.03807862923708	37.0	34.0	38.0	24.0	38.0
68-69	33.275202329755174	37.0	33.5	38.0	22.0	38.0
70-71	31.67913379160882	37.0	28.0	38.0	15.5	38.0
72-73	30.489790389759914	37.0	24.0	38.0	11.0	38.0
74-75	30.558430124276185	37.0	25.0	38.0	11.0	38.0
76	29.669398259456166	35.0	23.0	38.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
21	3.0
22	11.0
23	28.0
24	82.0
25	143.0
26	273.0
27	430.0
28	697.0
29	876.0
30	1244.0
31	1518.0
32	2018.0
33	2491.0
34	3143.0
35	4019.0
36	3882.0
37	8673.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.66083099116183	10.087704446175206	8.909281771697538	45.342182790965424
2	20.31085977447428	12.407300802546477	42.047340083302295	25.23449933967695
3	20.588534082828215	11.208560495750229	39.060648132470966	29.142257288950596
4	16.257492126917477	30.47644847787071	19.559107378686804	33.70695201652501
5	17.632318580474752	21.763570485252785	41.38701703294843	19.21709390132403
6	32.53868815820663	39.35864007314348	10.930886187396295	17.171785581253598
7	29.132098472791306	17.449459889607528	11.161152687006874	42.257288950594294
8	23.886763062544443	42.99210998611628	11.201787951644034	21.919338999695235
9	21.252243405235177	11.601368053909452	10.460194372015847	56.68619416883952
10-11	27.36954386915445	25.278520876367207	23.29077918119942	24.061156073278926
12-13	24.955131895296468	11.149300734821036	25.75598523585385	38.13958213402865
14-15	23.9341708712878	10.90548914699807	13.120111069723341	52.040228911990795
16-17	37.78063729640039	23.17395279536758	11.682638583183772	27.36277132504825
18-19	37.90762249839152	13.843080153059498	11.515018116555483	36.7342792319935
20-21	25.4986285598185	12.138092174325285	12.068673597236803	50.29460566861942
22-23	25.89143611797772	24.44380481527886	11.730046391927127	37.934712674816296
24-25	37.93640581084284	23.678507331278993	11.550573973113	26.83451288476516
26-27	37.55206393281637	25.539263824455656	12.158409806643867	24.750262436084114
28-29	26.238529003420137	25.20232975517253	11.926450171006739	36.6326910704006
30-31	25.904981206190104	25.8558802614202	11.355863330059938	36.883275202329756
32-33	36.10951203819715	12.720530967457924	12.81873285699773	38.351224137347195
34-35	26.152179066066168	23.461785919880803	12.458094883342929	37.927940130710105
36-37	24.743489891977923	24.479360671836375	24.415021502827535	26.362127933358163
38-39	24.51660966442044	13.915885002201076	24.572483153296538	36.995022180081946
40-41	24.189834411296605	11.868883546104094	26.236835867393587	37.70444617520571
42-43	36.76644881649791	11.277979072838711	24.821374149199148	27.134197961464224
44-45	24.052690393146186	23.754698452473672	26.83451288476516	25.35809826961498
46-47	35.68114862348041	12.556296772882733	16.645220276997055	35.117334326639806
48-49	35.13595882293183	11.697876807422707	13.961599674917883	39.20456469472757
50-51	24.37269310216383	12.453015475263282	26.71260709085368	36.46168433171921
52-53	22.486539568588938	12.41407334665267	38.229318343435715	26.87006874132268
54-55	22.606752226473876	11.657241542785547	28.11621685686228	37.6197893738783
56-57	34.00494395719752	12.412380210626122	17.083742507873083	36.49893332430327
58-59	23.14178321086316	13.008364091971151	36.812163489214726	27.037689207950965
60-61	34.286004537604555	24.061156073278926	16.079712844129897	25.573126544986625
62-63	22.13436727506688	38.72202092716129	13.467203955165758	25.676407842606075
64-65	21.761877349226236	39.06742067657716	13.729640039280754	25.441061934915847
66-67	21.91595272764214	38.950594290745315	12.7967220886526	26.33673089295994
68-69	21.76864989333243	38.83715417696658	13.458738275033017	25.935457654667974
70-71	22.647387491111033	33.6646236158613	15.029968507669905	28.65802038535776
72-73	24.19491381937625	25.148149402322982	18.101317259828654	32.55561951847211
74-75	24.01544140056212	23.085909721987065	19.037621482509905	33.861027394940905
76	24.001896312349736	23.22644001219058	18.766719718262166	34.00494395719752
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	2.0
15	5.5
16	5.0
17	3.0
18	2.0
19	3.0
20	6.5
21	8.0
22	14.5
23	16.5
24	15.0
25	18.0
26	25.0
27	34.0
28	43.5
29	51.0
30	54.0
31	68.5
32	85.0
33	90.0
34	108.0
35	119.5
36	137.5
37	162.0
38	146.0
39	141.5
40	475.5
41	1288.5
42	1779.0
43	1996.5
44	2038.0
45	1516.0
46	1170.0
47	922.0
48	533.5
49	322.5
50	252.0
51	212.0
52	191.0
53	203.5
54	197.0
55	230.0
56	270.5
57	349.5
58	421.0
59	487.5
60	601.0
61	741.0
62	834.0
63	862.5
64	957.0
65	1065.0
66	1145.0
67	1183.0
68	1256.5
69	1357.5
70	1359.5
71	1334.0
72	1339.0
73	1320.0
74	1196.5
75	1097.0
76	1062.5
77	908.0
78	730.5
79	673.0
80	568.0
81	381.0
82	258.0
83	217.0
84	179.5
85	98.0
86	40.0
87	26.0
88	20.0
89	10.5
90	4.0
91	1.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	29531.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.35772577968914
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.65601153638165	42.074430259727066
2	18.235894970858617	20.554671362297245
3	5.011115784413868	8.472452676848057
4	1.3158685333173106	2.966374318512749
5	0.33647779847383286	0.9481561748670888
6	0.12017064231208316	0.40635264637160945
7	0.06008532115604158	0.2370390437167722
8	0.04205972480922911	0.18963123497341777
9	0.018025596346812472	0.09142934543361214
>10	0.1562218350057081	1.6999085706545662
>50	0.012017064231208316	0.545189800548576
>100	0.03004266057802079	4.524059462937252
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.006008532115604158	17.290305103111983
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCGT	5106	17.290305103111983	No Hit
TCTTATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCG	488	1.652500761911212	No Hit
CTTATACACATCTCCGAGCCCACGAGACAACGCCATACCTCGTATGCCGT	287	0.9718600792387659	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCC	264	0.8939758220175408	No Hit
CTTATACACATCTCCGAGCCCACGAGACAACGCCATAACTCGTATGCCGT	183	0.6196877857167045	No Hit
ATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCGTCTT	114	0.38603501405302904	Illumina PCR Primer Index 6 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACAACGCCATAGCTCGTATGCCGT	98	0.33185466120348106	No Hit
TCTTTATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCC	63	0.21333513934509496	No Hit
CTTATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCGC	45	0.15238224238935358	No Hit
TCTTATACACATCTCCGAGCCCACGAGACAACGCCATACCTCGTATGCCG	40	0.13545088212386983	No Hit
CTATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCGTC	36	0.12190579391148285	No Hit
CTTATACACATCTCCGAGCCCACGAGACAACGCCATATTTCGTATGCCGT	30	0.10158816159290236	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.003386272053096746	0.0
8	0.0	0.0	0.0	0.003386272053096746	0.0
9	0.0	0.0	0.0	0.006772544106193492	0.0
10	0.0	0.0	0.0	0.006772544106193492	0.0
11	0.0	0.0	0.0	0.006772544106193492	0.0
12	0.0	0.0	0.0	0.006772544106193492	0.0
13	0.0	0.0	0.0	0.006772544106193492	0.0
14	0.0	0.0	0.0	0.006772544106193492	0.0
15	0.0	0.0	0.0	0.006772544106193492	0.0
16	0.0	0.0	0.0	0.013545088212386983	0.0
17	0.0	0.0	0.0	0.016931360265483728	0.0
18	0.0	0.0	0.0	0.016931360265483728	0.0
19	0.0	0.0	0.0	0.027090176424773967	0.0
20	0.0	0.0	0.0	0.03047644847787071	0.0
21	0.0	0.0	0.0	0.03047644847787071	0.003386272053096746
22	0.0	0.0	0.0	0.03047644847787071	0.003386272053096746
23	0.0	0.0	0.0	0.03047644847787071	0.003386272053096746
24	0.0	0.0	0.0	0.03047644847787071	0.003386272053096746
25	0.0	0.0	0.0	0.033862720530967456	0.003386272053096746
26	0.0	0.0	0.0	0.0372489925840642	0.003386272053096746
27	0.0	0.0	0.0	0.05079408079645119	0.003386272053096746
28	0.0	0.0	0.0	0.08465680132741865	0.003386272053096746
29	0.0	0.0	0.0	0.1252920659645796	0.003386272053096746
30	0.0	0.0	0.0	0.15915478649554704	0.003386272053096746
31	0.0	0.0	0.0	0.2065625952389015	0.003386272053096746
32	0.0	0.0	0.0	0.26412922014154616	0.003386272053096746
33	0.0	0.0	0.0	0.39619383021231924	0.003386272053096746
34	0.0	0.0	0.0	0.5079408079645119	0.003386272053096746
35	0.0	0.0	0.0	0.7246622193627036	0.003386272053096746
36	0.0	0.0	0.0	1.0937658731502489	0.003386272053096746
37	0.0	0.0	0.0	1.598320409061664	0.003386272053096746
38	0.0	0.0	0.0	2.0825573126544987	0.003386272053096746
39	0.0	0.0	0.0	2.56002167214114	0.003386272053096746
40	0.0	0.0	0.0	3.0307134875215875	0.003386272053096746
41	0.0	0.0	0.0	3.4878602146896482	0.003386272053096746
42	0.0	0.0	0.0	3.9483932139108058	0.003386272053096746
43	0.0	0.0	0.0	4.4055399410788665	0.003386272053096746
44	0.0	0.0	0.0	4.852527852087637	0.003386272053096746
45	0.0	0.0	0.0	5.363854932105245	0.003386272053096746
46	0.0	0.0	0.0	5.929362364972402	0.003386272053096746
47	0.0	0.0	0.0	6.44068944499001	0.003386272053096746
48	0.0	0.0	0.0	7.019741966069554	0.003386272053096746
49	0.0	0.0	0.0	7.524296501980969	0.003386272053096746
50	0.0	0.0	0.0	8.099962751007416	0.003386272053096746
51	0.0	0.0	0.0	8.574040838440961	0.003386272053096746
52	0.0	0.0	0.0	9.061664014086892	0.003386272053096746
53	0.0	0.0	0.0	9.545900917679726	0.003386272053096746
54	0.0	0.0	0.0	10.040296637431851	0.003386272053096746
55	0.0	0.0	0.0	10.524533541024686	0.003386272053096746
56	0.0	0.0	0.0	10.981680268192747	0.003386272053096746
57	0.0	0.0	0.0	11.306762385290034	0.003386272053096746
58	0.0	0.0	0.0	11.774067928617384	0.003386272053096746
59	0.0	0.0	0.0	12.285395008634994	0.003386272053096746
60	0.0	0.0	0.0	12.640953574210153	0.003386272053096746
61	0.0	0.0	0.0	13.060851308794149	0.003386272053096746
62	0.0	0.0	0.0	13.436727506687888	0.003386272053096746
63	0.0	0.0	0.0	13.792286072263046	0.003386272053096746
64	0.0	0.0	0.0	14.21557007890014	0.003386272053096746
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAACTCC	15	0.0022034193	70.0	68
CCGCTTG	20	7.801469E-5	70.0	54
TAGCTCG	15	0.0022034193	70.0	36
GCTCGTA	15	0.0022034193	70.0	38
AAAACTC	30	9.900941E-8	70.0	67
AAAAAGC	15	0.0022034193	70.0	66
CATATCT	745	0.0	59.664433	34
TATCTCG	740	0.0	59.594593	36
ATATCTC	740	0.0	59.594593	35
CCATATC	760	0.0	59.40789	33
CTCGTAT	805	0.0	59.130436	39
TTCTGCT	765	0.0	59.019608	52
TCTCGTA	745	0.0	58.72484	38
CTGCTTG	775	0.0	58.709675	54
TGCTTGA	765	0.0	58.56209	55
GCTTGAA	790	0.0	58.481014	56
ATCTCGT	755	0.0	58.410595	37
CTTGAAA	785	0.0	58.407646	57
ATACCTC	30	8.180314E-6	58.333332	35
TACCTCG	30	8.180314E-6	58.333332	36
>>END_MODULE