##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1781207_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	162042
Sequences flagged as poor quality	0
Sequence length	76
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.25977215783562	34.0	34.0	34.0	32.0	34.0
2	33.29220202169808	34.0	34.0	34.0	33.0	34.0
3	33.31688080867923	34.0	34.0	34.0	33.0	34.0
4	33.37539650214142	34.0	34.0	34.0	33.0	34.0
5	33.38567161600079	34.0	34.0	34.0	33.0	34.0
6	36.81073425408228	38.0	37.0	38.0	34.0	38.0
7	36.68751928512361	38.0	37.0	38.0	34.0	38.0
8	36.48327594080547	38.0	37.0	38.0	34.0	38.0
9	36.44585354414288	38.0	37.0	38.0	34.0	38.0
10-11	36.36288739956308	38.0	37.0	38.0	34.0	38.0
12-13	36.29361832117599	38.0	37.0	38.0	34.0	38.0
14-15	36.325656311326696	38.0	37.0	38.0	34.0	38.0
16-17	36.222908258352774	38.0	37.0	38.0	34.0	38.0
18-19	36.209618493970694	38.0	37.0	38.0	33.5	38.0
20-21	36.16534910702163	38.0	37.0	38.0	33.0	38.0
22-23	36.24749756236037	38.0	37.0	38.0	34.0	38.0
24-25	36.241141185618545	38.0	37.0	38.0	34.0	38.0
26-27	35.992214981301146	38.0	36.5	38.0	32.0	38.0
28-29	35.975657545574606	38.0	37.0	38.0	32.0	38.0
30-31	36.027844632872956	38.0	37.0	38.0	32.0	38.0
32-33	35.95257710963824	38.0	37.0	38.0	32.0	38.0
34-35	35.92926833783834	38.0	36.5	38.0	31.5	38.0
36-37	35.865565717530025	38.0	36.0	38.0	31.0	38.0
38-39	35.609110600955304	38.0	36.0	38.0	31.0	38.0
40-41	35.795003764456126	38.0	36.0	38.0	31.0	38.0
42-43	35.867771935671	38.0	36.0	38.0	31.5	38.0
44-45	35.844102146357116	38.0	36.0	38.0	31.0	38.0
46-47	35.83847706150257	38.0	36.0	38.0	31.0	38.0
48-49	35.80385949321781	38.0	36.0	38.0	31.0	38.0
50-51	35.70947346984116	38.0	36.0	38.0	31.0	38.0
52-53	35.743504770368176	38.0	36.0	38.0	31.0	38.0
54-55	35.754822823712374	38.0	36.0	38.0	31.0	38.0
56-57	35.72812604139668	38.0	36.0	38.0	31.0	38.0
58-59	35.66965971785093	38.0	36.0	38.0	31.0	38.0
60-61	35.66693511558732	38.0	36.0	38.0	31.0	38.0
62-63	35.63869552338282	38.0	36.0	38.0	31.0	38.0
64-65	35.594176201231775	38.0	36.0	38.0	31.0	38.0
66-67	35.525388479529994	38.0	36.0	38.0	31.0	38.0
68-69	35.382388516557434	38.0	36.0	38.0	31.0	38.0
70-71	35.09126954740129	38.0	36.0	38.0	28.0	38.0
72-73	34.861807432640916	38.0	36.0	38.0	27.0	38.0
74-75	34.889877315757644	38.0	36.0	38.0	27.0	38.0
76	33.94711864825169	37.0	34.0	38.0	24.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	1.0
20	2.0
21	12.0
22	29.0
23	50.0
24	123.0
25	292.0
26	562.0
27	980.0
28	1643.0
29	2470.0
30	3853.0
31	5447.0
32	7506.0
33	10251.0
34	13691.0
35	18878.0
36	26959.0
37	69292.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.807679490502466	18.216882042927143	10.33559200701053	29.639846459559866
2	22.209056911171178	18.820429271423457	33.67830562446773	25.292208192937633
3	19.805359104429716	20.983448735513015	29.91940361140939	29.29178854864788
4	14.357388825119413	18.9648362770146	34.168919169104306	32.508855728761674
5	14.26420310783624	33.10191185001419	36.69727601486035	15.936609027289222
6	31.085150763382334	38.464719023463054	16.060033818392764	14.390096394761853
7	29.183791856432283	27.21022944668666	20.593426395625826	23.01255230125523
8	25.60015304674097	35.72715715678651	18.148381283864676	20.524308512607842
9	23.824687426716533	15.177546562002444	19.623307537551995	41.37445847372903
10-11	25.512830007035213	25.26937460658348	26.51380506288493	22.703990323496377
12-13	25.01882228064329	21.502449982103407	26.448698485577815	27.03002925167549
14-15	22.855802816553734	22.377840313005272	22.215228150726354	32.55112871971464
16-17	25.483516619148123	27.233988718974096	23.26187037928438	24.020624282593403
18-19	25.41655866997445	24.973772231890496	24.154540180940746	25.45512891719431
20-21	22.941274484392935	24.847878944964886	23.999024944150282	28.211821626491897
22-23	23.52167956455734	26.551449624171514	24.242480344602015	25.684390466669132
24-25	25.305784919959024	26.724552893694227	24.19033337036077	23.779328815985977
26-27	24.592698189358313	27.075079300428285	24.579430024314682	23.75279248589872
28-29	23.0585280359413	24.942607472136853	25.04258155293072	26.956282938991126
30-31	23.039397193320248	28.639797089643427	24.60689204033522	23.7139136767011
32-33	24.76919564063638	27.409560484318877	24.38904728403747	23.43219659100727
34-35	23.492983300625763	26.884696560151074	26.57582602041446	23.046494118808706
36-37	23.191209686377608	26.115142987620494	26.845817750953454	23.847829575048443
38-39	22.654620407054963	25.120647733303713	26.98405351698942	25.240678342651908
40-41	22.759222917515213	23.76359215512028	27.651781636859578	25.825403290504934
42-43	24.647005097443873	23.70681675121265	27.33180286592365	24.31437528541983
44-45	21.93628812283235	25.887424248034463	28.05970057145678	24.116587057676405
46-47	24.199590229693534	22.98539884721245	26.696165191740413	26.118845731353602
48-49	23.299514940570962	22.922760765727404	26.905987336616434	26.8717369570852
50-51	21.18709964083386	23.293652262993543	28.904543266560523	26.614704829612073
52-53	20.44716801816813	23.18442132286691	31.433208674294317	24.93520198467064
54-55	19.69952234605843	23.986991027017687	29.500067883635104	26.813418743288775
56-57	21.620320657607287	23.87868577282433	27.220720553930462	27.280273015637924
58-59	19.89576776391306	23.734895891188703	30.59638859061231	25.772947754285923
60-61	21.530837684057218	25.56435985732094	26.81033312351119	26.09446933511065
62-63	18.968539020747706	27.806371187716767	26.37649498278224	26.848594808753283
64-65	19.475197788227742	28.297293294331098	25.26628898680589	26.961219930635266
66-67	19.771725848854	28.21614149418052	24.42700040730181	27.58513224966367
68-69	19.51407659742536	28.297293294331098	24.06999419903482	28.118635909208724
70-71	20.037089149726615	27.565075721109345	23.941323854309378	28.45651127485467
72-73	20.05190012465904	26.01547746880438	24.280742029844117	29.651880376692464
74-75	20.472470100344356	26.645869589365724	23.710519494945757	29.17114081534417
76	20.753261500104912	27.219486306019427	23.292726577060268	28.734525616815393
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.5
11	1.5
12	2.0
13	2.5
14	2.0
15	10.5
16	18.0
17	16.0
18	18.5
19	33.5
20	61.5
21	77.0
22	104.5
23	163.5
24	272.0
25	349.0
26	429.0
27	541.5
28	726.5
29	879.0
30	985.5
31	1247.5
32	1815.5
33	2228.0
34	2500.0
35	3257.5
36	4092.5
37	4442.0
38	5201.5
39	7236.0
40	9324.0
41	10145.0
42	10153.0
43	10073.0
44	9925.5
45	9787.5
46	9717.0
47	9449.0
48	8748.0
49	7969.5
50	7624.0
51	7177.5
52	6083.5
53	4927.5
54	4419.0
55	4118.0
56	3446.0
57	2769.0
58	2463.0
59	2269.5
60	2010.5
61	1885.0
62	1825.0
63	1839.5
64	1832.0
65	1807.0
66	1857.5
67	1911.0
68	1875.0
69	1797.5
70	1726.5
71	1697.0
72	1686.5
73	1603.0
74	1423.5
75	1317.0
76	1306.0
77	1196.0
78	961.0
79	825.0
80	750.5
81	569.5
82	387.5
83	312.0
84	242.5
85	155.5
86	98.5
87	59.0
88	44.5
89	26.0
90	15.5
91	6.5
92	4.0
93	3.5
94	1.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	162042.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.65339850162304
#Duplication Level	Percentage of deduplicated	Percentage of total
1	59.38277823871044	24.734945261105146
2	19.82932321915373	16.519174041297934
3	8.319011496977598	10.395453030695744
4	3.7276283038994906	6.210735488330186
5	1.9941922484295365	4.153244220634156
6	1.26081545573071	3.1510349168734035
7	0.920054521749437	2.682637834635465
8	0.6770771601280077	2.25620518137273
9	0.5704041720990873	2.138334505868849
>10	3.2801943818892974	22.98293035139038
>50	0.028149816285409503	0.6905617062243122
>100	0.007407846390897238	0.6239123190284
>500	0.0014815692781794476	0.3579318942002691
>1k	0.0	0.0
>5k	0.0014815692781794476	3.102899248343022
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCGT	5028	3.102899248343022	No Hit
TCTTATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCG	580	0.3579318942002691	No Hit
CTTATACACATCTCCGAGCCCACGAGACCAAGAATTACCTCGTATGCCGT	322	0.1987139136767011	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCC	300	0.1851371866553116	No Hit
CTTATACACATCTCCGAGCCCACGAGACCAAGAATTAACTCGTATGCCGT	181	0.11169943594870466	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	6.171239555177052E-4	0.0
10	0.0	0.0	0.0	6.171239555177052E-4	0.0
11	0.0	0.0	0.0	6.171239555177052E-4	0.0
12	0.0	0.0	0.0	6.171239555177052E-4	0.0
13	0.0	0.0	0.0	6.171239555177052E-4	0.0
14	0.0	0.0	0.0	6.171239555177052E-4	0.0
15	0.0	0.0	0.0	6.171239555177052E-4	0.0
16	0.0	0.0	0.0	6.171239555177052E-4	0.0
17	0.0	0.0	0.0	6.171239555177052E-4	0.0
18	0.0	0.0	0.0	0.0012342479110354105	0.0
19	0.0	0.0	0.0	0.0030856197775885265	0.0
20	0.0	0.0	6.171239555177052E-4	0.0030856197775885265	0.0
21	0.0	0.0	6.171239555177052E-4	0.0030856197775885265	0.0
22	0.0	0.0	6.171239555177052E-4	0.004319867688623937	0.0
23	0.0	0.0	6.171239555177052E-4	0.006171239555177053	0.0
24	0.0	0.0	6.171239555177052E-4	0.006788363510694759	0.0
25	0.0	0.0	6.171239555177052E-4	0.008022611421730169	0.0
26	0.0	0.0	6.171239555177052E-4	0.009873983288283284	0.0
27	0.0	0.0	6.171239555177052E-4	0.0234507103096728	0.0
28	0.0	0.0	6.171239555177052E-4	0.04443292479727478	0.0
29	0.0	0.0	6.171239555177052E-4	0.07405487466212464	0.0
30	0.0	0.0	6.171239555177052E-4	0.09812270892731514	0.0
31	0.0	0.0	6.171239555177052E-4	0.1166364275928463	0.0
32	0.0	0.0	6.171239555177052E-4	0.15674948470149713	0.0
33	0.0	0.0	6.171239555177052E-4	0.2104392688315375	0.0
34	0.0	0.0	6.171239555177052E-4	0.30856197775885263	0.0
35	0.0	0.0	6.171239555177052E-4	0.4449463719282655	0.0
36	0.0	0.0	6.171239555177052E-4	0.6634082521815332	0.0
37	0.0	0.0	6.171239555177052E-4	1.030597005714568	0.0
38	0.0	0.0	6.171239555177052E-4	1.432344700756594	0.0
39	0.0	0.0	6.171239555177052E-4	1.9550486910800904	0.0
40	0.0	0.0	6.171239555177052E-4	2.5413164488219104	0.0
41	0.0	0.0	6.171239555177052E-4	3.220769923846904	0.0
42	0.0	0.0	6.171239555177052E-4	3.978598141222646	0.0
43	0.0	0.0	6.171239555177052E-4	4.887621727700226	0.0
44	0.0	0.0	6.171239555177052E-4	5.862060453462682	0.0
45	0.0	0.0	6.171239555177052E-4	6.990780168104566	0.0
46	0.0	0.0	6.171239555177052E-4	8.087409437059527	0.0
47	0.0	0.0	6.171239555177052E-4	9.216129151701411	0.0
48	0.0	0.0	6.171239555177052E-4	10.271411115636687	0.0
49	0.0	0.0	6.171239555177052E-4	11.339035558682317	0.0
50	0.0	0.0	6.171239555177052E-4	12.270892731514053	0.0
51	0.0	0.0	6.171239555177052E-4	13.216326631367176	0.0
52	0.0	0.0	6.171239555177052E-4	14.07351180558127	0.0
53	0.0	0.0	6.171239555177052E-4	15.008454598190593	0.0
54	0.0	0.0	6.171239555177052E-4	16.056948198615174	0.0
55	0.0	0.0	6.171239555177052E-4	17.12765826143839	0.0
56	0.0	0.0	6.171239555177052E-4	18.12863331728811	0.0
57	0.0	0.0	6.171239555177052E-4	19.084558324385036	0.0
58	0.0	0.0	6.171239555177052E-4	19.975068192197085	0.0
59	0.0	0.0	6.171239555177052E-4	20.86125819232051	0.0
60	0.0	0.0	6.171239555177052E-4	21.714740622801497	0.0
61	0.0	0.0	6.171239555177052E-4	22.520087384752102	0.0
62	0.0	0.0	6.171239555177052E-4	23.339627997679614	0.0
63	0.0	0.0	6.171239555177052E-4	24.217795386381308	0.0
64	0.0	0.0	6.171239555177052E-4	25.04659285864159	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCATG	15	0.0022214467	70.0	40
CTCGAAA	15	0.0022214467	70.0	57
TACCTCG	65	0.0	59.230774	36
CGTATGC	770	0.0	53.636364	41
CGTCTTC	755	0.0	53.311256	48
CTCGTAT	775	0.0	53.290325	39
TCGTATG	775	0.0	53.290325	40
CTTGAAA	730	0.0	53.21918	57
CCGTCTT	760	0.0	52.96053	47
TATGCCG	775	0.0	52.83871	43
TTGGACG	20	0.006941316	52.5	49
CGCATGC	20	0.006941316	52.5	41
TCGTAAC	20	0.006941316	52.5	17
CGTAACA	20	0.006941316	52.5	18
GTAAGTG	20	0.006941316	52.5	27
AGTGTAC	20	0.006941316	52.5	30
CTGCTTG	760	0.0	52.03948	54
GCCGTCT	775	0.0	51.935486	46
GTATGCC	800	0.0	51.187504	42
TCTCGTA	685	0.0	51.094887	38
>>END_MODULE