FastQCFastQC Report
Wed 25 May 2016
SRR1781205_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781205_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences376051
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCCGT36650.9746018492172603No Hit
TCTTATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCCG4310.11461211378243907No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGAACCG150.002223737370.05
CAACTCG401.0646545E-861.2536
TCGTATG6050.058.42975240
CGTATGC6200.058.1451641
TATGCCG6150.058.0487843
CTCGTAT6000.057.16666839
TCTCGTA5000.056.038
GCCGTCT6150.055.77235846
GTATGCC6550.055.03816642
ATCTCGT5100.054.9019637
CGTCTTC6200.054.75806448
CCGTCTT6300.053.88888547
CATCTCG5200.053.84615336
TAAACGG200.00694841852.521
CGAGCTA200.00694841852.523
ATGCCGT6700.052.23880844
TGCCGTC6850.051.0948945
GCATCTC5500.050.9090935
TCTTCTG6700.049.62686550
AACTCGT506.186565E-848.99999637
Produced by FastQC (version 0.11.5)