##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1781203_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	91801
Sequences flagged as poor quality	0
Sequence length	76
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.668881602596922	34.0	32.0	34.0	21.0	34.0
2	30.949608392065446	34.0	32.0	34.0	21.0	34.0
3	30.870241064912147	34.0	32.0	34.0	21.0	34.0
4	31.078702846374224	34.0	33.0	34.0	23.0	34.0
5	31.039422228516028	34.0	33.0	34.0	23.0	34.0
6	33.72343438524635	37.0	34.0	38.0	22.0	38.0
7	33.75857561464472	37.0	34.0	38.0	22.0	38.0
8	33.50413394189606	37.0	34.0	38.0	21.0	38.0
9	33.74962146381848	37.0	34.0	38.0	22.0	38.0
10-11	33.61523294953214	37.0	34.0	38.0	21.0	38.0
12-13	33.575565625646774	37.0	34.0	38.0	21.0	38.0
14-15	33.47402533741462	37.0	34.0	38.0	21.0	38.0
16-17	33.443540920033556	37.0	34.0	38.0	21.0	38.0
18-19	33.46692846483154	37.0	34.0	38.0	21.0	38.0
20-21	33.41533316630538	37.0	34.0	38.0	21.0	38.0
22-23	33.4903486890121	37.0	34.0	38.0	21.0	38.0
24-25	33.46169431705537	37.0	34.0	38.0	21.0	38.0
26-27	33.38839446193397	37.0	34.0	38.0	21.0	38.0
28-29	33.47690656964521	37.0	34.0	38.0	21.0	38.0
30-31	33.546894913998756	37.0	34.0	38.0	21.0	38.0
32-33	33.48611126240455	37.0	34.0	38.0	21.0	38.0
34-35	33.49822442021329	37.0	34.0	38.0	21.0	38.0
36-37	33.38168429537805	37.0	34.0	38.0	21.0	38.0
38-39	33.394957571268286	37.0	34.0	38.0	21.0	38.0
40-41	33.37295890022985	37.0	34.0	38.0	21.0	38.0
42-43	33.423383187546975	37.0	34.0	38.0	21.0	38.0
44-45	33.48823542227208	37.0	34.0	38.0	21.0	38.0
46-47	33.45540898247296	37.0	34.0	38.0	21.0	38.0
48-49	33.43399309375715	37.0	34.0	38.0	21.0	38.0
50-51	33.279740961427436	37.0	34.0	38.0	21.0	38.0
52-53	33.37764294506596	37.0	34.0	38.0	21.0	38.0
54-55	33.33986013224256	37.0	34.0	38.0	21.0	38.0
56-57	33.28825938715264	37.0	34.0	38.0	21.0	38.0
58-59	33.26280214812475	37.0	34.0	38.0	21.0	38.0
60-61	33.252638860143136	37.0	34.0	38.0	21.0	38.0
62-63	33.229469177895666	37.0	34.0	38.0	21.0	38.0
64-65	33.23436563871853	37.0	34.0	38.0	21.0	38.0
66-67	33.09227023670766	37.0	34.0	38.0	21.0	38.0
68-69	32.87793161294539	37.0	32.5	38.0	16.0	38.0
70-71	33.03358351216218	37.0	33.0	38.0	21.0	38.0
72-73	32.97839892811625	37.0	32.5	38.0	21.0	38.0
74-75	33.00560451411205	37.0	32.0	38.0	21.0	38.0
76	31.68134334048649	36.0	28.0	38.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	6.0
13	22.0
14	90.0
15	289.0
16	653.0
17	1080.0
18	1402.0
19	1394.0
20	1213.0
21	1036.0
22	1017.0
23	932.0
24	1161.0
25	1304.0
26	1481.0
27	1812.0
28	2039.0
29	2453.0
30	2895.0
31	3504.0
32	4182.0
33	5132.0
34	6454.0
35	8482.0
36	13197.0
37	28571.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.148734763237876	17.878890208167668	9.949782682105859	26.022592346488597
2	22.92894412914892	19.82984934804632	31.887452206402983	25.353754316401783
3	20.374505724338515	20.969270487249595	28.484439167329334	30.17178462108256
4	14.420322218712215	15.71551508153506	35.59547281620026	34.26868988355247
5	14.663238962538536	33.400507619742704	34.64232415768891	17.29392926002985
6	31.387457652966745	35.145586649382906	17.467129987690765	15.999825709959586
7	30.177231184845482	28.31123843966841	20.62831559569068	20.883214779795427
8	26.24263352251065	34.12707922571649	19.117438807856125	20.512848443916734
9	25.09776581954445	15.36148843694513	20.594546900360562	38.94619884314985
10-11	26.20069498153615	23.74266075532946	26.283482750732563	23.773161512401824
12-13	25.784577510048912	21.807496650363287	25.81398895436869	26.593936885219115
14-15	24.0471236696768	23.089617760155118	23.372294419450768	29.490964150717314
16-17	24.480125488829096	25.8412217731833	23.993202688423874	25.68545004956373
18-19	23.9283885796451	25.274779141839414	25.504624132634724	25.292208145880764
20-21	24.006274441454885	25.560723739392817	25.330334092221218	25.102667726931077
22-23	24.327077047090988	24.861929608609927	25.758978660363173	25.0520146839359
24-25	24.45833923377741	24.90168952407926	24.9267437173887	25.713227524754632
26-27	23.450724937636842	25.31617302643762	25.693619895208116	25.539482140717425
28-29	23.750830600973845	25.044934151044107	26.306358318536837	24.89787692944521
30-31	24.146251130161982	24.975762791254997	25.837409178549255	25.040576900033766
32-33	23.82326989902071	25.36192416204617	25.67510157841418	25.139704360518948
34-35	24.43328504046797	24.59940523523709	25.857561464472063	25.10974825982288
36-37	24.182743107373557	24.674023158789122	25.530767638696744	25.612466095140572
38-39	23.829805775536215	24.848313199202625	26.44361172536247	24.878269299898694
40-41	23.894075227938693	24.154420975806364	27.101011971547152	24.85049182470779
42-43	23.63046154181327	24.243199965141994	26.607553294626417	25.51878519841832
44-45	22.654981971873944	24.446356793498982	27.298177579764925	25.60048365486215
46-47	23.178396749490744	24.33252361085391	27.17835317698064	25.310726462674698
48-49	22.930578098277795	24.659862093005522	27.01495626409298	25.394603544623696
50-51	22.529166348950447	25.04112155641006	26.843389505560943	25.586322589078552
52-53	22.572738859053825	24.565636539906972	27.348285966383806	25.513338634655398
54-55	22.021001949869827	24.905502118713304	27.221381030707725	25.852114900709143
56-57	22.163157264082088	24.742105205825645	27.126610821232884	25.96812670885938
58-59	22.196925959412205	24.725220858160586	27.20830927767671	25.869543904750493
60-61	22.155532074813998	24.59722660973192	26.990446727159835	26.256794588294248
62-63	21.891918388688577	24.314005294059978	27.46102983627629	26.333046480975153
64-65	22.48831712072853	24.571627760046187	26.750253265214972	26.1898018540103
66-67	22.454548425398414	24.792758248820817	26.18544460299997	26.567248722780796
68-69	22.0858160586486	24.966503632858032	26.176185444603	26.77149486389037
70-71	22.152264136556248	25.00190629731702	26.422914783063366	26.422914783063366
72-73	22.224158778226816	24.242110652389407	26.606463981873834	26.92726658750994
74-75	22.036252328406007	24.699077352098563	26.766048300127448	26.49862201936798
76	22.1206740667313	25.002995610069608	26.219757954706374	26.65657236849272
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.5
12	1.0
13	3.0
14	4.5
15	8.5
16	27.5
17	42.0
18	58.5
19	93.0
20	125.5
21	140.0
22	184.0
23	246.5
24	307.0
25	349.0
26	438.5
27	578.0
28	718.5
29	809.0
30	932.0
31	1130.5
32	1384.5
33	1563.0
34	1784.0
35	2192.0
36	2519.0
37	2659.0
38	2926.0
39	3435.5
40	4004.5
41	4444.5
42	4558.0
43	4650.0
44	4708.5
45	4684.0
46	4693.0
47	4595.0
48	4339.0
49	4057.5
50	3934.0
51	3653.5
52	3104.0
53	2746.0
54	2657.0
55	2548.0
56	2280.5
57	1984.0
58	1846.0
59	1739.5
60	1628.5
61	1544.5
62	1465.0
63	1420.5
64	1363.5
65	1307.5
66	1226.0
67	1188.0
68	1205.5
69	1224.5
70	1255.0
71	1284.0
72	1196.0
73	1087.5
74	1016.0
75	965.0
76	882.5
77	742.0
78	631.0
79	578.0
80	500.0
81	349.0
82	222.0
83	168.0
84	154.5
85	103.5
86	49.5
87	33.0
88	26.0
89	14.5
90	6.0
91	2.5
92	3.0
93	2.5
94	2.5
95	1.5
96	0.0
97	0.0
98	0.5
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	91801.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.907800568621255
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.34151496556896	46.870949118201324
2	14.605918047317108	18.668641953791354
3	6.033953773777869	11.56850143244627
4	2.6539169564328082	6.784239823095609
5	1.1761096338719574	3.758128996416161
6	0.6391900184086725	2.4509536933148874
7	0.44487625281243603	1.9901743989716887
8	0.28635712824708526	1.4640363394734264
9	0.20965432603804457	1.2058692171109247
>10	0.6085088975250562	5.238505027178353
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0010893127525843946	0.0
2	0.0	0.0	0.0	0.0010893127525843946	0.0
3	0.0	0.0	0.0	0.0010893127525843946	0.0
4	0.0	0.0	0.0	0.005446563762921973	0.0
5	0.0	0.0	0.0	0.006535876515506367	0.0
6	0.0	0.0	0.0	0.008714502020675157	0.0
7	0.0	0.0	0.0	0.008714502020675157	0.0
8	0.0	0.0	0.0	0.008714502020675157	0.0
9	0.0	0.0	0.0	0.008714502020675157	0.0
10	0.0	0.0	0.0	0.010893127525843945	0.0
11	0.0	0.0	0.0	0.010893127525843945	0.0
12	0.0	0.0	0.0	0.010893127525843945	0.0
13	0.0	0.0	0.0	0.01198244027842834	0.0
14	0.0	0.0	0.0	0.01198244027842834	0.0
15	0.0	0.0	0.0	0.01198244027842834	0.0
16	0.0	0.0	0.0	0.01198244027842834	0.0
17	0.0	0.0	0.0	0.01198244027842834	0.0
18	0.0	0.0	0.0	0.01198244027842834	0.0
19	0.0	0.0	0.0	0.01198244027842834	0.0
20	0.0	0.0	0.0	0.013071753031012734	0.0
21	0.0	0.0	0.0	0.013071753031012734	0.0
22	0.0	0.0	0.0	0.022875567804272284	0.0
23	0.0	0.0	0.0	0.025054193309441072	0.0
24	0.0	0.0	0.0	0.030500757072363046	0.0
25	0.0	0.0	0.0	0.03159006982494744	0.0
26	0.0	0.0	0.0	0.032679382577531835	0.0
27	0.0	0.0	0.0	0.04357251010337578	0.0
28	0.0	0.0	0.0	0.07843051818607641	0.0
29	0.0	0.0	0.0	0.10130608599034868	0.0
30	0.0	0.0	0.0	0.12527096654720538	0.0
31	0.0	0.0	0.0	0.14487859609372447	0.0
32	0.0	0.0	0.0	0.1764686659186719	0.0
33	0.0	0.0	0.0	0.23638086731081362	0.0
34	0.0	0.0	0.0	0.3191686365072276	0.0
35	0.0	0.0	0.0	0.46295791984836765	0.0
36	0.0	0.0	0.0	0.6503197132928835	0.0
37	0.0	0.0	0.0	0.9509700330061764	0.0
38	0.0	0.0	0.0	1.2832104225444168	0.0
39	0.0	0.0	0.0	1.6078256228145662	0.0
40	0.0	0.0	0.0	1.8899576257339246	0.0
41	0.0	0.0	0.0	2.1971438219627237	0.0
42	0.0	0.0	0.0	2.5468132155423144	0.0
43	0.0	0.0	0.0	2.9553054977614623	0.0
44	0.0	0.0	0.0	3.3910305987952203	0.0
45	0.0	0.0	0.0	3.8953824032417947	0.0
46	0.0	0.0	0.0	4.462914347338264	0.0
47	0.0	0.0	0.0	5.065304299517434	0.0
48	0.0	0.0	0.0	5.553316412675243	0.0
49	0.0	0.0	0.0	6.051132340606311	0.0
50	0.0	0.0	0.0	6.559841396063224	0.0
51	0.0	0.0	0.0	7.027156566921929	0.0
52	0.0	0.0	0.0	7.481399984749621	0.0
53	0.0	0.0	0.0	7.941089966340236	0.0
54	0.0	0.0	0.0	8.43999520702389	0.0
55	0.0	0.0	0.0	8.910578316140347	0.0
56	0.0	0.0	0.0	9.461770568948051	0.0
57	0.0	0.0	0.0	9.975926188167884	0.0
58	0.0	0.0	0.0	10.41818716571715	0.0
59	0.0	0.0	0.0	10.907288591627543	0.0
60	0.0	0.0	0.0	11.356085445692313	0.0
61	0.0	0.0	0.0	11.812507489025174	0.0
62	0.0	0.0	0.0	12.234071524275334	0.0
63	0.0	0.0	0.0	12.659992810535833	0.0
64	0.0	0.0	0.0	13.093539286064422	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCATCA	20	7.88988E-5	70.0	24
TTACTCG	25	2.8202103E-6	70.0	19
ATCCGTA	20	7.88988E-5	70.0	12
GAAGTCG	15	0.0022183713	70.0	63
TCCGTAT	20	7.88988E-5	70.0	13
GTATTAC	25	2.379426E-4	55.999996	16
CATCCGT	25	2.379426E-4	55.999996	11
GATACTT	20	0.0069317794	52.5	7
GTTTCGG	20	0.0069317794	52.5	21
TTTCGGG	20	0.0069317794	52.5	22
GCTGCCA	20	0.0069317794	52.5	48
TACGGAG	20	0.0069317794	52.5	52
CTGCGGA	20	0.0069317794	52.5	18
CTACGGA	20	0.0069317794	52.5	51
GTATCAA	20	0.0069317794	52.5	34
GGACAGT	20	0.0069317794	52.5	6
TCGCATC	30	5.8510236E-4	46.666664	23
CGTATTA	30	5.8510236E-4	46.666664	15
CCGTATT	30	5.8510236E-4	46.666664	14
CTCGCAT	30	5.8510236E-4	46.666664	22
>>END_MODULE