FastQCFastQC Report
Wed 25 May 2016
SRR1781202_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781202_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences196133
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCGT33291.6973176365017617No Hit
TCTTATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCG4120.21006153987345322No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCC2660.13562225632606445No Hit
CTTATACACATCTCCGAGCCCACGAGACTGTGCCTTACCTCGTATGCCGT2010.10248147940428178No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGCGA252.8322102E-670.0000110
CGTTGAT150.002222146470.035
CGAGAAT207.912256E-570.014
TATGCCG5150.059.80582443
CGTCTTC5000.059.548
CCGTCTT5000.059.547
CGTATGC5250.059.33333241
GCCGTCT5050.058.91089246
TCGTATG5350.058.87850640
CTCGTAT5300.058.77358639
ATCTCGT4750.056.7368437
TCTCGTA4750.056.7368438
ATGCCGT5350.056.26168444
TACCTCG509.749783E-1056.00000436
GTCTTCT5350.055.6074849
CTTGAAA5100.055.58823857
GTATGCC5550.055.49549542
TCTTCTG5450.054.58715450
TGCCGTC5450.054.58715445
CCGCTAT200.006943485252.528
Produced by FastQC (version 0.11.5)