FastQCFastQC Report
Wed 25 May 2016
SRR1781201_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781201_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences186080
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTCG207.9111895E-570.0000115
GTTTCGT252.3858136E-456.00000416
CCCGTTT252.3858136E-456.00000413
CTTGATA200.006942927452.50000424
AACTCTC305.8666355E-446.66666819
CCGTTTC305.8666355E-446.66666814
CGTGCAA350.001253058240.00000420
GATCTAA350.001253058240.00000415
TAGCGAG459.172193E-538.8888928
ATCCCGT459.172193E-538.8888911
ACGTTCT400.002414242435.00000439
ATCGTGA400.002414242435.00000413
GTGCTAG400.002414242435.00000427
CAAGAAT400.002414242435.00000424
GCTTTAA400.002414242435.0000041
GATCCCG652.0946136E-532.30769310
CTTGCTA552.9838886E-431.81818436
CGAGGTT552.9838886E-431.81818422
CATGTAC552.9838886E-431.8181843
GTGCAAG450.004299274631.11111321
Produced by FastQC (version 0.11.5)