FastQCFastQC Report
Wed 25 May 2016
SRR1781202_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781202_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences217617
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGGTC200.006944503652.560
CTTGCGT350.001253527140.049
CGATAGA350.001253527140.037
TACGTAC459.177328E-538.88888510
CGTCCCA556.6742195E-638.18181650
CGTGCGA400.002415140435.010
CTACGTA501.7059395E-435.09
CGTAGCG400.002415140435.015
TTGCGTG400.002415140435.050
ATGCGAT400.002415140435.026
TCGTCCC652.0962394E-532.30769349
GATGTAG552.985543E-431.818181
CGGAATC450.004300863531.11110920
TTGGCAT450.004300863531.11110932
TGTTCGA450.004300863531.11110937
CGAATTC450.004300863531.11110941
ATTGCAC450.004300863531.11110932
CCGCAAG450.004300863531.11110911
TGCGTGA450.004300863531.11110951
CCCTAGT604.971183E-429.1666661