##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1781202_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	196133
Sequences flagged as poor quality	0
Sequence length	76
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.2802231139074	34.0	34.0	34.0	33.0	34.0
2	33.31107972651211	34.0	34.0	34.0	33.0	34.0
3	33.36517057302952	34.0	34.0	34.0	33.0	34.0
4	33.4333386018671	34.0	34.0	34.0	34.0	34.0
5	33.42326890426394	34.0	34.0	34.0	34.0	34.0
6	36.85967685193211	38.0	37.0	38.0	34.0	38.0
7	36.764878934192616	38.0	37.0	38.0	34.0	38.0
8	36.535830278433515	38.0	37.0	38.0	34.0	38.0
9	36.495648361061114	38.0	37.0	38.0	34.0	38.0
10-11	36.42222369514565	38.0	37.0	38.0	34.0	38.0
12-13	36.37600760708295	38.0	37.0	38.0	34.0	38.0
14-15	36.37801899731305	38.0	37.0	38.0	34.0	38.0
16-17	36.30719460774067	38.0	37.0	38.0	34.0	38.0
18-19	36.30554521676618	38.0	37.0	38.0	34.0	38.0
20-21	36.24810205319859	38.0	37.0	38.0	34.0	38.0
22-23	36.31250223062922	38.0	37.0	38.0	34.0	38.0
24-25	36.30004639708769	38.0	37.0	38.0	34.0	38.0
26-27	36.07286382199834	38.0	37.0	38.0	33.0	38.0
28-29	36.05649992607057	38.0	37.0	38.0	32.0	38.0
30-31	36.12462206767856	38.0	37.0	38.0	33.5	38.0
32-33	36.0534535238843	38.0	37.0	38.0	32.0	38.0
34-35	36.05861328792197	38.0	37.0	38.0	32.5	38.0
36-37	36.00973064196234	38.0	37.0	38.0	32.0	38.0
38-39	35.89433700601123	38.0	37.0	38.0	32.0	38.0
40-41	35.96878138813968	38.0	37.0	38.0	32.0	38.0
42-43	35.9891731631087	38.0	37.0	38.0	32.0	38.0
44-45	35.970183497932524	38.0	37.0	38.0	32.0	38.0
46-47	35.99080470904947	38.0	37.0	38.0	32.0	38.0
48-49	35.97473398153294	38.0	37.0	38.0	32.0	38.0
50-51	35.86825011599272	38.0	36.5	38.0	31.5	38.0
52-53	35.90366231077891	38.0	37.0	38.0	32.0	38.0
54-55	35.92745229002769	38.0	37.0	38.0	32.0	38.0
56-57	35.867673976332384	38.0	37.0	38.0	32.0	38.0
58-59	35.866162247046645	38.0	37.0	38.0	31.5	38.0
60-61	35.844750755864645	38.0	36.5	38.0	31.5	38.0
62-63	35.81916097749996	38.0	36.0	38.0	31.0	38.0
64-65	35.79887117415224	38.0	36.0	38.0	31.0	38.0
66-67	35.75264743821795	38.0	36.0	38.0	31.0	38.0
68-69	35.6686891038224	38.0	36.0	38.0	31.0	38.0
70-71	35.512466030703656	38.0	36.0	38.0	31.0	38.0
72-73	35.398155843228835	38.0	36.0	38.0	31.0	38.0
74-75	35.39548673604136	38.0	36.0	38.0	30.5	38.0
76	34.54611921502246	37.0	34.0	38.0	25.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	4.0
21	8.0
22	20.0
23	70.0
24	138.0
25	271.0
26	526.0
27	973.0
28	1654.0
29	2616.0
30	3968.0
31	5946.0
32	8291.0
33	11409.0
34	15815.0
35	22271.0
36	33147.0
37	89006.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.48579790244375	19.242044938893507	9.547602902112343	27.724554256550405
2	21.965706943757553	19.455675485512383	33.713857433476264	24.864760137253803
3	19.28997160090347	22.54337617840955	30.28047294437958	27.886179276307406
4	13.58924811225036	17.90672655799891	37.1446926320405	31.35933269771023
5	13.346555653561614	35.10526020608465	36.938200098912475	14.609984041441267
6	30.265687059291402	39.339121922369	16.93646658135041	13.458724436989186
7	28.143657620084326	29.171021704659594	21.821926957727662	20.86339371752841
8	24.950926157250436	36.67256402543172	18.356931266028663	20.01957855128918
9	23.97964646438896	14.547271494343125	20.517709921328894	40.95537211993902
10-11	24.67126898584124	25.35141969989752	28.034293056242447	21.943018258018792
12-13	24.29015005124074	22.770263035797136	27.146885021898402	25.792701891063718
14-15	22.681802654321302	23.80502006291649	23.316575996900063	30.196601285862144
16-17	24.179000984026146	27.825506161635218	24.311564091713276	23.68392876262536
18-19	24.045418160126037	26.43614282145279	25.399601290960728	24.11883772746045
20-21	22.471996043501093	26.22327706199365	25.321593000667914	25.983133893837344
22-23	22.92449511301005	26.85014760392183	25.684102114381567	24.541255168686554
24-25	23.83739605267854	27.07040630592506	25.61960506391071	23.472592577485685
26-27	23.18962132838431	27.46682098372023	25.925774856857338	23.41778283103812
28-29	22.908434582655644	25.90538053259778	27.578224979988068	23.607959904758506
30-31	24.052046315510395	25.854394721948882	26.915919299658903	23.17763966288182
32-33	23.535050195530584	26.398413321572605	25.93342272845467	24.13311375444214
34-35	23.10192573406821	25.496729260246877	27.121901974680444	24.279443031004472
36-37	22.84827132608995	26.68597329363238	26.77111959741604	23.694635782861628
38-39	22.285387976526135	26.542193307602496	27.410991521059692	23.761427194811684
40-41	22.456190442199937	25.035307673874364	28.009055079971247	24.49944680395446
42-43	23.58552614807299	25.237466413097238	27.281742491064737	23.89526494776504
44-45	21.811729795597884	26.83816593841934	27.661586780399016	23.688517485583763
46-47	23.104729953653898	25.384560476819303	26.91005593143428	24.600653638092517
48-49	22.597166208644133	25.18597074434185	27.27944812958554	24.93741491742848
50-51	21.547878225489846	25.197442551737854	28.32261781546196	24.932061407310346
52-53	20.99238781847012	25.124277913456684	29.847603411970447	24.035730856102745
54-55	20.564361938072633	25.50335741563123	28.753702844498374	25.178577801797758
56-57	21.74213416406214	25.339183105341785	27.474468855317564	25.44421387527851
58-59	20.764736173922795	25.294060662917513	29.253618717910808	24.687584445248888
60-61	21.53895570862629	26.22174748767418	27.25624958574029	24.983047217959243
62-63	19.97598568318437	27.544829274013043	27.239679197279397	25.23950584552319
64-65	20.499100100442046	27.873942681751668	26.42594565932301	25.201011558483273
66-67	20.507512759199116	27.727103547082848	26.07108441720669	25.694299276511344
68-69	19.9721617473857	28.212743393513584	25.949483258809074	25.865611600291636
70-71	20.797622021791334	27.305450893016474	26.15011242371248	25.74681466147971
72-73	20.930440058531712	25.747834377692687	26.36450775749109	26.95721780628451
74-75	20.72165316392448	26.0430422213498	26.36680211897029	26.86850249575543
76	21.40486302661969	25.743755512840778	26.25412347743623	26.5972579831033
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	4.0
15	12.0
16	28.0
17	40.0
18	60.5
19	104.0
20	151.0
21	175.0
22	226.0
23	382.5
24	588.5
25	689.0
26	804.0
27	983.5
28	1230.5
29	1413.0
30	1681.5
31	2141.5
32	2880.0
33	3427.0
34	3773.5
35	4551.5
36	5617.0
37	6251.0
38	6844.0
39	8297.5
40	10044.5
41	11653.0
42	12375.0
43	12724.5
44	12978.5
45	12726.5
46	12570.0
47	12328.0
48	11098.0
49	9757.0
50	9404.0
51	8598.0
52	7062.5
53	5981.0
54	5629.0
55	5327.5
56	4531.0
57	3652.5
58	3269.0
59	2889.0
60	2318.5
61	2102.5
62	2077.0
63	1959.0
64	1805.5
65	1745.0
66	1710.0
67	1700.0
68	1627.0
69	1512.5
70	1473.0
71	1475.0
72	1400.0
73	1264.0
74	1141.0
75	1079.0
76	1018.5
77	895.5
78	735.5
79	638.0
80	567.5
81	418.5
82	295.0
83	250.0
84	202.5
85	114.5
86	58.5
87	43.0
88	29.5
89	12.0
90	6.0
91	3.5
92	3.0
93	3.5
94	2.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	196133.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.54476809104026
#Duplication Level	Percentage of deduplicated	Percentage of total
1	61.85707448179375	25.69837814136326
2	19.24082323920327	15.987110787067959
3	7.534086864744793	9.390056747207252
4	3.079169888197538	5.1169359567232435
5	1.6629235546064824	3.4542886714627317
6	1.072616373967576	2.673695910428128
7	0.7584404108832518	2.205646168671259
8	0.6344881754476394	2.1087731284383557
9	0.5007179411656419	1.8721989670274763
>10	3.552888332535621	26.653852232923576
>50	0.09940723831964951	2.582431309366603
>100	0.006136249278990709	0.5593143428183937
>500	0.0	0.0
>1k	0.0012272498557981421	1.6973176365017617
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCGT	3329	1.6973176365017617	No Hit
TCTTATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCG	412	0.21006153987345322	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCC	266	0.13562225632606445	No Hit
CTTATACACATCTCCGAGCCCACGAGACTGTGCCTTACCTCGTATGCCGT	201	0.10248147940428178	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0010197162129779282	0.0
2	0.0	0.0	0.0	0.0010197162129779282	0.0
3	0.0	0.0	0.0	0.0010197162129779282	0.0
4	0.0	0.0	0.0	0.0015295743194668923	0.0
5	0.0	0.0	0.0	0.0015295743194668923	0.0
6	0.0	0.0	0.0	0.0015295743194668923	0.0
7	0.0	0.0	0.0	0.0015295743194668923	0.0
8	0.0	0.0	0.0	0.0015295743194668923	0.0
9	0.0	0.0	0.0	0.0015295743194668923	0.0
10	0.0	0.0	0.0	0.0020394324259558564	0.0
11	0.0	0.0	0.0	0.0020394324259558564	0.0
12	0.0	0.0	0.0	0.0030591486389337846	0.0
13	0.0	0.0	0.0	0.0030591486389337846	0.0
14	0.0	0.0	0.0	0.0030591486389337846	0.0
15	0.0	0.0	0.0	0.0030591486389337846	0.0
16	0.0	0.0	0.0	0.0030591486389337846	0.0
17	0.0	0.0	0.0	0.0030591486389337846	0.0
18	0.0	0.0	0.0	0.0030591486389337846	0.0
19	0.0	0.0	0.0	0.004078864851911713	0.0
20	0.0	0.0	0.0	0.0056084391713786055	0.0
21	0.0	0.0	0.0	0.006118297277867569	0.0
22	0.0	0.0	0.0	0.007647871597334462	0.0
23	0.0	0.0	0.0	0.007647871597334462	0.0
24	0.0	0.0	0.0	0.007647871597334462	0.0
25	0.0	0.0	0.0	0.009687304023290318	0.0
26	0.0	0.0	0.0	0.011726736449246175	0.0
27	0.0	0.0	0.0	0.019374608046580637	0.0
28	0.0	0.0	0.0	0.03314077692178267	0.0
29	0.0	0.0	0.0	0.048946378222940554	0.0
30	0.0	0.0	0.0	0.0596533984592088	0.0
31	0.0	0.0	0.0	0.07596885786685566	0.0
32	0.0	0.0	0.0	0.09789275644588111	0.0
33	0.0	0.0	0.0	0.14377998602988787	0.0
34	0.0	0.0	0.0	0.19986437774367394	0.0
35	0.0	0.0	0.0	0.2895994044857316	0.0
36	0.0	0.0	0.0	0.44663570128433255	0.0
37	0.0	0.0	0.0	0.6781112816303223	0.0
38	0.0	0.0	0.0	0.9121361525087568	0.0
39	0.0	0.0	0.0	1.223149597467025	0.0
40	0.0	0.0	0.0	1.5397714815966717	0.0
41	0.0	0.0	0.0	1.888514426435123	0.0
42	0.0	0.0	0.0	2.285693891390026	0.0
43	0.0	0.0	0.0	2.7532337750404063	0.0
44	0.0	0.0	0.0	3.2702298950202158	0.0
45	0.0	0.0	0.0	3.879510332274528	0.0
46	0.0	0.0	0.0	4.519892114024667	0.0
47	0.0	0.0	0.0	5.217887861808059	0.0
48	0.0	0.0	0.0	5.849092197641396	0.0
49	0.0	0.0	0.0	6.4787669591552675	0.0
50	0.0	0.0	0.0	7.030433430376326	0.0
51	0.0	0.0	0.0	7.587708340768764	0.0
52	0.0	0.0	0.0	8.159769136249382	0.0
53	0.0	0.0	0.0	8.69155114131737	0.0
54	0.0	0.0	0.0	9.293693565080838	0.0
55	0.0	0.0	0.0	9.953449954877557	0.0
56	0.0	0.0	0.0	10.63614995946628	0.0
57	0.0	0.0	0.0	11.27653174121642	0.0
58	0.0	0.0	0.0	11.909775509475713	0.0
59	0.0	0.0	0.0	12.530782683179272	0.0
60	0.0	0.0	0.0	13.144141985285495	0.0
61	0.0	0.0	0.0	13.72283093615047	0.0
62	0.0	0.0	0.0	14.285204427607797	0.0
63	0.0	0.0	0.0	14.886327135158286	0.0
64	0.0	0.0	0.0	15.45481892389348	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTGCGA	25	2.8322102E-6	70.00001	10
CGTTGAT	15	0.0022221464	70.0	35
CGAGAAT	20	7.912256E-5	70.0	14
TATGCCG	515	0.0	59.805824	43
CGTCTTC	500	0.0	59.5	48
CCGTCTT	500	0.0	59.5	47
CGTATGC	525	0.0	59.333332	41
GCCGTCT	505	0.0	58.910892	46
TCGTATG	535	0.0	58.878506	40
CTCGTAT	530	0.0	58.773586	39
ATCTCGT	475	0.0	56.73684	37
TCTCGTA	475	0.0	56.73684	38
ATGCCGT	535	0.0	56.261684	44
TACCTCG	50	9.749783E-10	56.000004	36
GTCTTCT	535	0.0	55.60748	49
CTTGAAA	510	0.0	55.588238	57
GTATGCC	555	0.0	55.495495	42
TCTTCTG	545	0.0	54.587154	50
TGCCGTC	545	0.0	54.587154	45
CCGCTAT	20	0.0069434852	52.5	28
>>END_MODULE