FastQCFastQC Report
Wed 25 May 2016
SRR1781203_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781203_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences91801
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATCA207.88988E-570.024
TTACTCG252.8202103E-670.019
ATCCGTA207.88988E-570.012
GAAGTCG150.002218371370.063
TCCGTAT207.88988E-570.013
GTATTAC252.379426E-455.99999616
CATCCGT252.379426E-455.99999611
GATACTT200.006931779452.57
GTTTCGG200.006931779452.521
TTTCGGG200.006931779452.522
GCTGCCA200.006931779452.548
TACGGAG200.006931779452.552
CTGCGGA200.006931779452.518
CTACGGA200.006931779452.551
GTATCAA200.006931779452.534
GGACAGT200.006931779452.56
TCGCATC305.8510236E-446.66666423
CGTATTA305.8510236E-446.66666415
CCGTATT305.8510236E-446.66666414
CTCGCAT305.8510236E-446.66666422
Produced by FastQC (version 0.11.5)