##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1781203_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	83353
Sequences flagged as poor quality	0
Sequence length	76
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.22410711072187	34.0	34.0	34.0	32.0	34.0
2	33.27502309454969	34.0	34.0	34.0	33.0	34.0
3	33.33728839993761	34.0	34.0	34.0	33.0	34.0
4	33.40837162429666	34.0	34.0	34.0	34.0	34.0
5	33.422228354108434	34.0	34.0	34.0	34.0	34.0
6	36.8251652609984	38.0	37.0	38.0	34.0	38.0
7	36.70587741293055	38.0	37.0	38.0	34.0	38.0
8	36.46184300505081	38.0	37.0	38.0	34.0	38.0
9	36.447818314877686	38.0	37.0	38.0	34.0	38.0
10-11	36.385553009489755	38.0	37.0	38.0	34.0	38.0
12-13	36.3274687173827	38.0	37.0	38.0	34.0	38.0
14-15	36.33424711768023	38.0	37.0	38.0	34.0	38.0
16-17	36.222307535421635	38.0	37.0	38.0	33.5	38.0
18-19	36.19575180257459	38.0	37.0	38.0	33.5	38.0
20-21	36.16614279030149	38.0	37.0	38.0	33.0	38.0
22-23	36.24301464854294	38.0	37.0	38.0	34.0	38.0
24-25	36.19398821878037	38.0	37.0	38.0	33.5	38.0
26-27	35.924987702902115	38.0	36.5	38.0	31.5	38.0
28-29	35.87195421880436	38.0	36.0	38.0	31.0	38.0
30-31	35.95200532674289	38.0	37.0	38.0	32.0	38.0
32-33	35.923626024258276	38.0	36.5	38.0	32.0	38.0
34-35	35.9445130949096	38.0	37.0	38.0	32.0	38.0
36-37	35.86276438760453	38.0	36.0	38.0	31.5	38.0
38-39	35.47583170371792	38.0	36.0	38.0	31.0	38.0
40-41	35.75005098796684	38.0	36.0	38.0	31.0	38.0
42-43	35.870196633594475	38.0	36.0	38.0	31.5	38.0
44-45	35.863532206399285	38.0	36.0	38.0	31.0	38.0
46-47	35.866729451849366	38.0	36.0	38.0	31.5	38.0
48-49	35.829256295514256	38.0	36.0	38.0	31.5	38.0
50-51	35.712601825969074	38.0	36.0	38.0	31.0	38.0
52-53	35.73054959029669	38.0	36.0	38.0	31.0	38.0
54-55	35.74698571137212	38.0	36.0	38.0	31.0	38.0
56-57	35.69672357323671	38.0	36.0	38.0	31.0	38.0
58-59	35.62713999496119	38.0	36.0	38.0	31.0	38.0
60-61	35.60240183316738	38.0	36.0	38.0	31.0	38.0
62-63	35.58430410423141	38.0	36.0	38.0	31.0	38.0
64-65	35.53832495531054	38.0	36.0	38.0	31.0	38.0
66-67	35.454590716590886	38.0	36.0	38.0	31.0	38.0
68-69	35.22934387484554	38.0	36.0	38.0	28.5	38.0
70-71	34.794758437008866	38.0	35.5	38.0	26.0	38.0
72-73	34.45634830180077	38.0	35.0	38.0	24.0	38.0
74-75	34.48731899271772	38.0	35.0	38.0	24.0	38.0
76	33.60414142262426	37.0	34.0	38.0	23.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	1.0
21	3.0
22	12.0
23	35.0
24	79.0
25	177.0
26	315.0
27	543.0
28	861.0
29	1362.0
30	2020.0
31	2931.0
32	3953.0
33	5321.0
34	7170.0
35	10083.0
36	13686.0
37	34800.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.16221371756265	17.159550346118316	8.823917555456912	30.854318380862118
2	21.918827156791	18.449245977949204	35.002939306323704	24.628987558936092
3	20.2704161817811	20.25242042877881	31.851283097189064	27.625880292251026
4	14.067879980324644	20.054467145753602	33.43011049392343	32.447542379998325
5	14.211846004342977	31.8656796995909	37.87626120235624	16.046213093709884
6	30.640768778568255	39.027989394502896	15.986227250368913	14.345014576559931
7	28.267728815999426	26.9696351661008	19.958489796408045	24.804146221491727
8	24.711768022746632	37.90025553969264	17.106762803978263	20.281213633582475
9	23.459263613787147	14.270632130817127	18.842753110265978	43.42735114512975
10-11	24.934315501541636	25.31282617302317	27.175386608760334	22.577471716674864
12-13	24.804146221491727	20.575144265953234	26.868858949287965	27.751850563267066
14-15	22.851007162309696	21.48632922630259	21.707077129797366	33.955586481590345
16-17	26.31758904898444	27.27016424123907	22.147373219920098	24.264873489856395
18-19	26.393171211594062	24.42803498374384	22.98417573452665	26.19461807013545
20-21	23.100548270608137	24.106510863436228	22.857605605077204	29.935335260878432
22-23	22.957781963456625	26.853862488452723	23.4934555444915	26.694900003599148
24-25	26.472352524804144	26.599522512687006	22.915791873117943	24.012333089390903
26-27	25.667342507168307	27.291759144841816	23.13294062601226	23.907957721977613
28-29	23.528247333629263	23.82817655033412	26.896452437224816	25.7471236788118
30-31	23.216920806689618	23.674612791381232	26.622916991589985	26.48554941033916
32-33	22.516286156467075	26.408167672429308	27.254567921970413	23.820978249133205
34-35	23.19412618622005	23.72500089978765	29.956930164481182	23.123942749511116
36-37	23.15813468021547	29.10093218000552	24.032728276126832	23.708204863652178
38-39	22.873801782779264	24.65538133000612	26.5803270428179	25.890489844396726
40-41	22.708840713591595	23.012369080896907	27.77464518373664	26.50414502177486
42-43	25.64994661259943	23.100548270608137	26.985231485369454	24.264273631422984
44-45	22.31233428910777	26.140630811128574	27.874821542116063	23.672213357647596
46-47	24.985303468381463	23.24691372836011	25.309826880856118	26.457955922402316
48-49	24.848535745564046	22.86480390627812	25.09747699542908	27.189183352728758
50-51	21.728672033400116	23.242714719326237	28.01818770770098	27.010425539572662
52-53	21.23918755173779	22.825813108106487	31.43498134440272	24.500017995753
54-55	20.98364785910525	23.420872674048923	28.578455484505653	27.017023982340167
56-57	24.06212133936391	23.1971254783871	25.379410459131645	27.361342723117343
58-59	21.307571413146498	23.143738077813637	30.67915971830648	24.86953079073339
60-61	24.248077453720924	26.083044401521242	25.041090302688566	24.62778784206927
62-63	20.351996928724823	29.88494715247202	24.657780763739755	25.105275155063406
64-65	20.71251184720406	29.93773469461207	24.360850839201948	24.98890261898192
66-67	20.700514678535868	30.041510203591955	23.924753758113084	25.333221359759094
68-69	20.64892685326263	30.499202188283565	23.666814631746906	25.185056326706896
70-71	21.05143186208055	28.27492711720034	24.63138699266973	26.042254028049385
72-73	21.454536729331878	25.82330569985483	25.650546471032836	27.07161109978045
74-75	20.950655645267716	25.970870874473622	26.114237040058548	26.96423644020011
76	20.86427603085672	25.918683190766977	26.25460391347642	26.962436864899885
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.5
10	0.5
11	0.0
12	0.0
13	0.5
14	1.5
15	6.5
16	21.0
17	31.0
18	48.0
19	72.5
20	86.0
21	92.0
22	128.0
23	209.5
24	291.5
25	328.0
26	393.5
27	498.5
28	574.5
29	611.0
30	697.5
31	928.5
32	1224.0
33	1375.0
34	1505.0
35	1773.0
36	2352.5
37	2794.0
38	3242.0
39	4234.5
40	4822.5
41	4647.5
42	4429.0
43	4405.0
44	4184.5
45	3873.5
46	3759.0
47	3762.0
48	3619.0
49	3337.0
50	3201.0
51	2974.0
52	2529.5
53	2280.5
54	2249.0
55	2167.0
56	1971.0
57	1737.5
58	1618.0
59	1507.0
60	1365.0
61	1302.0
62	1270.0
63	1190.5
64	1139.0
65	1136.5
66	1157.5
67	1209.0
68	1210.0
69	1163.0
70	1087.0
71	1059.0
72	1042.0
73	1014.0
74	961.5
75	920.0
76	855.5
77	710.5
78	561.5
79	493.0
80	441.0
81	326.0
82	231.5
83	200.0
84	164.5
85	98.5
86	48.5
87	29.0
88	21.0
89	10.5
90	6.0
91	2.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	83353.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.13546003143258
#Duplication Level	Percentage of deduplicated	Percentage of total
1	62.783924136373905	33.36052691564791
2	20.905396251975617	22.216356939762218
3	8.36531948521111	13.334852974697972
4	3.370964100248363	7.164709128645639
5	1.6459697448634003	4.372967979556824
6	0.8918491758862045	2.8433289743620507
7	0.5125310453827049	1.9063501013760753
8	0.4018965906525175	1.7083968183508693
9	0.2799729058478212	1.3388840233704846
>10	0.8241137954391511	5.919403020887071
>50	0.006773538044705351	0.2603385600998165
>100	0.009031384059607135	1.1709236620157644
>500	0.0	0.0
>1k	0.002257846014901784	4.402960901227311
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCGT	3670	4.402960901227311	No Hit
TCTTATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCG	401	0.4810864635945916	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCC	278	0.3335212889758017	No Hit
CTTATACACATCTCCGAGCCCACGAGACTCTTATTAACCTCGTATGCCGT	184	0.22074790349477522	No Hit
CTTATACACATCTCCGAGCCCACGAGACTCTTATTAAACTCGTATGCCGT	113	0.13556800595059565	No Hit
CTTATACACATCTCCGAGCCCACGAGACTCTTATTAAGCTCGTATGCCGT	85	0.1019759336796516	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0011997168668194307	0.0
2	0.0	0.0	0.0	0.0011997168668194307	0.0
3	0.0	0.0	0.0	0.0011997168668194307	0.0
4	0.0	0.0	0.0	0.004798867467277723	0.0
5	0.0	0.0	0.0	0.005998584334097153	0.0
6	0.0	0.0	0.0	0.008398018067736015	0.0
7	0.0	0.0	0.0	0.008398018067736015	0.0
8	0.0	0.0	0.0	0.008398018067736015	0.0
9	0.0	0.0	0.0	0.008398018067736015	0.0
10	0.0	0.0	0.0	0.010797451801374876	0.0
11	0.0	0.0	0.0	0.010797451801374876	0.0
12	0.0	0.0	0.0	0.010797451801374876	0.0
13	0.0	0.0	0.0	0.011997168668194306	0.0
14	0.0	0.0	0.0	0.011997168668194306	0.0
15	0.0	0.0	0.0	0.011997168668194306	0.0
16	0.0	0.0	0.0	0.011997168668194306	0.0
17	0.0	0.0	0.0	0.011997168668194306	0.0
18	0.0	0.0	0.0	0.011997168668194306	0.0
19	0.0	0.0	0.0	0.011997168668194306	0.0
20	0.0	0.0	0.0	0.011997168668194306	0.0
21	0.0	0.0	0.0	0.011997168668194306	0.0
22	0.0	0.0	0.0	0.011997168668194306	0.0
23	0.0	0.0	0.0	0.013196885535013737	0.0
24	0.0	0.0	0.0	0.013196885535013737	0.0
25	0.0	0.0	0.0	0.014396602401833167	0.0
26	0.0	0.0	0.0	0.015596319268652598	0.0
27	0.0	0.0	0.0	0.031192638537305195	0.0
28	0.0	0.0	0.0	0.06838386140870754	0.0
29	0.0	0.0	0.0	0.09237819874509616	0.0
30	0.0	0.0	0.0	0.12237112041558192	0.0
31	0.0	0.0	0.0	0.14396602401833167	0.0
32	0.0	0.0	0.0	0.17755809628927574	0.0
33	0.0	0.0	0.0	0.24234280709752498	0.0
34	0.0	0.0	0.0	0.328722421508524	0.0
35	0.0	0.0	0.0	0.4726884455268557	0.0
36	0.0	0.0	0.0	0.6670425779516034	0.0
37	0.0	0.0	0.0	1.0017635837942245	0.0
38	0.0	0.0	0.0	1.3364845896368458	0.0
39	0.0	0.0	0.0	1.6748047460799251	0.0
40	0.0	0.0	0.0	1.9711348121843244	0.0
41	0.0	0.0	0.0	2.284260914424196	0.0
42	0.0	0.0	0.0	2.6621717274723165	0.0
43	0.0	0.0	0.0	3.095269516394131	0.0
44	0.0	0.0	0.0	3.552361642652334	0.0
45	0.0	0.0	0.0	4.079037347186064	0.0
46	0.0	0.0	0.0	4.652502009525752	0.0
47	0.0	0.0	0.0	5.2631578947368425	0.0
48	0.0	0.0	0.0	5.7766367137355585	0.0
49	0.0	0.0	0.0	6.280517797799719	0.0
50	0.0	0.0	0.0	6.813192086667547	0.0
51	0.0	0.0	0.0	7.305076002063513	0.0
52	0.0	0.0	0.0	7.782563315057646	0.0
53	0.0	0.0	0.0	8.252852326850864	0.0
54	0.0	0.0	0.0	8.771130013316856	0.0
55	0.0	0.0	0.0	9.240219308243255	0.0
56	0.0	0.0	0.0	9.793288783847013	0.0
57	0.0	0.0	0.0	10.363154295586241	0.0
58	0.0	0.0	0.0	10.838242174846735	0.0
59	0.0	0.0	0.0	11.33492495770998	0.0
60	0.0	0.0	0.0	11.79681595143546	0.0
61	0.0	0.0	0.0	12.252708360826844	0.0
62	0.0	0.0	0.0	12.693004450949577	0.0
63	0.0	0.0	0.0	13.1452977097405	0.0
64	0.0	0.0	0.0	13.598790685398246	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTAAGC	20	7.885622E-5	70.0	33
GCTCGTA	25	2.378149E-4	56.000004	38
TAAGCTC	25	2.378149E-4	56.000004	35
CGTATGC	555	0.0	53.603603	41
TCGTATG	555	0.0	53.603603	40
TATGCCG	550	0.0	53.454548	43
CCGTCTT	560	0.0	53.125	47
CTCGTAT	555	0.0	52.972973	39
CGGGTGG	20	0.0069295503	52.5	34
TCTAACT	20	0.0069295503	52.5	41
AATCTCG	500	0.0	52.5	36
CCAGTTT	20	0.0069295503	52.5	9
ATGCCGT	560	0.0	52.5	44
CTCTAAA	20	0.0069295503	52.5	61
CGTCTTC	565	0.0	52.035397	48
TGCCGTC	565	0.0	52.035397	45
TCTCGTA	505	0.0	51.980198	38
GCCGTCT	570	0.0	51.578945	46
GTATGCC	580	0.0	51.293106	42
CTTGAAA	555	0.0	51.081078	57
>>END_MODULE