FastQCFastQC Report
Wed 25 May 2016
SRR1781206_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781206_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences159665
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCGT42552.6649547490057306No Hit
TCTTATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCG4130.2586665831584881No Hit
CTTATACACATCTCCGAGCCCACGAGACAAATACAGACCTCGTATGCCGT2980.18664077913130617No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCC2760.17286192966523659No Hit
CTTATACACATCTCCGAGCCCACGAGACAAATACAGAACTCGTATGCCGT2520.1578304575204334No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTGAAA6700.057.98507357
ATCTCGT6250.057.6837
TCGTATG7100.057.183140
CGTATGC7150.056.78321541
TATGCCG7150.056.78321543
TCTCGTA6350.056.77165238
CCGTCTT6750.056.51851747
CTCGTAT7200.056.38888539
TTGAAAA6750.056.058
GTATGCC7300.055.61643642
CGTCTTC6800.055.58823448
AGATCTC6550.055.57251735
ACAGATC6450.055.3488433
GATCTCG6550.055.03816636
CAGATCT6500.054.92307734
ATGCCGT7350.054.761944
TGCCGTC7250.054.55172345
CTGCTTG7200.054.44444354
GCCGTCT7150.053.84615346
TCTTCTG7050.053.12056450