FastQCFastQC Report
Wed 25 May 2016
SRR1781205_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781205_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences420238
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTACGA207.922774E-570.01
GCGGTTT601.2147135E-535.00000440
CAACGTC901.7542152E-731.11110916
TAGCGCT450.004305382331.11110932
AACGTCA1004.4133958E-727.99999817
TTAGCGC500.007205247427.99999831
TCAACGT1004.4133958E-727.99999815
ACGTCAA906.163351E-627.22222118
GTACAAG657.950888E-426.9230771
CGTACTA657.950888E-426.92307736
GCACTTA657.950888E-426.9230771
TTTGCGG808.657832E-526.2537
ACTCGAT959.379344E-625.7894736
CTTAGCG700.001225259725.030
CCGTGCG851.3070901E-424.7058839
CGCAGGT1001.3960187E-524.49999830
CTCGATT1001.3960187E-524.4999987
TTTCGAT750.001831105123.33333444
GAGTCGC1052.0366395E-523.33333226
TCGATTG1052.0366395E-523.3333328
Produced by FastQC (version 0.11.5)