##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1781208_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	16837
Sequences flagged as poor quality	0
Sequence length	76
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	25.343469739264716	31.0	12.0	34.0	12.0	34.0
2	25.604086238641088	31.0	12.0	34.0	12.0	34.0
3	25.533408564471106	31.0	11.0	34.0	11.0	34.0
4	25.534240066520162	31.0	11.0	34.0	11.0	34.0
5	25.648927956286748	32.0	11.0	34.0	11.0	34.0
6	26.694422996970957	31.0	11.0	37.0	11.0	38.0
7	27.007721090455544	31.0	12.0	37.0	12.0	38.0
8	26.679455960087903	31.0	11.0	37.0	11.0	38.0
9	27.43855793787492	33.0	12.0	37.0	11.0	38.0
10-11	26.99759458335808	31.5	11.0	37.0	11.0	38.0
12-13	26.96691809704817	31.0	11.0	37.0	11.0	38.0
14-15	26.84747876700125	31.0	11.0	37.0	11.0	38.0
16-17	26.525657777513807	31.0	11.0	37.0	11.0	38.0
18-19	26.63868266318228	31.0	11.0	37.0	11.0	38.0
20-21	26.750816653798182	31.0	11.0	37.0	11.0	38.0
22-23	26.6304864286987	31.0	11.0	37.0	11.0	38.0
24-25	26.60764981885134	31.0	11.0	37.0	11.0	38.0
26-27	26.520787551226466	31.0	11.0	37.0	11.0	38.0
28-29	26.568747401556095	31.0	11.0	37.0	11.0	38.0
30-31	26.639781433747103	31.0	11.0	37.0	11.0	38.0
32-33	26.554908831739624	31.0	11.0	37.0	11.0	38.0
34-35	26.551404644532873	31.0	11.0	37.0	11.0	38.0
36-37	26.467571420086713	31.0	11.0	37.0	11.0	38.0
38-39	26.551464037536377	31.0	11.0	37.0	11.0	38.0
40-41	26.500920591554312	31.0	11.0	37.0	11.0	38.0
42-43	26.57741878006771	31.0	11.0	37.0	11.0	38.0
44-45	26.642008671378512	31.0	11.0	37.0	11.0	38.0
46-47	26.51885727861258	31.0	11.0	37.0	11.0	38.0
48-49	26.48892320484647	31.0	11.0	37.0	11.0	38.0
50-51	26.436271307240006	31.0	11.0	37.0	11.0	38.0
52-53	26.42483815406545	31.0	11.0	37.0	11.0	38.0
54-55	26.429322325830015	31.0	11.0	37.0	11.0	38.0
56-57	26.34804300053454	31.0	11.0	37.0	11.0	38.0
58-59	26.310714497832155	31.0	11.0	37.0	11.0	38.0
60-61	26.280780424066045	30.5	11.0	37.0	11.0	38.0
62-63	26.230533943101502	30.0	11.0	37.0	11.0	38.0
64-65	26.191720615311517	30.0	11.0	37.0	11.0	38.0
66-67	26.05300825562749	28.0	11.0	37.0	11.0	38.0
68-69	25.8744728870939	27.0	11.0	37.0	11.0	38.0
70-71	25.887034507335038	27.0	11.0	37.0	11.0	38.0
72-73	25.871711112430955	27.5	11.0	37.0	11.0	38.0
74-75	25.81145691037596	27.5	11.0	37.0	11.0	38.0
76	24.71336936508879	26.0	11.0	36.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	1.0
13	23.0
14	92.0
15	311.0
16	686.0
17	1259.0
18	1617.0
19	1553.0
20	1102.0
21	833.0
22	538.0
23	372.0
24	321.0
25	338.0
26	281.0
27	275.0
28	283.0
29	340.0
30	369.0
31	454.0
32	459.0
33	528.0
34	695.0
35	790.0
36	1182.0
37	2133.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.90241729524262	15.483756013541605	13.007067767416999	32.60675892379878
2	21.446813565362	17.586268337589832	31.5673813624755	29.399536734572667
3	18.37619528419552	16.84979509413791	28.407673576052737	36.36633604561383
4	14.848250876046801	17.63378274039318	29.090693116350895	38.427273267209124
5	15.174912395319831	25.610263111005523	36.229732137554194	22.985092356120447
6	28.003801152224266	25.277662291382075	22.456494624933182	24.262041931460473
7	24.37488863811843	23.20484646908594	24.523371146878898	27.896893745916728
8	22.480251826334857	27.302963710874863	22.076379402506387	28.140405060283896
9	20.502464809645424	14.509710756072936	22.89006355051375	42.09776088376789
10-11	23.041515709449424	20.677674169982776	26.682306824256102	29.598503296311694
12-13	22.468373225634018	18.729583655045438	27.005998693353924	31.79604442596662
14-15	21.990259547425314	20.56779711350003	23.890835659559304	33.551107679515354
16-17	23.21672506978678	21.32208825800321	24.410524440220943	31.050662231989072
18-19	21.241907703272556	21.470570766763675	26.0468016867613	31.24071984320247
20-21	22.438676723881926	21.515115519391816	25.663716814159294	30.382490942566964
22-23	23.056363960325474	21.07857694363604	25.13214943279682	30.73290966324167
24-25	22.18031715863871	21.506206568866187	24.79360931282295	31.51986695967215
26-27	22.233770861792483	21.856625289540894	25.63698996258241	30.272613886084223
28-29	21.987289897250104	21.286452455900694	26.296252301478884	30.430005345370315
30-31	21.529963770267862	21.096394844687296	26.343766704282235	31.029874680762603
32-33	21.660628377977073	21.319118607827996	25.84189582467185	31.178357189523076
34-35	22.590128882817602	20.89742828294827	25.54790045732613	30.964542376908
36-37	22.964304804893985	19.97683672863337	25.663716814159294	31.395141652313356
38-39	22.221892261091643	19.31163508938647	27.876106194690266	30.590366454831617
40-41	22.04668290075429	19.73035576409099	28.78481914830433	29.438142186850392
42-43	20.52028271069668	19.252242085882283	28.683851042347214	31.543624161073826
44-45	19.77490051671913	18.6553424006652	30.617093306408506	30.952663776207164
46-47	20.79646017699115	20.490586208944585	29.114450317752567	29.598503296311694
48-49	20.024351131436717	20.852883530320128	28.615549088317394	30.507216249925754
50-51	20.398527053513096	21.090455544336876	27.305933361050066	31.205084041099955
52-53	19.281938587634375	22.00510779830136	28.282948268693946	30.430005345370315
54-55	19.53732850270238	20.28864999703035	28.0453762546772	32.12864524559007
56-57	20.048108332838392	21.325057908178415	27.84047039258775	30.786363366395435
58-59	20.529191661222306	20.84100492961929	27.356417414028627	31.27338599512977
60-61	19.96198847775732	20.52028271069668	27.19308665439211	32.32464215715389
62-63	20.410405654213935	20.122349587218626	27.15448120211439	32.31276355645305
64-65	20.760824374888635	19.855081071449785	26.726851576884243	32.65724297677733
66-67	20.547009562273562	22.15062065688662	24.918334620181742	32.38403516065807
68-69	21.02809289065748	22.780186494031003	25.06681712894221	31.124903486369305
70-71	19.97683672863337	23.287996673991803	26.017105185009203	30.71806141236562
72-73	20.131258537744255	21.82395913761359	26.049771336936512	31.995010987705648
74-75	22.343647918275227	21.283482805725484	26.09431608956465	30.278553186434635
76	21.179545049593155	20.05701728336402	25.438023400843377	33.325414266199445
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	4.0
16	7.0
17	6.0
18	8.0
19	10.0
20	15.5
21	21.0
22	24.5
23	35.5
24	56.0
25	69.0
26	72.0
27	81.0
28	110.0
29	133.0
30	147.5
31	179.5
32	231.0
33	265.0
34	302.0
35	363.5
36	408.0
37	428.0
38	447.0
39	518.0
40	608.5
41	618.5
42	590.0
43	630.0
44	673.0
45	660.5
46	645.0
47	638.0
48	633.5
49	620.5
50	605.0
51	573.0
52	528.0
53	495.5
54	476.0
55	468.5
56	426.5
57	381.0
58	370.0
59	386.5
60	361.5
61	358.0
62	396.0
63	388.5
64	370.0
65	363.5
66	385.5
67	403.0
68	407.0
69	380.0
70	362.5
71	376.0
72	354.0
73	332.5
74	308.5
75	284.0
76	260.0
77	220.5
78	182.0
79	159.0
80	142.5
81	103.5
82	69.5
83	58.0
84	47.0
85	35.5
86	23.5
87	12.0
88	8.5
89	4.5
90	3.0
91	1.5
92	1.0
93	0.5
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	16837.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	89.8794322028865
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.77955461574044	82.49094256696561
2	6.006740236569088	10.797648037061235
3	1.612370316526796	4.347567856506504
4	0.4097006541994317	1.4729464869038427
5	0.17180995176105202	0.7721090455544336
6	0.013216150135465538	0.07127160420502465
7	0.0	0.0
8	0.006608075067732769	0.047514402803349766
9	0.0	0.0
>10	0.0	0.0
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.005939300350418721	0.0
2	0.0	0.0	0.0	0.005939300350418721	0.0
3	0.0	0.0	0.0	0.005939300350418721	0.0
4	0.0	0.0	0.0	0.011878600700837441	0.0
5	0.0	0.0	0.0	0.011878600700837441	0.0
6	0.0	0.0	0.0	0.011878600700837441	0.0
7	0.0	0.0	0.0	0.017817901051256163	0.0
8	0.0	0.0	0.0	0.017817901051256163	0.0
9	0.0	0.0	0.0	0.017817901051256163	0.0
10	0.0	0.0	0.0	0.023757201401674883	0.0
11	0.0	0.0	0.0	0.023757201401674883	0.0
12	0.0	0.0	0.0	0.023757201401674883	0.0
13	0.0	0.0	0.0	0.023757201401674883	0.0
14	0.0	0.0	0.0	0.023757201401674883	0.0
15	0.0	0.0	0.0	0.023757201401674883	0.0
16	0.0	0.0	0.0	0.023757201401674883	0.0
17	0.0	0.0	0.0	0.023757201401674883	0.0
18	0.0	0.0	0.0	0.047514402803349766	0.0
19	0.0	0.0	0.0	0.047514402803349766	0.0
20	0.0	0.0	0.0	0.047514402803349766	0.0
21	0.0	0.0	0.0	0.047514402803349766	0.0
22	0.0	0.0	0.0	0.059393003504187206	0.0
23	0.0	0.0	0.0	0.06533230385460592	0.0
24	0.0	0.0	0.0	0.09502880560669953	0.0
25	0.0	0.0	0.0	0.09502880560669953	0.0
26	0.0	0.0	0.0	0.09502880560669953	0.0
27	0.0	0.0	0.0	0.12472530735879313	0.0
28	0.0	0.0	0.0	0.15442180911088674	0.0
29	0.0	0.0	0.0	0.1722397101621429	0.0
30	0.0	0.0	0.0	0.2256934133159114	0.0
31	0.0	0.0	0.0	0.255389915068005	0.0
32	0.0	0.0	0.0	0.3266615192730296	0.0
33	0.0	0.0	0.0	0.3801152224267981	0.0
34	0.0	0.0	0.0	0.4810833283839164	0.0
35	0.0	0.0	0.0	0.593930035041872	0.0
36	0.0	0.0	0.0	0.7958662469561085	0.0
37	0.0	0.0	0.0	1.1641028686820694	0.0
38	0.0	0.0	0.0	1.5264001900576112	0.0
39	0.0	0.0	0.0	1.847122408980222	0.0
40	0.0	0.0	0.0	2.185662528954089	0.0
41	0.0	0.0	0.0	2.5420205499792123	0.0
42	0.0	0.0	0.0	2.8568034685514045	0.0
43	0.0	0.0	0.0	3.254736592029459	0.0
44	0.0	0.0	0.0	3.640791114806676	0.0
45	0.0	0.0	0.0	4.13375304389143	0.0
46	0.0	0.0	0.0	4.6504721743778585	0.0
47	0.0	0.0	0.0	5.161252004513869	0.0
48	0.0	0.0	0.0	5.565124428342341	0.0
49	0.0	0.0	0.0	5.957118251469977	0.0
50	0.0	0.0	0.0	6.3134762724951	0.0
51	0.0	0.0	0.0	6.782681000178179	0.0
52	0.0	0.0	0.0	7.168735522955396	0.0
53	0.0	0.0	0.0	7.560729346083032	0.0
54	0.0	0.0	0.0	7.923026667458573	0.0
55	0.0	0.0	0.0	8.30908119023579	0.0
56	0.0	0.0	0.0	8.611985508107145	0.0
57	0.0	0.0	0.0	8.903011225277663	0.0
58	0.0	0.0	0.0	9.253429945952368	0.0
59	0.0	0.0	0.0	9.586030765575815	0.0
60	0.0	0.0	0.0	9.835481380293402	0.0
61	0.0	0.0	0.0	10.144324998515176	0.0
62	0.0	0.0	0.0	10.429411415335274	0.0
63	0.0	0.0	0.0	10.744194333907465	0.0
64	0.0	0.0	0.0	11.09461305458217	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CACAAGA	15	0.0021868346	70.00001	7
AGAGGCT	15	0.0021868346	70.00001	11
CCAGAAG	15	0.0021868346	70.00001	61
GCACCCT	15	0.0021868346	70.00001	13
GTCGATG	20	0.0068339882	52.5	40
GCCGACG	95	0.0069262027	18.421051	69
>>END_MODULE