##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1781202_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	217617
Sequences flagged as poor quality	0
Sequence length	76
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.29560650133032	34.0	32.0	34.0	23.0	34.0
2	31.379602696480514	34.0	33.0	34.0	23.0	34.0
3	31.364277606988423	34.0	33.0	34.0	23.0	34.0
4	31.525018725559125	34.0	33.0	34.0	23.0	34.0
5	31.467316432080214	34.0	33.0	34.0	23.0	34.0
6	34.34086950927547	37.0	34.0	38.0	25.0	38.0
7	34.17950343952908	37.0	34.0	38.0	24.0	38.0
8	34.008928530399736	37.0	34.0	38.0	24.0	38.0
9	34.33926577427315	37.0	34.0	38.0	24.0	38.0
10-11	34.18905232587528	37.0	34.0	38.0	24.0	38.0
12-13	34.17316891603138	37.0	34.0	38.0	24.0	38.0
14-15	34.043229619009544	37.0	34.0	38.0	23.5	38.0
16-17	34.026238758920485	37.0	34.0	38.0	23.5	38.0
18-19	34.07932744224946	37.0	34.0	38.0	23.5	38.0
20-21	34.017634192181674	37.0	34.0	38.0	23.5	38.0
22-23	34.0768276375467	37.0	34.0	38.0	23.5	38.0
24-25	34.071828028141184	37.0	34.0	38.0	23.5	38.0
26-27	33.98574789653382	37.0	34.0	38.0	23.0	38.0
28-29	34.0597195991122	37.0	34.0	38.0	23.5	38.0
30-31	34.15309465712697	37.0	34.0	38.0	24.0	38.0
32-33	34.09618274307614	37.0	34.0	38.0	23.5	38.0
34-35	34.13351438536512	37.0	34.0	38.0	24.0	38.0
36-37	33.98260016450921	37.0	34.0	38.0	23.5	38.0
38-39	33.99086698189939	37.0	34.0	38.0	23.0	38.0
40-41	33.98052082328127	37.0	34.0	38.0	23.5	38.0
42-43	34.052387910870934	37.0	34.0	38.0	24.0	38.0
44-45	34.085282859335436	37.0	34.0	38.0	24.0	38.0
46-47	34.06629307453002	37.0	34.0	38.0	24.0	38.0
48-49	34.05679473570539	37.0	34.0	38.0	24.0	38.0
50-51	33.9317332745144	37.0	34.0	38.0	23.0	38.0
52-53	34.0001401544916	37.0	34.0	38.0	23.5	38.0
54-55	33.961990561399155	37.0	34.0	38.0	23.0	38.0
56-57	33.93350473538372	37.0	34.0	38.0	23.5	38.0
58-59	33.95154101012329	37.0	34.0	38.0	23.0	38.0
60-61	33.915041104325496	37.0	34.0	38.0	23.0	38.0
62-63	33.90008822840127	37.0	34.0	38.0	23.0	38.0
64-65	33.9153765560595	37.0	34.0	38.0	23.0	38.0
66-67	33.82549616987643	37.0	34.0	38.0	23.0	38.0
68-69	33.58715541524789	37.0	34.0	38.0	22.5	38.0
70-71	33.74747836795838	37.0	34.0	38.0	23.0	38.0
72-73	33.703164274849854	37.0	34.0	38.0	23.0	38.0
74-75	33.698911390194695	37.0	34.0	38.0	23.0	38.0
76	32.39061286572281	37.0	31.0	38.0	21.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	4.0
13	24.0
14	108.0
15	286.0
16	673.0
17	1205.0
18	1595.0
19	1778.0
20	1771.0
21	1831.0
22	1979.0
23	2212.0
24	2665.0
25	3095.0
26	3676.0
27	4345.0
28	5182.0
29	6107.0
30	7145.0
31	8478.0
32	10199.0
33	12677.0
34	16056.0
35	21302.0
36	32125.0
37	71097.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.10898964694854	19.112936948859694	9.368293837338076	25.40977956685369
2	22.780389399725205	20.141349251207398	32.47494451260701	24.603316836460387
3	19.748916674708315	22.195876241286296	29.594654829356166	28.460552254649222
4	14.014530114834777	16.246892476231174	37.68179875653097	32.056778652403075
5	13.503540624124035	34.37093609414706	36.308744261707496	15.816779020021412
6	30.54462907139181	37.46829277259025	17.570399235350344	14.416678920667596
7	28.919615655026952	29.14156522698135	22.1081073629358	19.8307117550559
8	25.673545724828482	34.777154358345165	19.292150888947095	20.257149027879258
9	24.726928502828365	14.875676073100907	21.38435875873668	39.01303666533405
10-11	25.7036444763047	24.296355523695297	27.916477113460804	22.083522886539196
12-13	25.171804527689766	22.80066079548196	26.96394804621892	25.063586630609354
14-15	23.553361073264206	24.027589819705767	24.056769592379254	28.362279514650773
16-17	23.949415716603024	26.986862239622823	25.164624087272593	23.89909795650156
18-19	22.984647339132515	26.763993621821825	26.60591773620627	23.645441302839394
20-21	23.4021239149515	26.611202249824235	26.381440788173716	23.605233047050554
22-23	23.64917182290865	26.006300073753597	26.827699186412797	23.516828916924958
24-25	23.150764875905836	26.37799436624896	26.674616413239775	23.79662434460543
26-27	22.582794542705763	26.463235868521302	27.079685870129634	23.874283718643305
28-29	23.34123712761411	26.3508825137742	27.308758047395195	22.999122311216492
30-31	23.55537480987239	26.193725674005247	26.742625805888327	23.508273710234036
32-33	22.802446500043654	26.6633581016189	26.67668426639463	23.857511131942815
34-35	23.71138284233309	25.78475027226733	27.124948878074782	23.378918007324796
36-37	23.923222909974864	25.942596396421237	26.385346733021777	23.748833960582125
38-39	23.01934131984174	26.615108194672292	27.166076179710224	23.199474305775745
40-41	23.179255297150497	25.519605545522637	27.711070366745243	23.59006879058162
42-43	23.054265062012618	25.904455993787252	27.22627368266266	23.815005261537472
44-45	22.39416038269069	25.80634784966248	27.562414701057364	24.237077066589467
46-47	22.785903674804818	25.514550793366325	27.55368376551464	24.145861766314212
48-49	22.17956317750911	25.670099302903726	28.139805254185106	24.01053226540206
50-51	22.296562990398247	25.62604398104004	27.768115009661493	24.309278018900223
52-53	22.00999921881103	25.40403553031243	28.381973834764747	24.20399141611179
54-55	21.633879706089136	25.91203812202172	28.42746660417155	24.026615567717595
56-57	21.38997132563782	25.946162046908317	28.283076612013826	24.38079001544004
58-59	21.776202117491362	25.610708771413865	28.030108080288212	24.582981030806557
60-61	21.62892434379825	25.418397544298216	28.269290860966105	24.68338725093743
62-63	21.05888353797515	25.346711638850085	28.3912947577384	25.203110065436363
64-65	21.65236012057937	25.52179067715609	27.90442798323653	24.921421219028012
66-67	21.565509888978752	25.31086868612602	27.677192853466657	25.44642857142857
68-69	21.318285420189692	25.579001544004115	27.213532093228437	25.88918094257775
70-71	21.814112932872582	25.484109624292334	27.381948018528053	25.319829424307038
72-73	21.883960738181017	25.020448864054114	27.126222336592896	25.96936806117197
74-75	21.44051907947945	25.47698698625101	27.185960591133007	25.896533343136536
76	21.69050069847805	25.629089772810822	26.941952797588414	25.73845673112271
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.5
10	1.0
11	1.0
12	1.0
13	1.0
14	5.0
15	17.0
16	27.5
17	30.0
18	46.5
19	103.5
20	166.5
21	189.0
22	229.0
23	343.5
24	527.5
25	637.0
26	801.0
27	1086.5
28	1472.0
29	1736.0
30	2009.5
31	2545.5
32	3264.0
33	3720.0
34	4180.5
35	5152.0
36	6504.5
37	7346.0
38	7951.5
39	9476.0
40	11099.0
41	12452.5
42	13102.0
43	13236.0
44	13536.5
45	13729.5
46	13756.0
47	13368.5
48	12275.5
49	11180.0
50	10790.0
51	9837.5
52	8094.0
53	6886.5
54	6470.0
55	6069.5
56	5163.5
57	4263.5
58	3869.0
59	3449.5
60	2840.0
61	2476.0
62	2302.0
63	2179.0
64	1956.0
65	1837.0
66	1814.5
67	1811.0
68	1772.5
69	1644.0
70	1580.0
71	1606.0
72	1540.0
73	1394.5
74	1246.5
75	1178.0
76	1109.0
77	947.0
78	773.0
79	692.0
80	595.5
81	429.0
82	328.5
83	298.0
84	233.0
85	144.5
86	103.0
87	85.0
88	55.5
89	24.5
90	16.5
91	6.0
92	2.0
93	3.0
94	4.0
95	2.5
96	1.0
97	1.0
98	3.0
99	3.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	4.595229233010289E-4
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	2.2976146165051446E-4
14-15	2.2976146165051446E-4
16-17	0.0
18-19	0.0
20-21	0.0
22-23	2.2976146165051446E-4
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	2.2976146165051446E-4
52-53	0.0
54-55	0.0
56-57	4.595229233010289E-4
58-59	4.595229233010289E-4
60-61	4.595229233010289E-4
62-63	4.595229233010289E-4
64-65	4.595229233010289E-4
66-67	4.595229233010289E-4
68-69	4.595229233010289E-4
70-71	4.595229233010289E-4
72-73	4.595229233010289E-4
74-75	4.595229233010289E-4
76	4.595229233010289E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	217617.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.31649569353007
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.40218698082916	34.015869640743304
2	14.825695186822072	14.326512752953564
3	5.773312267759226	8.368386519677792
4	2.3187235807084634	4.481303916071484
5	1.3176975835060585	3.183326480942273
6	0.8897439758429609	2.579358658629645
7	0.6383789438627923	2.159096344439081
8	0.5117003787163174	1.9778855315699724
9	0.4378878219084329	1.9041484555339259
>10	2.810860754909126	24.76308658178044
>50	0.07281505857951188	2.189457442908103
>100	9.974665558837243E-4	0.051567674750421975
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	9.190458466020577E-4	0.0
2	0.0	0.0	0.0	9.190458466020577E-4	0.0
3	0.0	0.0	0.0	9.190458466020577E-4	0.0
4	0.0	0.0	0.0	0.0013785687699030865	0.0
5	0.0	0.0	0.0	0.0013785687699030865	0.0
6	0.0	0.0	0.0	0.0013785687699030865	0.0
7	0.0	0.0	0.0	0.0013785687699030865	0.0
8	0.0	0.0	0.0	0.0013785687699030865	0.0
9	0.0	0.0	0.0	0.0013785687699030865	0.0
10	0.0	0.0	0.0	0.0018380916932041155	0.0
11	0.0	0.0	0.0	0.0018380916932041155	0.0
12	0.0	0.0	0.0	0.002757137539806173	0.0
13	0.0	0.0	0.0	0.002757137539806173	0.0
14	0.0	0.0	0.0	0.002757137539806173	0.0
15	0.0	0.0	0.0	0.002757137539806173	0.0
16	0.0	0.0	0.0	0.002757137539806173	0.0
17	0.0	0.0	0.0	0.002757137539806173	0.0
18	0.0	0.0	0.0	0.002757137539806173	0.0
19	0.0	0.0	0.0	0.002757137539806173	0.0
20	0.0	0.0	0.0	0.00413570630970926	0.0
21	0.0	0.0	0.0	0.004595229233010288	0.0
22	0.0	0.0	0.0	0.011488073082525722	0.0
23	0.0	0.0	0.0	0.011488073082525722	0.0
24	0.0	0.0	0.0	0.01194759600582675	0.0
25	0.0	0.0	0.0	0.013785687699030866	0.0
26	0.0	0.0	0.0	0.015623779392234981	0.0
27	0.0	0.0	0.0	0.022516623241750414	0.0
28	0.0	0.0	0.0	0.034923742170878194	0.0
29	0.0	0.0	0.0	0.04824990694660803	0.0
30	0.0	0.0	0.0	0.059737980029133754	0.0
31	0.0	0.0	0.0	0.07352366772816461	0.0
32	0.0	0.0	0.0	0.0923641075835068	0.0
33	0.0	0.0	0.0	0.13326164775729837	0.0
34	0.0	0.0	0.0	0.18656630686021772	0.0
35	0.0	0.0	0.0	0.270199478901005	0.0
36	0.0	0.0	0.0	0.4126515851243239	0.0
37	0.0	0.0	0.0	0.620355946456389	4.5952292330102886E-4
38	0.0	0.0	0.0	0.8413864725641839	4.5952292330102886E-4
39	0.0	0.0	0.0	1.1350216205535413	4.5952292330102886E-4
40	0.0	0.0	0.0	1.4258996310030927	4.5952292330102886E-4
41	0.0	0.0	0.0	1.7608918420895427	4.5952292330102886E-4
42	0.0	0.0	0.0	2.134024455809978	4.5952292330102886E-4
43	0.0	0.0	0.0	2.5668950495595473	4.5952292330102886E-4
44	0.0	0.0	0.0	3.053070302412036	4.5952292330102886E-4
45	0.0	0.0	0.0	3.633447754541235	4.5952292330102886E-4
46	0.0	0.0	0.0	4.22531327975296	4.5952292330102886E-4
47	0.0	0.0	0.0	4.882890582996732	4.5952292330102886E-4
48	0.0	0.0	0.0	5.473377539438555	4.5952292330102886E-4
49	0.0	0.0	0.0	6.090057302508535	4.5952292330102886E-4
50	0.0	0.0	0.0	6.610237251685301	4.5952292330102886E-4
51	0.0	0.0	0.0	7.137769567634882	4.5952292330102886E-4
52	0.0	0.0	0.0	7.6804661400533965	4.5952292330102886E-4
53	0.0	0.0	0.0	8.185941355684529	4.5952292330102886E-4
54	0.0	0.0	0.0	8.772752128739942	4.5952292330102886E-4
55	0.0	0.0	0.0	9.416543744284684	4.5952292330102886E-4
56	0.0	0.0	0.0	10.07090438706535	4.5952292330102886E-4
57	0.0	0.0	0.0	10.668743710279987	4.5952292330102886E-4
58	0.0	0.0	0.0	11.280828244116957	4.5952292330102886E-4
59	0.0	0.0	0.0	11.87131520055878	4.5952292330102886E-4
60	0.0	0.0	0.0	12.46593786331031	9.190458466020577E-4
61	0.0	0.0	0.0	13.006796344035623	9.190458466020577E-4
62	0.0	0.0	0.0	13.543519118451224	9.190458466020577E-4
63	0.0	0.0	0.0	14.119301341347413	9.190458466020577E-4
64	0.0	0.0	0.0	14.666133620075637	9.190458466020577E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATGGTC	20	0.0069445036	52.5	60
CTTGCGT	35	0.0012535271	40.0	49
CGATAGA	35	0.0012535271	40.0	37
TACGTAC	45	9.177328E-5	38.888885	10
CGTCCCA	55	6.6742195E-6	38.181816	50
CGTGCGA	40	0.0024151404	35.0	10
CTACGTA	50	1.7059395E-4	35.0	9
CGTAGCG	40	0.0024151404	35.0	15
TTGCGTG	40	0.0024151404	35.0	50
ATGCGAT	40	0.0024151404	35.0	26
TCGTCCC	65	2.0962394E-5	32.307693	49
GATGTAG	55	2.985543E-4	31.81818	1
CGGAATC	45	0.0043008635	31.111109	20
TTGGCAT	45	0.0043008635	31.111109	32
TGTTCGA	45	0.0043008635	31.111109	37
CGAATTC	45	0.0043008635	31.111109	41
ATTGCAC	45	0.0043008635	31.111109	32
CCGCAAG	45	0.0043008635	31.111109	11
TGCGTGA	45	0.0043008635	31.111109	51
CCCTAGT	60	4.971183E-4	29.166666	1
>>END_MODULE