##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1781208_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	15509
Sequences flagged as poor quality	0
Sequence length	76
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.2707460184409	34.0	34.0	34.0	32.0	34.0
2	33.22296730930427	34.0	34.0	34.0	32.0	34.0
3	33.22690050938165	34.0	34.0	34.0	32.0	34.0
4	33.37107485975885	34.0	34.0	34.0	33.0	34.0
5	33.44058288735573	34.0	34.0	34.0	34.0	34.0
6	36.89992907344123	38.0	37.0	38.0	35.0	38.0
7	36.63040815010639	38.0	37.0	38.0	34.0	38.0
8	36.481011025855956	38.0	37.0	38.0	34.0	38.0
9	36.570958798117225	38.0	37.0	38.0	34.0	38.0
10-11	36.36975304661809	38.0	37.0	38.0	34.0	38.0
12-13	36.21455284028629	38.0	37.0	38.0	33.5	38.0
14-15	36.20839512541105	38.0	37.0	38.0	33.0	38.0
16-17	35.89164356180282	38.0	36.0	38.0	31.0	38.0
18-19	35.70655748275195	38.0	36.0	38.0	31.0	38.0
20-21	35.73147849635696	38.0	36.0	38.0	31.0	38.0
22-23	36.09833000193436	38.0	36.0	38.0	32.5	38.0
24-25	35.8262299310078	38.0	36.0	38.0	31.0	38.0
26-27	35.50896253788123	38.0	35.0	38.0	31.0	38.0
28-29	35.71984009284931	38.0	36.0	38.0	31.0	38.0
30-31	35.3207492423754	38.0	35.0	38.0	31.0	38.0
32-33	35.65903668837449	38.0	35.5	38.0	31.5	38.0
34-35	35.30350119285576	38.0	36.0	38.0	29.0	38.0
36-37	35.10323038235863	37.5	35.5	38.0	27.0	38.0
38-39	31.71219936810884	36.0	29.0	38.0	11.0	38.0
40-41	34.13527629118576	37.0	34.0	38.0	25.0	38.0
42-43	35.35598684634728	37.0	35.0	38.0	30.5	38.0
44-45	35.36062931201238	38.0	35.5	38.0	31.0	38.0
46-47	35.098426719969055	38.0	35.5	38.0	27.0	38.0
48-49	35.032593977690375	38.0	35.0	38.0	27.0	38.0
50-51	34.45573537945709	37.0	34.0	38.0	25.0	38.0
52-53	34.38142368947063	37.0	34.0	38.0	25.0	38.0
54-55	34.48597588497002	37.0	34.0	38.0	26.0	38.0
56-57	34.5258881939519	37.0	34.0	38.0	26.5	38.0
58-59	33.91833773937714	37.0	34.0	38.0	24.0	38.0
60-61	33.76968212005932	37.0	34.0	38.0	23.5	38.0
62-63	33.947546585853374	37.0	34.0	38.0	23.5	38.0
64-65	33.911212844155	37.0	34.0	38.0	23.5	38.0
66-67	33.14320717003031	37.0	32.0	38.0	21.5	38.0
68-69	31.8151718357083	36.5	29.5	38.0	11.0	38.0
70-71	28.860983944806243	33.5	18.0	38.0	10.5	38.0
72-73	26.44493519891676	30.0	11.0	38.0	11.0	38.0
74-75	26.51395963634019	30.5	11.0	38.0	11.0	38.0
76	25.874073118834225	27.0	11.0	37.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
21	2.0
22	6.0
23	22.0
24	53.0
25	88.0
26	196.0
27	281.0
28	448.0
29	580.0
30	819.0
31	989.0
32	1258.0
33	1592.0
34	1959.0
35	2409.0
36	1576.0
37	3231.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	23.45089947772261	8.195241472693276	9.665355599974209	58.68850344960991
2	12.992455993294216	8.865819846540719	58.79811722225804	19.34360693790702
3	12.934425172480497	10.11025855954607	57.95344638596944	19.001869882003998
4	10.129602166483977	48.28164291701593	20.916886968856794	20.671867947643303
5	11.2902185827584	19.19530595138307	58.02437294474176	11.490102521116771
6	19.64020891095493	57.18614997743246	11.702882197433748	11.470758914178864
7	17.3576632922819	17.77032690695725	10.980720871751885	53.891288929008965
8	15.120252756463989	58.70784705654781	9.014120833064672	17.157779353923527
9	12.99890386227352	12.379908440260493	8.994777226126766	65.62641047133923
10-11	16.345347862531433	34.199497066219614	31.484944225933333	17.970210845315624
12-13	15.513572764201431	10.02643626281514	34.7153265845638	39.74466438841963
14-15	14.981623573408989	9.42356051325037	12.979560255335612	62.61525565800503
16-17	38.62273518602102	31.06905667676833	9.510606744470952	20.7976013927397
18-19	39.1256689664066	13.601779611838289	9.92004642465665	37.35250499709846
20-21	16.60971049068283	10.477787091366302	9.826552324456767	63.085950093494105
22-23	15.884325230511315	33.33225868850345	9.571861499774323	41.211554581210905
24-25	38.7033335482623	32.155522599780774	9.871687407311883	19.269456444645044
26-27	39.88974144045393	34.9861370816945	9.678251337932814	15.445870139918757
28-29	37.404087948932876	14.755948159133405	10.065123476690955	37.774840415242764
30-31	19.11793152363144	53.91063253594687	10.126378231994327	16.845057708427362
32-33	15.065445870139918	37.871558449932294	31.091624218195886	15.971371461731898
34-35	38.0101876329873	11.577148752337353	14.375523889354568	36.037139725320785
36-37	16.290540976207364	30.695080275968795	10.851763492165839	42.162615255658004
38-39	15.042878328712362	14.73338061770585	31.052937004320075	39.17080404926172
40-41	14.884905538719453	10.290798890966535	35.443935779224965	39.38035979108904
42-43	38.00051582951834	9.549293958346766	33.25488426075182	19.19530595138307
44-45	15.245986201560385	32.326391127732286	36.13063382552066	16.296988845186668
46-47	36.86569088916114	11.209620220517118	15.70378489909085	36.2209039912309
48-49	36.25959120510671	9.542846089367464	11.999484170481656	42.19807853504417
50-51	16.774131149655037	10.007092655877232	34.78947707782578	38.42929911664195
52-53	13.447030756335032	9.626668386098395	57.03140112192921	19.894899735637374
54-55	13.52440518408666	9.952285769553162	37.85543877748404	38.667870268876136
56-57	36.41756399509962	10.071571345670256	16.126120317235156	37.38474434199497
58-59	14.588303565671545	11.038751692565608	54.594106647752916	19.778838094009927
60-61	36.394996453672064	31.84602488877426	15.929460313366434	15.829518344187246
62-63	14.469017989554454	58.31130311432071	10.809852343800374	16.409826552324457
64-65	13.008575665742473	59.87813527629119	10.597072667483397	16.516216390482946
66-67	13.218131407569796	59.587981172222584	10.44232381198014	16.75156360822748
68-69	13.733960925913985	58.20491327616223	11.090334644400025	16.97079115352376
70-71	14.65923012444387	48.12689406151267	15.071893739119222	22.141982074924236
72-73	17.88638854858469	33.65142820297892	20.19794957766458	28.26423367077181
74-75	18.476964245413804	29.99967759615695	22.52635651416965	28.9970016442596
76	19.16951447546586	29.84718550519053	21.600361080662843	29.382938938680763
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	1.5
16	5.5
17	8.0
18	6.0
19	4.0
20	6.5
21	9.0
22	12.0
23	21.0
24	25.0
25	23.0
26	28.5
27	31.5
28	36.0
29	43.0
30	50.5
31	63.5
32	73.0
33	77.0
34	85.0
35	114.0
36	122.5
37	110.0
38	124.5
39	156.5
40	527.5
41	1314.0
42	1747.0
43	1988.5
44	2047.5
45	1508.5
46	1152.0
47	877.5
48	470.5
49	275.0
50	212.0
51	183.0
52	138.5
53	131.5
54	140.0
55	146.5
56	152.5
57	154.5
58	157.0
59	181.0
60	207.5
61	226.5
62	243.0
63	257.5
64	261.0
65	276.0
66	304.0
67	306.0
68	307.5
69	315.5
70	334.0
71	346.0
72	329.0
73	321.0
74	296.0
75	262.0
76	248.0
77	206.5
78	172.0
79	165.0
80	135.5
81	87.0
82	65.5
83	63.0
84	49.5
85	25.0
86	13.0
87	12.0
88	6.0
89	1.0
90	1.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0032239344896511702
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	15509.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.27338964472242
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.39225104066603	30.36301502353472
2	17.034902337495996	13.72106518795538
3	4.755043227665706	5.745051260558386
4	1.392891450528338	2.2438584047972143
5	0.48030739673390976	0.9671803468953512
6	0.19212295869356388	0.46424656650976853
7	0.09606147934678194	0.2708104971306983
8	0.09606147934678194	0.30949771100651235
9	0.04803073967339097	0.1740924624411632
>10	0.3682356708293308	3.14011219292024
>50	0.04803073967339097	1.6893416725772132
>100	0.08005123278898495	8.640144432265135
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.016010246557796988	32.27158424140821
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCGT	5005	32.27158424140821	No Hit
TCTTATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCG	450	2.901541040686053	No Hit
CTTATACACATCTCCGAGCCCACGAGACGAAATGCCACCTCGTATGCCGT	340	2.1922754529627957	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCC	229	1.4765619962602359	No Hit
CTTATACACATCTCCGAGCCCACGAGACGAAATGCCAACTCGTATGCCGT	215	1.3862918305500032	No Hit
CTTATACACATCTCCGAGCCCACGAGACGAAATGCCAGCTCGTATGCCGT	106	0.6834741118060481	No Hit
ATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCGTCTT	98	0.6318911599716294	RNA PCR Primer, Index 20 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACGAAATGCCATATCGTATGCCGT	84	0.5416209942613966	No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCC	80	0.5158295183441872	No Hit
TCTTATACACATCTCCGAGCCCACGAGACGAAATGCCACCTCGTATGCCG	48	0.30949771100651235	No Hit
CTTATACACATCTCCGAGCCCACGAGACGAAATGCCATTTCGTATGCCGT	42	0.2708104971306983	No Hit
TCTTATACACATCTCCGAGCCCACGAGACGAAATGCCAACTCGTATGCCG	33	0.21277967631697725	No Hit
CTTATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCGC	32	0.2063318073376749	No Hit
CTATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCGTC	30	0.1934360693790702	No Hit
CTTATACACATCTCCGAGCCCACGAGACGAAATGCCATCCCGTATGCCGT	29	0.18698820039976788	No Hit
CTTTACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCGTC	24	0.15474885550325618	No Hit
TCTTAATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCC	23	0.14830098652395385	No Hit
CTTTATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCG	22	0.1418531175446515	No Hit
CTTATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCGA	21	0.13540524856534916	No Hit
TATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCGTCT	20	0.1289573795860468	No Hit
CCTTATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCG	20	0.1289573795860468	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGAAATGCCACCTCGTATGCC	17	0.10961377264813979	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0064478689793023404	0.0
2	0.0	0.0	0.0	0.0064478689793023404	0.0
3	0.0	0.0	0.0	0.0064478689793023404	0.0
4	0.0	0.0	0.0	0.0064478689793023404	0.0
5	0.0	0.0	0.0	0.0064478689793023404	0.0
6	0.0	0.0	0.0	0.0064478689793023404	0.0
7	0.0	0.0	0.0	0.012895737958604681	0.0
8	0.0	0.0	0.0	0.012895737958604681	0.0
9	0.0	0.0	0.0	0.012895737958604681	0.0
10	0.0	0.0	0.0	0.019343606937907022	0.0
11	0.0	0.0	0.0	0.019343606937907022	0.0
12	0.0	0.0	0.0	0.019343606937907022	0.0
13	0.0	0.0	0.0	0.019343606937907022	0.0
14	0.0	0.0	0.0	0.019343606937907022	0.0
15	0.0	0.0	0.0	0.019343606937907022	0.0
16	0.0	0.0	0.0	0.019343606937907022	0.0
17	0.0	0.0	0.0	0.019343606937907022	0.0
18	0.0	0.0	0.0	0.0322393448965117	0.0
19	0.0	0.0	0.0	0.0322393448965117	0.0
20	0.0	0.0	0.0	0.0322393448965117	0.0
21	0.0	0.0	0.0	0.038687213875814044	0.0
22	0.0	0.0	0.0	0.038687213875814044	0.0
23	0.0	0.0	0.0	0.038687213875814044	0.0
24	0.0	0.0	0.0	0.04513508285511639	0.0
25	0.0	0.0	0.0	0.04513508285511639	0.0
26	0.0	0.0	0.0	0.04513508285511639	0.0
27	0.0	0.0	0.0	0.09027016571023277	0.0
28	0.0	0.0	0.0	0.12250951060674448	0.0
29	0.0	0.0	0.0	0.1418531175446515	0.0
30	0.0	0.0	0.0	0.1934360693790702	0.0
31	0.0	0.0	0.0	0.21922754529627958	0.0
32	0.0	0.0	0.0	0.2901541040686053	0.0
33	0.0	0.0	0.0	0.36752853182023343	0.0
34	0.0	0.0	0.0	0.4835901734476755	0.0
35	0.0	0.0	0.0	0.60609968405442	0.0
36	0.0	0.0	0.0	0.8188793603713973	0.0
37	0.0	0.0	0.0	1.250886581984654	0.0
38	0.0	0.0	0.0	1.605519375846283	0.0
39	0.0	0.0	0.0	1.9666000386872138	0.0
40	0.0	0.0	0.0	2.3212328325488425	0.0
41	0.0	0.0	0.0	2.682313495389774	0.0
42	0.0	0.0	0.0	2.972467599458379	0.0
43	0.0	0.0	0.0	3.3593397382165193	0.0
44	0.0	0.0	0.0	3.7720033528918693	0.0
45	0.0	0.0	0.0	4.249145657360242	0.0
46	0.0	0.0	0.0	4.823005996518151	0.0
47	0.0	0.0	0.0	5.300148300986524	0.0
48	0.0	0.0	0.0	5.654781094848152	0.0
49	0.0	0.0	0.0	6.009413888709782	0.0
50	0.0	0.0	0.0	6.383390289509317	0.0
51	0.0	0.0	0.0	6.873428331936295	0.0
52	0.0	0.0	0.0	7.260300470694435	0.0
53	0.0	0.0	0.0	7.621381133535366	0.0
54	0.0	0.0	0.0	7.995357534334902	0.0
55	0.0	0.0	0.0	8.37578180411374	0.0
56	0.0	0.0	0.0	8.672383777161649	0.0
57	0.0	0.0	0.0	8.956090012250952	0.0
58	0.0	0.0	0.0	9.278483461216068	0.0
59	0.0	0.0	0.0	9.652459862015604	0.0
60	0.0	0.0	0.0	9.858791669353279	0.0
61	0.0	0.0	0.0	10.13605003546328	0.0
62	0.0	0.0	0.0	10.38751692565607	0.0
63	0.0	0.0	0.0	10.71635824360049	0.0
64	0.0	0.0	0.0	11.096782513379328	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATCGTA	30	9.592077E-8	70.00001	38
TCGGCAT	15	0.0021836122	70.00001	36
TGCCATA	30	9.592077E-8	70.00001	33
CCATATC	30	9.592077E-8	70.00001	35
GCCATAT	30	9.592077E-8	70.00001	34
ATATCGT	30	9.592077E-8	70.00001	37
CATATCG	30	9.592077E-8	70.00001	36
ATCGTAT	40	1.200533E-10	70.0	39
GAAAAAA	745	0.0	61.54362	60
TGAAAAA	740	0.0	61.48649	59
CTTGAAA	725	0.0	61.310345	57
TTCTGCT	720	0.0	61.249996	52
TTGAAAA	720	0.0	61.249996	58
TCTCGTA	585	0.0	61.025642	38
CCATCTC	580	0.0	60.948277	35
TGCCATC	580	0.0	60.948277	33
GTCTTCT	730	0.0	60.890415	49
GCTTGAA	730	0.0	60.890415	56
GCCATCT	575	0.0	60.869564	34
GCCGTCT	755	0.0	60.728477	46
>>END_MODULE