##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1781205_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	420238
Sequences flagged as poor quality	0
Sequence length	76
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.422348764271675	34.0	32.0	34.0	23.0	34.0
2	31.312922677149615	34.0	32.0	34.0	23.0	34.0
3	31.304993836825798	34.0	33.0	34.0	23.0	34.0
4	31.48966062088626	34.0	33.0	34.0	23.0	34.0
5	31.47509982438523	34.0	33.0	34.0	23.0	34.0
6	34.284303180578625	37.0	34.0	38.0	24.0	38.0
7	34.15273963801465	37.0	34.0	38.0	24.0	38.0
8	33.98313574688629	37.0	34.0	38.0	24.0	38.0
9	34.31300120407959	37.0	34.0	38.0	24.0	38.0
10-11	34.17164559130778	37.0	34.0	38.0	24.0	38.0
12-13	34.125552663014766	37.0	34.0	38.0	24.0	38.0
14-15	33.99591184043328	37.0	34.0	38.0	23.5	38.0
16-17	33.97183976698918	37.0	34.0	38.0	23.5	38.0
18-19	34.03830805400749	37.0	34.0	38.0	23.5	38.0
20-21	33.96571942565879	37.0	34.0	38.0	23.5	38.0
22-23	34.04290306921316	37.0	34.0	38.0	23.5	38.0
24-25	34.036951679762424	37.0	34.0	38.0	23.5	38.0
26-27	33.94955001689519	37.0	34.0	38.0	23.0	38.0
28-29	34.034805277009696	37.0	34.0	38.0	24.0	38.0
30-31	34.126099971920674	37.0	34.0	38.0	24.0	38.0
32-33	34.07370585239793	37.0	34.0	38.0	24.0	38.0
34-35	34.100853563932816	37.0	34.0	38.0	24.0	38.0
36-37	33.958226052855764	37.0	34.0	38.0	23.5	38.0
38-39	33.9697457155231	37.0	34.0	38.0	23.0	38.0
40-41	33.95778225672119	37.0	34.0	38.0	23.5	38.0
42-43	34.042636553572024	37.0	34.0	38.0	24.0	38.0
44-45	34.05365649941224	37.0	34.0	38.0	24.0	38.0
46-47	34.043045845449484	37.0	34.0	38.0	24.0	38.0
48-49	34.00914719754044	37.0	34.0	38.0	24.0	38.0
50-51	33.893989834331975	37.0	34.0	38.0	23.0	38.0
52-53	33.97482973933819	37.0	34.0	38.0	24.0	38.0
54-55	33.93570310157577	37.0	34.0	38.0	23.5	38.0
56-57	33.91456745939206	37.0	34.0	38.0	23.5	38.0
58-59	33.91556094403647	37.0	34.0	38.0	23.5	38.0
60-61	33.89808037350264	37.0	34.0	38.0	23.5	38.0
62-63	33.87139668473579	37.0	34.0	38.0	23.0	38.0
64-65	33.87847481665152	37.0	34.0	38.0	23.0	38.0
66-67	33.767137907566664	37.0	34.0	38.0	23.0	38.0
68-69	33.5707051718312	37.0	34.0	38.0	22.5	38.0
70-71	33.72366492321018	37.0	34.0	38.0	23.0	38.0
72-73	33.67811692421913	37.0	34.0	38.0	23.0	38.0
74-75	33.67253080397299	37.0	34.0	38.0	23.0	38.0
76	32.34293186242082	37.0	31.0	38.0	21.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	4.0
13	29.0
14	115.0
15	336.0
16	793.0
17	1594.0
18	2400.0
19	2853.0
20	3130.0
21	3588.0
22	4000.0
23	4706.0
24	5615.0
25	6536.0
26	7859.0
27	8968.0
28	10730.0
29	12636.0
30	14631.0
31	17798.0
32	20957.0
33	25252.0
34	31372.0
35	41020.0
36	60958.0
37	132356.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	48.0984584925685	18.37649141676859	9.075571461885884	24.449478628777026
2	23.889557821996107	20.54074119903483	30.533649979297444	25.036050999671616
3	19.487528495757168	22.060118313907832	30.212403447570185	28.239949742764814
4	14.062745396656181	16.957533588109595	37.352405065700864	31.627315949533358
5	13.778858646766832	33.67758270313489	36.126195156078225	16.417363494020055
6	29.107553338822285	37.44449573812935	19.2855001213598	14.162450801688568
7	28.80796120293738	29.746239035974853	22.30688324235314	19.13891651873462
8	25.648799013892127	34.45190582479452	20.316106587219622	19.583188574093725
9	24.909694030525557	16.690066105397417	21.87474716708151	36.52549269699551
10-11	25.01856091072202	24.664356864443484	28.174272674056127	22.14280955077837
12-13	25.39299159047968	23.107857928126442	27.010170427234094	24.488980054159786
14-15	23.45099681608993	24.256254788952926	24.383682579871408	27.909065815085736
16-17	23.530832528234	27.39090705742936	25.451291886978332	23.626968527358304
18-19	22.81361990110366	27.035156268590654	27.013025951960557	23.138197878345128
20-21	22.920107177361402	27.11856138664281	26.731875746600736	23.229455689395056
22-23	23.179959927469675	26.605399797257746	27.09571718883109	23.11892308644149
24-25	22.990543453947527	26.727235518920228	27.030159100319345	23.2520619268129
26-27	22.348407331083813	26.91320156673123	27.167343267386578	23.571047834798375
28-29	22.84384087112541	26.81099757756319	27.75974566793103	22.58541588338037
30-31	23.066571799789642	26.742227023734173	27.28239711782371	22.90880405865248
32-33	22.512362042461653	27.357711582484214	26.890952269904194	23.23897410514994
34-35	23.43065120241387	26.250362889600652	27.34367191924576	22.975313988739714
36-37	23.298940124405693	26.503433768483575	26.781966409510797	23.41565969759993
38-39	22.689642535896326	26.92343386366773	27.512623798894914	22.87429980154103
40-41	22.821710554495308	26.115796286866015	27.838510558302676	23.223982600335997
42-43	22.643835160075955	26.41074819507041	27.586391520995246	23.359025123858384
44-45	22.128056006358303	26.139949266844027	27.869326429309105	23.862668297488565
46-47	22.321517806576274	25.975280674284573	27.987949685654318	23.715251833484835
48-49	21.768140910626833	25.782532755248216	28.67351358039968	23.77581275372527
50-51	21.84690580099848	25.84666308139673	28.29551349473394	24.01091762287085
52-53	21.425596923648026	25.77610782461367	28.974771439041685	23.823523812696614
54-55	21.019517511505384	26.20610225634045	28.820930044403408	23.95345018775075
56-57	20.90349232336116	26.065472734368306	28.700777658268212	24.33025728400232
58-59	20.87272597475222	26.12324056777755	28.603400478303808	24.40063297916642
60-61	20.848785978338334	25.929984496788112	28.53221586467588	24.689013660197677
62-63	20.522277958099735	25.696989310768238	28.972767682921024	24.807965048211006
64-65	21.113850312681446	25.825131592724087	28.31646979316384	24.744548301430626
66-67	21.06908847755966	25.701004077465907	27.92510883752363	25.304798607450806
68-69	20.866489306009004	25.676048696446756	27.774393436069257	25.683068561474983
70-71	21.507702228869288	25.565070061905764	27.53586976826089	25.391357940964056
72-73	21.428554431319544	25.192986797894516	27.443032010584528	25.935426760201413
74-75	21.232304628102714	25.408519478294387	27.40679664094309	25.952379252659806
76	21.308877350453788	25.480323055030716	27.20886735611725	26.00193223839824
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.5
4	1.0
5	0.5
6	0.0
7	0.0
8	0.0
9	0.0
10	2.0
11	2.5
12	1.0
13	8.0
14	19.0
15	36.5
16	84.0
17	118.0
18	132.5
19	202.5
20	350.5
21	443.0
22	534.5
23	799.5
24	1257.5
25	1542.0
26	1856.0
27	2385.0
28	2980.5
29	3361.0
30	3903.0
31	4887.5
32	6506.0
33	7682.0
34	8509.0
35	10519.0
36	13033.0
37	14364.0
38	15525.0
39	18492.5
40	21758.5
41	24129.5
42	25041.0
43	25700.0
44	26779.5
45	26970.5
46	26741.0
47	26422.5
48	24707.5
49	22242.5
50	21174.0
51	19819.5
52	16703.0
53	13906.5
54	12872.0
55	11973.0
56	10106.5
57	8263.0
58	7387.0
59	6524.0
60	5148.0
61	4314.5
62	3994.0
63	3703.5
64	3218.0
65	2915.5
66	2741.5
67	2675.0
68	2555.5
69	2425.5
70	2319.0
71	2223.0
72	2101.0
73	1938.0
74	1796.0
75	1695.0
76	1564.0
77	1294.5
78	1089.0
79	1022.0
80	912.5
81	690.0
82	493.5
83	410.0
84	350.5
85	237.0
86	147.5
87	112.0
88	95.0
89	62.5
90	37.5
91	19.5
92	11.0
93	9.5
94	9.0
95	7.0
96	4.0
97	3.0
98	1.0
99	3.5
100	7.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	4.7592078774408785E-4
58-59	7.138811816161318E-4
60-61	5.949009846801099E-4
62-63	4.7592078774408785E-4
64-65	4.7592078774408785E-4
66-67	3.569405908080659E-4
68-69	4.7592078774408785E-4
70-71	5.949009846801099E-4
72-73	4.7592078774408785E-4
74-75	2.3796039387204393E-4
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	420238.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.88877789802865
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.57477613222146	35.48092111327326
2	13.997877397611616	13.686782382713394
3	5.563188553277254	8.15932468758081
4	2.2997308981508486	4.4972413241972315
5	1.0563638216822064	2.5822168128867067
6	0.6728342050319578	1.9736425207202435
7	0.4693722212882902	1.6062923994647302
8	0.38138486286948686	1.4916351883597168
9	0.3137836861465086	1.3806450846037248
>10	2.5217211107347404	24.1135260932519
>50	0.13724510880969218	4.374228840541093
>100	0.011722002175900395	0.6535435524072029
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	4.7592078774408785E-4	0.0
3	0.0	0.0	0.0	4.7592078774408785E-4	0.0
4	0.0	0.0	0.0	0.0011898019693602196	0.0
5	0.0	0.0	0.0	0.0014277623632322637	0.0
6	0.0	0.0	0.0	0.0014277623632322637	0.0
7	0.0	0.0	0.0	0.0014277623632322637	0.0
8	0.0	0.0	0.0	0.0014277623632322637	0.0
9	0.0	0.0	0.0	0.0014277623632322637	0.0
10	0.0	0.0	0.0	0.0014277623632322637	0.0
11	0.0	0.0	0.0	0.0016657227571043075	0.0
12	0.0	0.0	0.0	0.0016657227571043075	0.0
13	0.0	0.0	0.0	0.0019036831509763514	0.0
14	0.0	0.0	0.0	0.0019036831509763514	0.0
15	0.0	0.0	4.7592078774408785E-4	0.0019036831509763514	0.0
16	0.0	0.0	4.7592078774408785E-4	0.0019036831509763514	0.0
17	0.0	0.0	4.7592078774408785E-4	0.0021416435448483955	0.0
18	0.0	0.0	4.7592078774408785E-4	0.0021416435448483955	0.0
19	0.0	0.0	4.7592078774408785E-4	0.0028555247264645273	0.0
20	0.0	0.0	4.7592078774408785E-4	0.003331445514208615	0.0
21	0.0	0.0	4.7592078774408785E-4	0.003331445514208615	0.0
22	0.0	0.0	4.7592078774408785E-4	0.004283287089696791	0.0
23	0.0	0.0	4.7592078774408785E-4	0.004283287089696791	0.0
24	0.0	0.0	4.7592078774408785E-4	0.0052351286651849665	0.0
25	0.0	0.0	4.7592078774408785E-4	0.006186970240673142	0.0
26	0.0	0.0	4.7592078774408785E-4	0.00666289102841723	0.0
27	0.0	0.0	4.7592078774408785E-4	0.011660059299730153	0.0
28	0.0	0.0	4.7592078774408785E-4	0.019512752297507603	0.0
29	0.0	0.0	4.7592078774408785E-4	0.031886692778853885	0.0
30	0.0	0.0	4.7592078774408785E-4	0.04545043522956039	0.0
31	0.0	0.0	4.7592078774408785E-4	0.06163174201285938	0.0
32	0.0	0.0	4.7592078774408785E-4	0.08661758336942399	0.0
33	0.0	0.0	4.7592078774408785E-4	0.11874223654214992	2.3796039387204393E-4
34	0.0	0.0	4.7592078774408785E-4	0.16871391925527915	2.3796039387204393E-4
35	0.0	0.0	4.7592078774408785E-4	0.22653829496618583	2.3796039387204393E-4
36	0.0	0.0	4.7592078774408785E-4	0.3326686306331174	2.3796039387204393E-4
37	0.0	0.0	4.7592078774408785E-4	0.5085213617045579	2.3796039387204393E-4
38	0.0	0.0	4.7592078774408785E-4	0.7455299140011137	2.3796039387204393E-4
39	0.0	0.0	4.7592078774408785E-4	1.012045555137803	2.3796039387204393E-4
40	0.0	0.0	4.7592078774408785E-4	1.33186432450183	2.3796039387204393E-4
41	0.0	0.0	4.7592078774408785E-4	1.6883289945221518	2.3796039387204393E-4
42	0.0	0.0	4.7592078774408785E-4	2.105235604585973	2.3796039387204393E-4
43	0.0	0.0	4.7592078774408785E-4	2.5878192833584777	2.3796039387204393E-4
44	0.0	0.0	4.7592078774408785E-4	3.162969555347208	2.3796039387204393E-4
45	0.0	0.0	4.7592078774408785E-4	3.8007034109242857	2.3796039387204393E-4
46	0.0	0.0	4.7592078774408785E-4	4.486981186851261	2.3796039387204393E-4
47	0.0	0.0	4.7592078774408785E-4	5.213712229736482	2.3796039387204393E-4
48	0.0	0.0	4.7592078774408785E-4	5.914505589689652	2.3796039387204393E-4
49	0.0	0.0	4.7592078774408785E-4	6.620534078308006	2.3796039387204393E-4
50	0.0	0.0	4.7592078774408785E-4	7.252556884432155	2.3796039387204393E-4
51	0.0	0.0	4.7592078774408785E-4	7.8650669382587965	2.3796039387204393E-4
52	0.0	0.0	4.7592078774408785E-4	8.477576992085437	2.3796039387204393E-4
53	0.0	0.0	4.7592078774408785E-4	9.119832095146084	2.3796039387204393E-4
54	0.0	0.0	4.7592078774408785E-4	9.799922900832385	2.3796039387204393E-4
55	0.0	0.0	4.7592078774408785E-4	10.521894735840167	2.3796039387204393E-4
56	0.0	0.0	4.7592078774408785E-4	11.212217838462966	2.3796039387204393E-4
57	0.0	0.0	4.7592078774408785E-4	11.896829891632837	2.3796039387204393E-4
58	0.0	0.0	4.7592078774408785E-4	12.571923529047826	2.3796039387204393E-4
59	0.0	0.0	4.7592078774408785E-4	13.229408097316282	2.3796039387204393E-4
60	0.0	0.0	4.7592078774408785E-4	13.879753853768579	2.3796039387204393E-4
61	0.0	0.0	4.7592078774408785E-4	14.465850303875422	2.3796039387204393E-4
62	0.0	0.0	4.7592078774408785E-4	15.05218471437614	2.3796039387204393E-4
63	0.0	0.0	4.7592078774408785E-4	15.6449440555114	2.3796039387204393E-4
64	0.0	0.0	4.7592078774408785E-4	16.27101785178875	2.3796039387204393E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTACGA	20	7.922774E-5	70.0	1
GCGGTTT	60	1.2147135E-5	35.000004	40
CAACGTC	90	1.7542152E-7	31.111109	16
TAGCGCT	45	0.0043053823	31.111109	32
AACGTCA	100	4.4133958E-7	27.999998	17
TTAGCGC	50	0.0072052474	27.999998	31
TCAACGT	100	4.4133958E-7	27.999998	15
ACGTCAA	90	6.163351E-6	27.222221	18
GTACAAG	65	7.950888E-4	26.923077	1
CGTACTA	65	7.950888E-4	26.923077	36
GCACTTA	65	7.950888E-4	26.923077	1
TTTGCGG	80	8.657832E-5	26.25	37
ACTCGAT	95	9.379344E-6	25.789473	6
CTTAGCG	70	0.0012252597	25.0	30
CCGTGCG	85	1.3070901E-4	24.705883	9
CGCAGGT	100	1.3960187E-5	24.499998	30
CTCGATT	100	1.3960187E-5	24.499998	7
TTTCGAT	75	0.0018311051	23.333334	44
GAGTCGC	105	2.0366395E-5	23.333332	26
TCGATTG	105	2.0366395E-5	23.333332	8
>>END_MODULE