FastQCFastQC Report
Wed 25 May 2016
SRR1781204_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781204_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences304382
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGAGA200.006947151452.557
TGCACGG501.7075357E-435.00000447
ACGGTTA501.7075357E-435.00000450
ATTCGGG400.00241664935.045
CTTTGCG400.00241664935.038
CATCGTT601.213615E-535.032
GGCGCTA652.0989723E-532.30769318
TGCTACC552.9883228E-431.81818438
ACCTTTG552.9883228E-431.81818442
TGCCGAG552.9883228E-431.81818457
CTCGTAA450.004303533631.11111329
ATTCGCA450.004303533631.1111136
CACTACG450.004303533631.1111134
CGTCCCG450.004303533631.11111335
GCCAACT450.004303533631.11111316
CTCAACG802.4607434E-630.62546
TAACCGG802.4607434E-630.62522
TCGTTGT604.975788E-429.16666634
CGGCTAC853.946432E-628.8235351
ACGTTGG500.007202172728.00000228