FastQCFastQC Report
Wed 25 May 2016
SRR1781204_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781204_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences273842
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCGT30201.1028257170193032No Hit
TCTTATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCG3400.1241591866842924No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG4700.055.10638443
CTCGTAT4600.054.78260839
CGTCTTC4350.054.71264648
TACCTCG452.6933776E-854.44444736
TCGTATG4650.054.19354640
CGTATGC4750.053.7894741
CCGTCTT4500.053.66666847
ATCTCGT4050.053.58024637
TCTCGTA4050.053.58024638
GCCGTCT4600.053.26086846
CGTATTG200.00694641152.557
CCCAGAT200.00694641152.568
CGCGATC200.00694641152.526
GTATGCC4900.052.14285742
GTATCTC4200.051.66666435
TAACCGG352.0832716E-550.00000422
ATGCCGT5200.049.13461344
TGCCGTC5200.049.13461345
GTCTTCT5000.048.349
CTTGAAA5050.047.1287157
Produced by FastQC (version 0.11.5)