##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1781206_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	176372
Sequences flagged as poor quality	0
Sequence length	76
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.0381012859184	34.0	32.0	34.0	21.0	34.0
2	31.18612364774454	34.0	32.0	34.0	23.0	34.0
3	31.18154242169959	34.0	33.0	34.0	23.0	34.0
4	31.363260608259814	34.0	33.0	34.0	23.0	34.0
5	31.36528473907423	34.0	33.0	34.0	23.0	34.0
6	34.07446193273309	37.0	34.0	38.0	24.0	38.0
7	33.984719796793144	37.0	34.0	38.0	24.0	38.0
8	33.70485111015354	37.0	34.0	38.0	23.0	38.0
9	34.09914272106683	37.0	34.0	38.0	24.0	38.0
10-11	33.960075862381785	37.0	34.0	38.0	23.0	38.0
12-13	33.91582280634114	37.0	34.0	38.0	23.0	38.0
14-15	33.81668575510852	37.0	34.0	38.0	22.5	38.0
16-17	33.786527906924	37.0	34.0	38.0	22.5	38.0
18-19	33.822069262694754	37.0	34.0	38.0	23.0	38.0
20-21	33.745373415281335	37.0	34.0	38.0	22.5	38.0
22-23	33.799123443630506	37.0	34.0	38.0	22.5	38.0
24-25	33.7927023563831	37.0	34.0	38.0	22.5	38.0
26-27	33.7225551674869	37.0	34.0	38.0	22.0	38.0
28-29	33.81779420769736	37.0	34.0	38.0	23.0	38.0
30-31	33.883961172975305	37.0	34.0	38.0	23.0	38.0
32-33	33.81589481323566	37.0	34.0	38.0	22.5	38.0
34-35	33.82276948722019	37.0	34.0	38.0	23.0	38.0
36-37	33.6903845281564	37.0	34.0	38.0	22.5	38.0
38-39	33.716899507858386	37.0	34.0	38.0	22.0	38.0
40-41	33.68675016442519	37.0	34.0	38.0	22.0	38.0
42-43	33.77528179076044	37.0	34.0	38.0	23.0	38.0
44-45	33.80058909577484	37.0	34.0	38.0	23.0	38.0
46-47	33.79552309890459	37.0	34.0	38.0	23.0	38.0
48-49	33.7512927222008	37.0	34.0	38.0	22.5	38.0
50-51	33.634822987775834	37.0	34.0	38.0	22.0	38.0
52-53	33.711258022815414	37.0	34.0	38.0	22.5	38.0
54-55	33.682597010863404	37.0	34.0	38.0	22.0	38.0
56-57	33.66532669584741	37.0	34.0	38.0	22.0	38.0
58-59	33.64474803256753	37.0	34.0	38.0	22.0	38.0
60-61	33.627338806613295	37.0	34.0	38.0	22.0	38.0
62-63	33.60345179506952	37.0	34.0	38.0	22.0	38.0
64-65	33.618133830766794	37.0	34.0	38.0	22.0	38.0
66-67	33.484898396570884	37.0	34.0	38.0	22.0	38.0
68-69	33.24556335472751	37.0	33.5	38.0	21.0	38.0
70-71	33.3804855646021	37.0	34.0	38.0	21.0	38.0
72-73	33.312025151384574	37.0	33.5	38.0	21.0	38.0
74-75	33.26952974395029	37.0	33.0	38.0	21.0	38.0
76	31.810757943437732	36.0	29.0	38.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	3.0
13	15.0
14	110.0
15	310.0
16	767.0
17	1343.0
18	1746.0
19	1959.0
20	1872.0
21	1722.0
22	1736.0
23	1907.0
24	2174.0
25	2499.0
26	2962.0
27	3547.0
28	4126.0
29	4930.0
30	6062.0
31	6959.0
32	8452.0
33	10340.0
34	13056.0
35	17369.0
36	25782.0
37	54622.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.9237974281632	18.22568208105595	10.001020570158529	26.84949992062232
2	23.26729866418706	18.984305898895517	31.776018869208265	25.97237656770916
3	20.207856122286984	21.128070215226906	28.48297915768943	30.18109450479668
4	14.456943278978523	15.830744109042252	36.34647222915202	33.36584038282721
5	14.396843036309617	33.45372281314494	35.30945955140272	16.83997459914272
6	31.25496110493729	35.57537477604155	18.01363028145057	15.15603383757059
7	30.111355543963896	28.010681967659266	22.011997369196926	19.865965119179915
8	26.628943369695868	32.62139115052276	19.775247771755154	20.974417708026216
9	25.07498398262753	15.447550901225258	21.386735914634492	38.090729201512715
10-11	26.202004853378085	23.6656045177239	27.095570725511987	23.036819903386025
12-13	25.68519946476765	22.011430385775522	26.433050597600527	25.8703195518563
14-15	23.94604570466317	23.40911088242715	23.413363270142852	29.23148014276683
16-17	24.480359694282537	25.587678316286034	24.857403669516703	25.074558319914726
18-19	23.79856213004332	25.54487106796997	26.037579661170707	24.618987140816003
20-21	23.808200848207196	25.710146735309458	25.790091397727533	24.691561018755813
22-23	24.24647903295308	25.096670673349514	26.373233846642325	24.28361644705509
24-25	23.68459846234096	25.44394801895993	26.13453382623092	24.73691969246819
26-27	23.10655886421881	25.705894359648923	26.64085002154537	24.546696754586897
28-29	23.855827455605198	25.38016238405189	27.017893996779534	23.74611616356338
30-31	24.06561132152496	25.227360351983307	26.253600344725918	24.453427981765813
32-33	23.424069580205476	25.618862404463293	26.275712698160707	24.681355317170524
34-35	24.292688181797566	25.048477082530106	26.34516816728279	24.313666568389543
36-37	24.310264667861112	24.769521239198966	26.05770757263058	24.862506520309346
38-39	23.689984804844304	25.365137323384662	26.87643163313905	24.06844623863198
40-41	23.783820561086795	24.465901617036717	27.43434331980133	24.31593450207516
42-43	23.29479736012519	24.742306034971538	27.086782482480213	24.87611412242306
44-45	22.90981561699136	24.35533984986279	27.41648334202708	25.318361191118772
46-47	22.901027373959586	24.04434944322228	27.933572222348218	25.121050960469915
48-49	22.302859864377567	24.21444446964371	28.563774295239607	24.91892137073912
50-51	22.186344771278886	24.00834599596308	28.348604086816504	25.456705145941534
52-53	21.83651600027215	24.038963100718934	28.72479758691856	25.399723312090355
54-55	20.939831719320527	24.771789172884585	28.75569818338512	25.532680924409775
56-57	20.68043680402785	24.80467421132606	28.551867643390104	25.96302134125598
58-59	21.26187830267843	24.704885129158825	27.97326106184655	26.059975506316196
60-61	20.853650239267004	24.438402921098586	28.16631891683487	26.541627922799538
62-63	20.688941555348922	23.966672714489828	28.494318826117528	26.85006690404372
64-65	21.60518676433901	24.18354387317715	27.571836799491983	26.63943256299186
66-67	21.595831537885832	24.082053840745697	27.095287233801287	27.226827387567187
68-69	21.29192842401288	24.19460004989454	26.798754904406596	27.71471662168598
70-71	21.983648198126687	24.24846347492799	26.379187172567075	27.388701154378246
72-73	21.7826525752387	23.788923411879438	26.430499172204204	27.99792484067766
74-75	21.84927312725376	23.908273422085138	26.2986755267276	27.943777923933506
76	22.228018052752137	23.986233642528294	26.240559726033613	27.54518857868596
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.5
2	0.5
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	1.0
14	1.5
15	5.5
16	14.0
17	18.0
18	18.5
19	31.0
20	45.0
21	47.0
22	79.5
23	154.0
24	249.5
25	303.0
26	401.5
27	557.5
28	736.0
29	857.0
30	1070.0
31	1419.0
32	2032.5
33	2510.0
34	2802.0
35	3518.0
36	4550.5
37	5159.0
38	5745.5
39	7213.0
40	8734.0
41	9914.5
42	10455.0
43	10545.5
44	10891.5
45	11171.5
46	11196.0
47	11113.5
48	10401.5
49	9337.0
50	8902.0
51	8282.0
52	6911.5
53	5642.5
54	5124.0
55	4799.0
56	4124.0
57	3332.5
58	2891.0
59	2668.0
60	2370.0
61	2149.5
62	2004.0
63	2000.0
64	1959.0
65	1859.0
66	1804.5
67	1813.0
68	1825.0
69	1785.5
70	1657.5
71	1581.0
72	1605.0
73	1574.5
74	1448.5
75	1377.0
76	1281.5
77	1083.5
78	872.0
79	763.0
80	714.5
81	575.5
82	421.0
83	357.0
84	313.0
85	206.0
86	128.0
87	113.0
88	94.5
89	57.5
90	28.5
91	17.5
92	17.0
93	10.5
94	3.5
95	3.0
96	3.0
97	3.0
98	2.5
99	2.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	5.669834214047582E-4
10-11	0.0
12-13	0.0
14-15	2.834917107023791E-4
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	176372.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.437302973261055
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.09112553873966	37.59610368992811
2	12.843773767925839	13.212981652416483
3	5.178514346182251	7.991064341278661
4	2.436040167105742	5.012133445218062
5	1.3458846353104574	3.4614337876760484
6	0.8509606375591098	2.6262672079468397
7	0.6856185447691273	2.468645816796317
8	0.5301969775465437	2.1817522055655094
9	0.44973049238875235	2.0819631233982716
>10	2.5628024382447285	22.518880547932778
>50	0.023147892990597545	0.7280067130837095
>100	0.002204561237199766	0.12076746875921349
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0011339668428095163	0.0
2	0.0	0.0	0.0	0.0011339668428095163	0.0
3	0.0	0.0	0.0	0.0011339668428095163	0.0
4	0.0	0.0	0.0	0.0011339668428095163	0.0
5	0.0	0.0	0.0	0.0022679336856190327	0.0
6	0.0	0.0	0.0	0.0022679336856190327	0.0
7	0.0	0.0	0.0	0.0022679336856190327	0.0
8	0.0	0.0	0.0	0.0022679336856190327	0.0
9	0.0	0.0	0.0	0.0028349171070237906	0.0
10	0.0	0.0	0.0	0.0028349171070237906	0.0
11	0.0	0.0	0.0	0.0028349171070237906	0.0
12	0.0	0.0	0.0	0.0034019005284285486	0.0
13	0.0	0.0	0.0	0.003968883949833307	0.0
14	0.0	0.0	0.0	0.003968883949833307	0.0
15	0.0	0.0	0.0	0.003968883949833307	0.0
16	0.0	0.0	0.0	0.003968883949833307	0.0
17	0.0	0.0	0.0	0.003968883949833307	0.0
18	0.0	0.0	0.0	0.003968883949833307	0.0
19	0.0	0.0	0.0	0.003968883949833307	0.0
20	0.0	0.0	0.0	0.003968883949833307	0.0
21	0.0	0.0	0.0	0.003968883949833307	0.0
22	0.0	0.0	0.0	0.005669834214047581	0.0
23	0.0	0.0	0.0	0.005669834214047581	0.0
24	0.0	0.0	0.0	0.008504751321071372	0.0
25	0.0	0.0	0.0	0.010205701585285646	0.0
26	0.0	0.0	0.0	0.011339668428095162	0.0
27	0.0	0.0	0.0	0.02381330369899984	0.0
28	0.0	0.0	0.0	0.03401900528428549	0.0
29	0.0	0.0	0.0	0.058399292404690085	0.0
30	0.0	0.0	0.0	0.08051164583947565	0.0
31	0.0	0.0	0.0	0.10319098269566598	0.0
32	0.0	0.0	0.0	0.1462817227224276	0.0
33	0.0	0.0	0.0	0.19050642959199873	0.0
34	0.0	0.0	0.0	0.28122377701676005	0.0
35	0.0	0.0	0.0	0.39121856076928313	0.0
36	0.0	0.0	0.0	0.564715487719139	0.0
37	0.0	0.0	0.0	0.8487741818429229	0.0
38	0.0	0.0	0.0	1.2184473725988252	0.0
39	0.0	0.0	0.0	1.6493547728664413	0.0
40	0.0	0.0	0.0	2.1534030344952715	0.0
41	0.0	0.0	0.0	2.7203864559000297	0.0
42	0.0	0.0	0.0	3.323089832853287	0.0
43	0.0	0.0	0.0	4.090218402013925	0.0
44	0.0	0.0	0.0	4.946930351756515	0.0
45	0.0	0.0	0.0	5.943120223164675	0.0
46	0.0	0.0	0.0	6.91606377429524	0.0
47	0.0	0.0	0.0	7.939468849930828	0.0
48	0.0	0.0	0.0	8.93339078765337	0.0
49	0.0	0.0	0.0	9.84396616242941	0.0
50	0.0	0.0	0.0	10.705213979543238	0.0
51	0.0	0.0	0.0	11.576100514820947	0.0
52	0.0	0.0	0.0	12.43451341482775	0.0
53	0.0	0.0	0.0	13.284421563513483	0.0
54	0.0	0.0	0.0	14.208604540403238	0.0
55	0.0	0.0	0.0	15.102737395958542	0.0
56	0.0	0.0	0.0	16.03826004127639	0.0
57	0.0	0.0	0.0	16.927290046039055	0.0
58	0.0	0.0	0.0	17.819154967908737	0.0
59	0.0	0.0	0.0	18.610663824189782	0.0
60	0.0	0.0	0.0	19.420316149955774	0.0
61	0.0	0.0	0.0	20.18857868595922	0.0
62	0.0	0.0	0.0	20.94266663642755	0.0
63	0.0	0.0	0.0	21.72793867507314	0.0
64	0.0	0.0	0.0	22.47578980790602	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCATAG	15	0.0022217738	70.0	9
ACTCGAC	15	0.0022217738	70.0	25
AGAGCCG	15	0.0022217738	70.0	5
CTAGATC	35	0.0012528807	40.0	21
ACAGACG	35	0.0012528807	40.0	8
TTTACGC	45	0.0042986735	31.111109	2
TAGATCA	45	0.0042986735	31.111109	22
GCAATTT	45	0.0042986735	31.111109	14
ACGGTTA	45	0.0042986735	31.111109	50
ATTTAAG	50	0.0071940855	28.000002	18
AAATACG	50	0.0071940855	28.000002	13
GGCAATT	50	0.0071940855	28.000002	13
CTACGGA	50	0.0071940855	28.000002	24
TAGCTCG	50	0.0071940855	28.000002	10
TGGAGGC	65	7.932501E-4	26.923077	5
GAGCCGC	65	7.932501E-4	26.923077	6
TCTACGG	80	8.629896E-5	26.25	23
TCTATAC	80	8.629896E-5	26.25	3
TTTCGGG	70	0.0012224404	25.0	22
TTCCACC	70	0.0012224404	25.0	36
>>END_MODULE