##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1781207_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	179241
Sequences flagged as poor quality	0
Sequence length	76
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.127855791922606	34.0	32.0	34.0	21.0	34.0
2	31.19984266992485	34.0	32.0	34.0	23.0	34.0
3	31.147628053849285	34.0	33.0	34.0	23.0	34.0
4	31.307569138757316	34.0	33.0	34.0	23.0	34.0
5	31.256565183189114	34.0	33.0	34.0	23.0	34.0
6	33.98633125233624	37.0	34.0	38.0	23.0	38.0
7	33.92967568804013	37.0	34.0	38.0	24.0	38.0
8	33.85801239671727	37.0	34.0	38.0	23.0	38.0
9	34.11038211123571	37.0	34.0	38.0	24.0	38.0
10-11	33.94757616839897	37.0	34.0	38.0	23.0	38.0
12-13	33.91676848488906	37.0	34.0	38.0	23.0	38.0
14-15	33.78368230482981	37.0	34.0	38.0	22.5	38.0
16-17	33.76740254740824	37.0	34.0	38.0	22.5	38.0
18-19	33.80007085432462	37.0	34.0	38.0	23.0	38.0
20-21	33.72791381436167	37.0	34.0	38.0	22.5	38.0
22-23	33.77686187869963	37.0	34.0	38.0	22.5	38.0
24-25	33.7780948555297	37.0	34.0	38.0	22.5	38.0
26-27	33.679057804854914	37.0	34.0	38.0	22.0	38.0
28-29	33.76562561021195	37.0	34.0	38.0	22.5	38.0
30-31	33.832513208473515	37.0	34.0	38.0	23.0	38.0
32-33	33.78577167054413	37.0	34.0	38.0	22.5	38.0
34-35	33.813326750018135	37.0	34.0	38.0	23.0	38.0
36-37	33.65674706121925	37.0	34.0	38.0	22.0	38.0
38-39	33.68068968595355	37.0	34.0	38.0	22.0	38.0
40-41	33.67761561249937	37.0	34.0	38.0	22.0	38.0
42-43	33.759485831924614	37.0	34.0	38.0	22.5	38.0
44-45	33.78326387377888	37.0	34.0	38.0	22.5	38.0
46-47	33.792725994610606	37.0	34.0	38.0	22.5	38.0
48-49	33.761656094308776	37.0	34.0	38.0	22.5	38.0
50-51	33.62694361223158	37.0	34.0	38.0	22.0	38.0
52-53	33.7038958720382	37.0	34.0	38.0	22.5	38.0
54-55	33.66817580799036	37.0	34.0	38.0	22.0	38.0
56-57	33.641404589351765	37.0	34.0	38.0	22.0	38.0
58-59	33.637647078514405	37.0	34.0	38.0	22.0	38.0
60-61	33.61519685786176	37.0	34.0	38.0	22.0	38.0
62-63	33.55891788151149	37.0	34.0	38.0	22.0	38.0
64-65	33.58717592515106	37.0	34.0	38.0	22.0	38.0
66-67	33.47875765031438	37.0	34.0	38.0	22.0	38.0
68-69	33.2305052973371	37.0	33.5	38.0	21.0	38.0
70-71	33.37989355114064	37.0	34.0	38.0	21.5	38.0
72-73	33.31806338951468	37.0	33.5	38.0	21.0	38.0
74-75	33.29739010605833	37.0	33.0	38.0	21.0	38.0
76	31.847852890800652	36.0	30.0	38.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	2.0
13	27.0
14	115.0
15	392.0
16	857.0
17	1496.0
18	1981.0
19	1996.0
20	1868.0
21	1705.0
22	1701.0
23	1874.0
24	2167.0
25	2474.0
26	2941.0
27	3578.0
28	4187.0
29	4990.0
30	5910.0
31	7094.0
32	8600.0
33	10500.0
34	13341.0
35	17480.0
36	26310.0
37	55655.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.95679002014048	18.56439095965767	10.172337802176958	26.306481218024896
2	23.294335559386525	19.92903409376203	31.06320540501336	25.71342494183809
3	19.930149909897846	21.533577697067077	28.42485815187373	30.111414241161345
4	14.542431698104785	15.503149391043344	36.06261960154205	33.89179930930981
5	14.50616767369073	33.2658264571164	34.912213165514586	17.315792703678287
6	31.223325020503122	35.09688073599232	17.992535190051385	15.687259053453174
7	30.191753002940175	27.2900731417477	22.20027783821782	20.317896017094302
8	26.827009445383588	32.3547625822217	19.576994102911723	21.241233869482986
9	25.108652596225195	15.708459560033697	21.31431982637901	37.8685680173621
10-11	26.40913630252007	23.69184505777138	26.601335631914573	23.297683007793974
12-13	26.00883726379567	22.022863072622894	25.98540512494351	25.982894538637925
14-15	23.94959858514514	23.304656858642833	23.407590897171964	29.33815365904006
16-17	24.344597497224406	26.129345406463923	24.876283885941277	24.649773210370395
18-19	23.77720499216139	25.412991447269317	25.996284332267727	24.813519228301562
20-21	23.691008195669518	25.751920598523775	25.886655396923697	24.67041580888301
22-23	24.17359867441043	25.311731132943915	26.094197198185682	24.420472994459974
24-25	23.74261469195106	25.467945391958313	25.836164716777972	24.953275199312657
26-27	23.102415184025975	25.74438883960701	26.23451107726469	24.918684899102324
28-29	23.815979602881036	25.268493257681	27.045430453969793	23.870096685468166
30-31	23.759630888022272	25.346321433154245	26.10172895710245	24.792318721721035
32-33	23.370768964689997	25.881634224312517	25.873544557327843	24.87405225366964
34-35	24.18140938736115	24.91561640472883	26.41276270496148	24.490211502948544
36-37	24.322002220474108	24.66232614189834	25.96085716995554	25.054814467672017
38-39	23.44636550789161	25.39625420523206	26.982665796330078	24.174714490546247
40-41	23.658091619662912	24.387277464419412	27.2998365329361	24.654794382981574
42-43	23.203396544317428	24.27123258629443	27.090342053436434	25.43502881595171
44-45	22.54478607015136	24.438883960700956	27.69567230711723	25.32065766203045
46-47	22.80923449434002	24.05560111804777	27.81255404734408	25.32261034026813
48-49	21.996920347465146	23.863401788653267	28.734218175529037	25.405459688352554
50-51	21.638743367867843	24.22241563035243	28.34340357395908	25.795437427820644
52-53	21.551709709274107	24.04862726719891	28.804793546119473	25.59486947740751
54-55	20.700063043611674	24.854525471292842	28.62905250472827	25.816358980367216
56-57	20.67718881282742	25.06262518062274	28.579677640718366	25.68050836583148
58-59	20.915694511858334	24.79957152660385	28.064728494038754	26.220005467499064
60-61	20.47801563258406	24.355755658582577	28.412584174379745	26.753644534453613
62-63	20.629208718987286	24.055880072081724	28.456101003676615	26.85881020525438
64-65	21.448217762677064	24.214883871435664	27.530252564982344	26.806645800904928
66-67	21.44710194654125	23.93592983748138	26.891168873193077	27.725799342784295
68-69	21.435943785183078	24.295501587248452	26.53578143393532	27.732773193633154
70-71	21.884780825815522	24.159372018678763	26.408020486384252	27.547826669121463
72-73	21.950892931862686	23.707745437706777	26.36422470305343	27.977136927377106
74-75	22.43934144531664	24.067596141507803	26.090849749778233	27.402212663397325
76	23.063919527340285	23.919192595444123	25.354690054172874	27.662197823042717
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.5
8	1.0
9	0.5
10	0.0
11	0.5
12	1.0
13	1.0
14	3.0
15	6.5
16	9.0
17	10.0
18	25.0
19	42.5
20	79.5
21	114.0
22	142.5
23	203.0
24	298.0
25	361.0
26	452.0
27	644.0
28	905.0
29	1065.0
30	1227.5
31	1582.5
32	2188.5
33	2602.0
34	2954.5
35	3738.5
36	4769.0
37	5368.0
38	5917.5
39	7189.0
40	8766.0
41	9819.5
42	10018.0
43	10375.0
44	10949.0
45	11088.0
46	11010.0
47	10902.0
48	10217.5
49	9159.5
50	8678.0
51	8150.0
52	6882.0
53	5602.5
54	5063.0
55	4711.0
56	3970.0
57	3263.5
58	2946.0
59	2788.5
60	2482.5
61	2293.0
62	2252.0
63	2220.0
64	2123.5
65	2075.0
66	2031.5
67	1972.0
68	1940.0
69	1890.0
70	1847.0
71	1822.0
72	1820.0
73	1748.0
74	1578.0
75	1478.0
76	1411.0
77	1240.0
78	1015.0
79	894.0
80	798.5
81	601.0
82	417.5
83	336.0
84	276.5
85	170.0
86	102.0
87	81.0
88	59.0
89	33.0
90	22.5
91	8.5
92	1.0
93	1.5
94	3.0
95	3.0
96	2.0
97	1.5
98	0.5
99	2.5
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	179241.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.45619026896748
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.97068802314327	37.753081047305024
2	13.620071684587815	14.289141435274297
3	5.6167108048030805	8.838937519875476
4	2.541931229592759	5.333601129205929
5	1.4166746434383077	3.7156677322710765
6	0.9412590536358125	2.962491840594507
7	0.6732395264988353	2.4720906489028738
8	0.5009412590536358	2.102197599879492
9	0.40415642980972744	1.9080455922473094
>10	2.290928815289876	19.874917011174897
>50	0.023398530146878954	0.7498284432691181
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	5.5790806790857E-4	0.0
10	0.0	0.0	0.0	5.5790806790857E-4	0.0
11	0.0	0.0	0.0	5.5790806790857E-4	0.0
12	0.0	0.0	0.0	5.5790806790857E-4	0.0
13	0.0	0.0	0.0	5.5790806790857E-4	0.0
14	0.0	0.0	0.0	5.5790806790857E-4	0.0
15	0.0	0.0	0.0	5.5790806790857E-4	0.0
16	0.0	0.0	0.0	5.5790806790857E-4	0.0
17	0.0	0.0	0.0	5.5790806790857E-4	0.0
18	0.0	0.0	0.0	5.5790806790857E-4	0.0
19	0.0	0.0	0.0	0.00278954033954285	0.0
20	0.0	0.0	0.0	0.00334744840745142	0.0
21	0.0	0.0	0.0	0.00390535647535999	0.0
22	0.0	0.0	0.0	0.0055790806790857	0.0
23	0.0	0.0	0.0	0.0055790806790857	0.0
24	0.0	0.0	0.0	0.007252804882811411	0.0
25	0.0	0.0	0.0	0.00892652908653712	0.0
26	0.0	0.0	0.0	0.01060025329026283	0.0
27	0.0	0.0	0.0	0.024547954987977082	0.0
28	0.0	0.0	0.0	0.04240101316105132	0.0
29	0.0	0.0	0.0	0.07141223269229696	0.0
30	0.0	0.0	0.0	0.08982319893327978	0.0
31	0.0	0.0	0.0	0.11046579744589687	0.0
32	0.0	0.0	0.0	0.14617191379204536	0.0
33	0.0	0.0	0.0	0.20084690444708522	0.0
34	0.0	0.0	0.0	0.2901121953124564	0.0
35	0.0	0.0	0.0	0.4184310509314275	0.0
36	0.0	0.0	0.0	0.6187200473106041	0.0
37	0.0	0.0	0.0	0.9607176929385576	0.0
38	0.0	0.0	0.0	1.3490217082029223	0.0
39	0.0	0.0	0.0	1.8405387160303726	0.0
40	0.0	0.0	0.0	2.3973309678031254	0.0
41	0.0	0.0	0.0	3.0472938669166094	0.0
42	0.0	0.0	0.0	3.7787113439447446	0.0
43	0.0	0.0	0.0	4.63119487170904	0.0
44	0.0	0.0	0.0	5.564575069320077	0.0
45	0.0	0.0	0.0	6.651379985605971	0.0
46	0.0	0.0	0.0	7.700247153274083	0.0
47	0.0	0.0	0.0	8.76696737911527	0.0
48	0.0	0.0	0.0	9.775107257826056	0.0
49	0.0	0.0	0.0	10.792731573691286	0.0
50	0.0	0.0	0.0	11.685942390412908	0.0
51	0.0	0.0	0.0	12.591427184628516	0.0
52	0.0	0.0	0.0	13.431078826830914	0.0
53	0.0	0.0	0.0	14.336005712978615	0.0
54	0.0	0.0	0.0	15.36869354667738	0.0
55	0.0	0.0	0.0	16.42090816275294	0.0
56	0.0	0.0	0.0	17.39334192511758	0.0
57	0.0	0.0	0.0	18.284321109567564	0.0
58	0.0	0.0	0.0	19.152983971301207	0.0
59	0.0	0.0	0.0	19.960834853632818	0.0
60	0.0	0.0	0.0	20.78821251834123	0.0
61	0.0	0.0	0.0	21.557567743987146	0.0
62	0.0	0.0	0.0	22.356492097232216	0.0
63	0.0	0.0	0.0	23.208417716928604	0.0
64	0.0	0.0	0.0	23.996741816883414	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGAAGC	15	0.0022218325	70.0	66
CTCGTAA	20	0.006942512	52.500004	30
CAACCGG	20	0.006942512	52.500004	18
GTGCTAT	20	0.006942512	52.500004	27
CCTGGTA	20	0.006942512	52.500004	2
TAATCGA	20	0.006942512	52.500004	21
GCAACCG	30	5.8660546E-4	46.666664	17
GGGTAAT	40	4.5792196E-5	43.750004	1
AGTGCTA	35	0.0012529349	40.0	26
CTTGTAC	50	1.704739E-4	35.000004	3
CCGTGCG	50	1.704739E-4	35.000004	9
TAACAAG	40	0.0024140058	35.000004	31
AGTAAAT	40	0.0024140058	35.000004	2
TTGTACT	55	2.9834532E-4	31.818184	4
GGCATAA	55	2.9834532E-4	31.818184	21
TCTGCCG	45	0.004298856	31.111113	64
GAGTTGG	45	0.004298856	31.111113	7
CGAAGTT	45	0.004298856	31.111113	33
GCTCGGA	60	4.9677206E-4	29.166666	11
TGCCAAA	50	0.007194392	28.000002	15
>>END_MODULE