FastQCFastQC Report
Wed 25 May 2016
SRR1781203_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781203_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences83353
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCGT36704.402960901227311No Hit
TCTTATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCG4010.4810864635945916No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCC2780.3335212889758017No Hit
CTTATACACATCTCCGAGCCCACGAGACTCTTATTAACCTCGTATGCCGT1840.22074790349477522No Hit
CTTATACACATCTCCGAGCCCACGAGACTCTTATTAAACTCGTATGCCGT1130.13556800595059565No Hit
CTTATACACATCTCCGAGCCCACGAGACTCTTATTAAGCTCGTATGCCGT850.1019759336796516No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTAAGC207.885622E-570.033
GCTCGTA252.378149E-456.00000438
TAAGCTC252.378149E-456.00000435
CGTATGC5550.053.60360341
TCGTATG5550.053.60360340
TATGCCG5500.053.45454843
CCGTCTT5600.053.12547
CTCGTAT5550.052.97297339
CGGGTGG200.006929550352.534
TCTAACT200.006929550352.541
AATCTCG5000.052.536
CCAGTTT200.006929550352.59
ATGCCGT5600.052.544
CTCTAAA200.006929550352.561
CGTCTTC5650.052.03539748
TGCCGTC5650.052.03539745
TCTCGTA5050.051.98019838
GCCGTCT5700.051.57894546
GTATGCC5800.051.29310642
CTTGAAA5550.051.08107857