FastQCFastQC Report
Wed 25 May 2016
SRR1781206_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781206_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences176372
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCATAG150.002221773870.09
ACTCGAC150.002221773870.025
AGAGCCG150.002221773870.05
CTAGATC350.001252880740.021
ACAGACG350.001252880740.08
TTTACGC450.004298673531.1111092
TAGATCA450.004298673531.11110922
GCAATTT450.004298673531.11110914
ACGGTTA450.004298673531.11110950
ATTTAAG500.007194085528.00000218
AAATACG500.007194085528.00000213
GGCAATT500.007194085528.00000213
CTACGGA500.007194085528.00000224
TAGCTCG500.007194085528.00000210
TGGAGGC657.932501E-426.9230775
GAGCCGC657.932501E-426.9230776
TCTACGG808.629896E-526.2523
TCTATAC808.629896E-526.253
TTTCGGG700.001222440425.022
TTCCACC700.001222440425.036
Produced by FastQC (version 0.11.5)