FastQCFastQC Report
Wed 25 May 2016
SRR1781207_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781207_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences179241
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGAAGC150.002221832570.066
CTCGTAA200.00694251252.50000430
CAACCGG200.00694251252.50000418
GTGCTAT200.00694251252.50000427
CCTGGTA200.00694251252.5000042
TAATCGA200.00694251252.50000421
GCAACCG305.8660546E-446.66666417
GGGTAAT404.5792196E-543.7500041
AGTGCTA350.001252934940.026
CTTGTAC501.704739E-435.0000043
CCGTGCG501.704739E-435.0000049
TAACAAG400.002414005835.00000431
AGTAAAT400.002414005835.0000042
TTGTACT552.9834532E-431.8181844
GGCATAA552.9834532E-431.81818421
TCTGCCG450.00429885631.11111364
GAGTTGG450.00429885631.1111137
CGAAGTT450.00429885631.11111333
GCTCGGA604.9677206E-429.16666611
TGCCAAA500.00719439228.00000215
Produced by FastQC (version 0.11.5)