##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1781204_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	304382
Sequences flagged as poor quality	0
Sequence length	76
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.340302646017175	34.0	32.0	34.0	23.0	34.0
2	31.346078283209913	34.0	33.0	34.0	23.0	34.0
3	31.35287894816382	34.0	33.0	34.0	23.0	34.0
4	31.483711257564508	34.0	33.0	34.0	23.0	34.0
5	31.54058387158242	34.0	33.0	34.0	23.0	34.0
6	34.44223377203645	37.0	34.0	38.0	26.0	38.0
7	34.36092147367453	37.0	34.0	38.0	24.0	38.0
8	34.08685467603209	37.0	34.0	38.0	24.0	38.0
9	34.40414676294919	37.0	34.0	38.0	25.0	38.0
10-11	34.2620818576657	37.0	34.0	38.0	24.0	38.0
12-13	34.221658968007304	37.0	34.0	38.0	24.0	38.0
14-15	34.10801394300583	37.0	34.0	38.0	24.0	38.0
16-17	34.08012628867673	37.0	34.0	38.0	23.5	38.0
18-19	34.12669934490213	37.0	34.0	38.0	24.0	38.0
20-21	34.04523263530695	37.0	34.0	38.0	23.5	38.0
22-23	34.13078795723794	37.0	34.0	38.0	24.0	38.0
24-25	34.11987239718512	37.0	34.0	38.0	24.0	38.0
26-27	34.06053084610785	37.0	34.0	38.0	24.0	38.0
28-29	34.146749807807296	37.0	34.0	38.0	24.0	38.0
30-31	34.23665164168709	37.0	34.0	38.0	24.0	38.0
32-33	34.17235250441879	37.0	34.0	38.0	24.0	38.0
34-35	34.1855300247715	37.0	34.0	38.0	24.0	38.0
36-37	34.041799449376114	37.0	34.0	38.0	23.5	38.0
38-39	34.06747442358615	37.0	34.0	38.0	24.0	38.0
40-41	34.042658567195176	37.0	34.0	38.0	24.0	38.0
42-43	34.12898923063781	37.0	34.0	38.0	24.0	38.0
44-45	34.14292730844794	37.0	34.0	38.0	24.0	38.0
46-47	34.14066534814805	37.0	34.0	38.0	24.0	38.0
48-49	34.102678542095134	37.0	34.0	38.0	24.0	38.0
50-51	33.973526686860595	37.0	34.0	38.0	23.5	38.0
52-53	34.04968592098088	37.0	34.0	38.0	24.0	38.0
54-55	34.02574560913589	37.0	34.0	38.0	24.0	38.0
56-57	33.99694134344344	37.0	34.0	38.0	24.0	38.0
58-59	33.98746312199802	37.0	34.0	38.0	24.0	38.0
60-61	33.96493058065194	37.0	34.0	38.0	24.0	38.0
62-63	33.956017438613316	37.0	34.0	38.0	23.5	38.0
64-65	33.96819785664067	37.0	34.0	38.0	24.0	38.0
66-67	33.86013463345402	37.0	34.0	38.0	23.0	38.0
68-69	33.64429401212949	37.0	34.0	38.0	22.5	38.0
70-71	33.80734570375384	37.0	34.0	38.0	23.0	38.0
72-73	33.746371336018555	37.0	34.0	38.0	23.0	38.0
74-75	33.72191193960221	37.0	34.0	38.0	23.0	38.0
76	32.330873704752584	37.0	31.0	38.0	21.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	5.0
13	24.0
14	102.0
15	265.0
16	670.0
17	1243.0
18	1677.0
19	2034.0
20	2138.0
21	2432.0
22	2691.0
23	3174.0
24	3692.0
25	4460.0
26	5282.0
27	6364.0
28	7431.0
29	8684.0
30	10466.0
31	12898.0
32	15194.0
33	18221.0
34	23245.0
35	30236.0
36	45619.0
37	96133.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.45281258418697	18.89993495016131	9.96510963197561	24.682142833676103
2	23.724464652968965	20.538993764414453	31.007746844425753	24.728794738190825
3	19.319145021716132	22.09329066764789	29.97713399609701	28.610430314538966
4	13.996556958032999	16.889960641562247	37.09187796913089	32.02160443127386
5	13.538908345434356	33.955358726862954	36.19793548895795	16.307797438744736
6	29.625602039542414	36.97557674238293	18.963342116156674	14.435479101917984
7	29.373287513716317	29.278341031992696	22.48950332148419	18.8588681328068
8	25.9154614924667	34.00989546030974	20.115184209315924	19.95945883790763
9	24.775118108166712	17.54735825377322	21.99801565138543	35.67950798667464
10-11	25.428244771372814	24.709411200399497	27.464501843078764	22.397842185148924
12-13	25.56392953591211	23.03470638868264	26.88841652922972	24.51294754617553
14-15	23.592393768356867	24.14449606087088	24.111478339717856	28.151631831054395
16-17	23.845036828721803	27.106070661208616	25.37502217608137	23.673870333988212
18-19	23.121439506935364	26.72677753612237	26.75289603195984	23.398886924982424
20-21	23.04883337385259	26.909442739715228	26.786735089459956	23.254988796972224
22-23	23.41761339369608	26.370810363293494	27.174405845286515	23.037170397723912
24-25	22.841363812577615	26.793798582044932	27.09802156500713	23.266816040370326
26-27	22.5133220755498	26.900736574436067	27.273459008745586	23.312482341268538
28-29	23.077087344192496	26.58057966634032	27.890282605410306	22.452050384056875
30-31	23.38508847435131	26.493189479009928	27.082416174412415	23.039305872226347
32-33	22.683831501205724	26.93260442470317	26.959544256887725	23.42401981720338
34-35	23.693746673587796	26.22724076982213	27.211365980905573	22.867646575684503
36-37	23.611941573417614	26.126709200938297	26.716757232687872	23.54459199295622
38-39	22.974420300806223	26.63150251985991	27.46318770492342	22.930889474410446
40-41	23.197823787214748	25.72671839990538	27.88321911282533	23.19223870005454
42-43	22.604326142807395	26.08202850365659	27.60265061665933	23.710994736876685
44-45	22.16162585172579	26.087613590816805	27.840016820968387	23.910743736489017
46-47	22.478004612624925	25.503479180766277	28.179392999586046	23.839123207022755
48-49	21.772148155935632	25.685323048012037	28.84434690619025	23.69818188986208
50-51	21.838840667319356	25.54881694712565	28.531253490679475	24.081088894875517
52-53	21.519176561031863	25.303894448423364	28.954898778508586	24.22203021203619
54-55	20.861121879743216	25.741502454153004	29.060194098205542	24.337181567898234
56-57	20.64406228388481	25.946635871877472	28.995122880736446	24.414178963501275
58-59	21.00423317448662	25.67271664013746	28.638895596480076	24.684154588895844
60-61	20.627635475991397	25.475350753415544	29.026005279576054	24.871008491017
62-63	20.153622750359744	25.445164300122876	29.10651746818143	25.294695481335953
64-65	21.06169221570264	25.59349764440736	28.36419367768133	24.980616462208673
66-67	20.91762982042302	25.420524209710166	28.077547292546868	25.584298677319943
68-69	20.659073138359034	25.238844609733825	27.87976950016755	26.22231275173959
70-71	21.342823632224086	25.32451105686623	27.59699192787986	25.735673383029823
72-73	21.31696355237826	24.916880761674477	27.403722953394087	26.362432732553177
74-75	21.208382887292938	25.103816914272198	27.26196029988633	26.425839898548535
76	21.451991247839885	24.988665558410155	26.992726245310166	26.56661694843979
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.5
7	0.5
8	0.0
9	0.5
10	1.0
11	2.0
12	3.0
13	4.5
14	9.5
15	19.5
16	45.0
17	64.0
18	83.0
19	142.0
20	208.0
21	234.0
22	276.0
23	427.0
24	714.0
25	892.0
26	1056.0
27	1463.0
28	1953.5
29	2201.0
30	2564.0
31	3276.0
32	4437.0
33	5249.0
34	5835.0
35	7297.5
36	9136.5
37	10099.0
38	11108.5
39	13606.0
40	16265.0
41	18363.5
42	19291.0
43	19557.5
44	19939.0
45	20045.0
46	20036.0
47	19558.5
48	18120.0
49	16301.5
50	15444.0
51	14385.5
52	11923.5
53	9700.5
54	8881.0
55	8284.5
56	6821.0
57	5311.0
58	4668.0
59	4127.5
60	3400.5
61	2994.5
62	2775.0
63	2609.0
64	2321.5
65	2201.0
66	2101.0
67	2000.0
68	2033.5
69	1927.5
70	1717.0
71	1646.0
72	1611.0
73	1531.5
74	1454.5
75	1422.0
76	1353.5
77	1186.5
78	1027.5
79	967.0
80	804.0
81	550.5
82	399.0
83	338.0
84	285.5
85	191.0
86	119.0
87	89.0
88	73.5
89	49.0
90	30.5
91	17.0
92	13.0
93	9.5
94	4.0
95	3.0
96	4.0
97	2.0
98	1.0
99	2.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	4.928018082541017E-4
58-59	1.6426726941803392E-4
60-61	4.928018082541017E-4
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	3.2853453883606784E-4
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	304382.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.596267929536076
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.8974128829069	34.69644794944852
2	13.020827399500023	12.394855791416951
3	5.12929838582599	7.324063807869321
4	2.2651526768667503	4.312512548378228
5	1.2149042370477405	2.8912453787626435
6	0.8431600883184098	2.40787640826566
7	0.6306110758626351	2.1010313608263687
8	0.5016998714609044	1.9103233201813614
9	0.382181502746543	1.6371371882193466
>10	2.996122700751338	26.577262933878593
>50	0.11242183796619906	3.3930360824016543
>100	0.006207340746599949	0.35420723035133367
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	3.2853453883606784E-4	0.0
2	0.0	0.0	0.0	3.2853453883606784E-4	0.0
3	0.0	0.0	0.0	3.2853453883606784E-4	0.0
4	0.0	0.0	0.0	0.0013141381553442714	0.0
5	0.0	0.0	0.0	0.0013141381553442714	0.0
6	0.0	0.0	0.0	0.0013141381553442714	0.0
7	0.0	0.0	0.0	0.0013141381553442714	0.0
8	0.0	0.0	0.0	0.0013141381553442714	0.0
9	0.0	0.0	0.0	0.0013141381553442714	0.0
10	0.0	0.0	0.0	0.002299741771852475	0.0
11	0.0	0.0	0.0	0.0029568108495246106	0.0
12	0.0	0.0	0.0	0.0032853453883606785	0.0
13	0.0	0.0	0.0	0.0032853453883606785	0.0
14	0.0	0.0	0.0	0.0032853453883606785	0.0
15	0.0	0.0	0.0	0.0032853453883606785	0.0
16	0.0	0.0	0.0	0.0032853453883606785	0.0
17	0.0	0.0	0.0	0.0032853453883606785	0.0
18	0.0	0.0	0.0	0.0032853453883606785	0.0
19	0.0	0.0	0.0	0.003613879927196746	0.0
20	0.0	0.0	0.0	0.003942414466032814	0.0
21	0.0	0.0	0.0	0.00459948354370495	0.0
22	0.0	0.0	0.0	0.00459948354370495	0.0
23	0.0	0.0	0.0	0.00459948354370495	0.0
24	0.0	0.0	0.0	0.007227759854393492	0.0
25	0.0	0.0	0.0	0.00755629439322956	0.0
26	0.0	0.0	0.0	0.00755629439322956	0.0
27	0.0	0.0	0.0	0.01642672694180339	0.0
28	0.0	0.0	0.0	0.02464009041270509	0.0
29	0.0	0.0	0.0	0.03515319565545926	0.0
30	0.0	0.0	0.0	0.05420819890795119	0.0
31	0.0	0.0	0.0	0.06800664953906604	0.0
32	0.0	0.0	0.0	0.09231820541293506	0.0
33	0.0	0.0	0.0	0.12287191752468937	0.0
34	0.0	0.0	0.0	0.17806572004914878	0.0
35	0.0	0.0	0.0	0.25888521660282143	0.0
36	0.0	0.0	0.0	0.3870136867488879	0.0
37	0.0	0.0	0.0	0.5690218212640695	0.0
38	0.0	0.0	0.0	0.8049096201483662	0.0
39	0.0	0.0	0.0	1.104204585028024	0.0
40	0.0	0.0	0.0	1.453108265271928	0.0
41	0.0	0.0	0.0	1.8664047151277015	0.0
42	0.0	0.0	0.0	2.344093934595344	0.0
43	0.0	0.0	0.0	2.91672963578661	0.0
44	0.0	0.0	0.0	3.5806979387743034	0.0
45	0.0	0.0	0.0	4.358996261276948	0.0
46	0.0	0.0	0.0	5.088014402954182	0.0
47	0.0	0.0	0.0	5.900151782956942	0.0
48	0.0	0.0	0.0	6.7152459738092265	0.0
49	0.0	0.0	0.0	7.547095426142151	0.0
50	0.0	0.0	0.0	8.29648270922722	0.0
51	0.0	0.0	0.0	9.03765662884139	0.0
52	0.0	0.0	0.0	9.775545203067198	0.0
53	0.0	0.0	0.0	10.545630162098941	0.0
54	0.0	0.0	0.0	11.36368116380075	0.0
55	0.0	0.0	0.0	12.162677162250068	0.0
56	0.0	0.0	0.0	12.978099887641187	0.0
57	0.0	0.0	0.0	13.768882522619602	0.0
58	0.0	0.0	0.0	14.546195241505739	0.0
59	0.0	0.0	0.0	15.228890013207089	0.0
60	0.0	0.0	0.0	15.98484798706888	0.0
61	0.0	0.0	0.0	16.688568969255737	0.0
62	0.0	0.0	0.0	17.417258576394136	0.0
63	0.0	0.0	0.0	18.100938951711992	0.0
64	0.0	0.0	0.0	18.82962855885039	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTGAGA	20	0.0069471514	52.5	57
TGCACGG	50	1.7075357E-4	35.000004	47
ACGGTTA	50	1.7075357E-4	35.000004	50
ATTCGGG	40	0.002416649	35.0	45
CTTTGCG	40	0.002416649	35.0	38
CATCGTT	60	1.213615E-5	35.0	32
GGCGCTA	65	2.0989723E-5	32.307693	18
TGCTACC	55	2.9883228E-4	31.818184	38
ACCTTTG	55	2.9883228E-4	31.818184	42
TGCCGAG	55	2.9883228E-4	31.818184	57
CTCGTAA	45	0.0043035336	31.111113	29
ATTCGCA	45	0.0043035336	31.111113	6
CACTACG	45	0.0043035336	31.111113	4
CGTCCCG	45	0.0043035336	31.111113	35
GCCAACT	45	0.0043035336	31.111113	16
CTCAACG	80	2.4607434E-6	30.625	46
TAACCGG	80	2.4607434E-6	30.625	22
TCGTTGT	60	4.975788E-4	29.166666	34
CGGCTAC	85	3.946432E-6	28.82353	51
ACGTTGG	50	0.0072021727	28.000002	28
>>END_MODULE