FastQCFastQC Report
Wed 25 May 2016
SRR1781207_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781207_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences162042
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCGT50283.102899248343022No Hit
TCTTATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCG5800.3579318942002691No Hit
CTTATACACATCTCCGAGCCCACGAGACCAAGAATTACCTCGTATGCCGT3220.1987139136767011No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCC3000.1851371866553116No Hit
CTTATACACATCTCCGAGCCCACGAGACCAAGAATTAACTCGTATGCCGT1810.11169943594870466No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCATG150.002221446770.040
CTCGAAA150.002221446770.057
TACCTCG650.059.23077436
CGTATGC7700.053.63636441
CGTCTTC7550.053.31125648
CTCGTAT7750.053.29032539
TCGTATG7750.053.29032540
CTTGAAA7300.053.2191857
CCGTCTT7600.052.9605347
TATGCCG7750.052.8387143
TTGGACG200.00694131652.549
CGCATGC200.00694131652.541
TCGTAAC200.00694131652.517
CGTAACA200.00694131652.518
GTAAGTG200.00694131652.527
AGTGTAC200.00694131652.530
CTGCTTG7600.052.0394854
GCCGTCT7750.051.93548646
GTATGCC8000.051.18750442
TCTCGTA6850.051.09488738