FastQCFastQC Report
Wed 25 May 2016
SRR1781208_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781208_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15509
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCGT500532.27158424140821No Hit
TCTTATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCG4502.901541040686053No Hit
CTTATACACATCTCCGAGCCCACGAGACGAAATGCCACCTCGTATGCCGT3402.1922754529627957No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCC2291.4765619962602359No Hit
CTTATACACATCTCCGAGCCCACGAGACGAAATGCCAACTCGTATGCCGT2151.3862918305500032No Hit
CTTATACACATCTCCGAGCCCACGAGACGAAATGCCAGCTCGTATGCCGT1060.6834741118060481No Hit
ATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCGTCTT980.6318911599716294RNA PCR Primer, Index 20 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACGAAATGCCATATCGTATGCCGT840.5416209942613966No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCC800.5158295183441872No Hit
TCTTATACACATCTCCGAGCCCACGAGACGAAATGCCACCTCGTATGCCG480.30949771100651235No Hit
CTTATACACATCTCCGAGCCCACGAGACGAAATGCCATTTCGTATGCCGT420.2708104971306983No Hit
TCTTATACACATCTCCGAGCCCACGAGACGAAATGCCAACTCGTATGCCG330.21277967631697725No Hit
CTTATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCGC320.2063318073376749No Hit
CTATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCGTC300.1934360693790702No Hit
CTTATACACATCTCCGAGCCCACGAGACGAAATGCCATCCCGTATGCCGT290.18698820039976788No Hit
CTTTACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCGTC240.15474885550325618No Hit
TCTTAATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCC230.14830098652395385No Hit
CTTTATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCG220.1418531175446515No Hit
CTTATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCGA210.13540524856534916No Hit
TATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCGTCT200.1289573795860468No Hit
CCTTATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCG200.1289573795860468No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGAAATGCCACCTCGTATGCC170.10961377264813979No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCGTA309.592077E-870.0000138
TCGGCAT150.002183612270.0000136
TGCCATA309.592077E-870.0000133
CCATATC309.592077E-870.0000135
GCCATAT309.592077E-870.0000134
ATATCGT309.592077E-870.0000137
CATATCG309.592077E-870.0000136
ATCGTAT401.200533E-1070.039
GAAAAAA7450.061.5436260
TGAAAAA7400.061.4864959
CTTGAAA7250.061.31034557
TTCTGCT7200.061.24999652
TTGAAAA7200.061.24999658
TCTCGTA5850.061.02564238
CCATCTC5800.060.94827735
TGCCATC5800.060.94827733
GTCTTCT7300.060.89041549
GCTTGAA7300.060.89041556
GCCATCT5750.060.86956434
GCCGTCT7550.060.72847746
Produced by FastQC (version 0.11.5)